Abstract: 【Objective】To isolate and identify chitinase high-yielding strains from natural environment soil samples， carry out enzymatic characterization of chitinase，and evaluate the efficacy of the strain in chitin degradation，which could provide theoretical basis for the industrial transformation of chitin-rich shrimp shells and other waste resources，as well as the realization of chitin resources in the green application of environmental protection.【Method】The chitinase high-yielding strains were screened by the colloidal chitin plate hydrolysis circle method，and identified by physiological and biochemical characteristics analysis and molecular biological identification techniques. The enzyme activity characteristics and fermentation conditions were studied using the 3,5-dinitrosalicylic acid（DNS）method，and the degradation rates of colloidal chitin and shrimp shells were determined by weight loss method.【Result】A chitinase high-yield strain was successfully isolated from the soil samples. After identification，the strain belonged to genus Chitinibacter，and was named as Chitinibacter sp. strain CQNU6-1. The enzyme activity analysis showed that the optimal culture conditions for this strain were 30 ℃ and pH 7.0，under which the chitinase activity reached 0.547 U/mL after 120 h. The chitinase produced by this strain showed the highest relative enzyme activity at 40 ℃ and pH 6.0，and it could still maintain more than 70.0% of the relative enzyme activity under the high temperature of 70 ℃ and the strong alkaline environment of pH 10.0，showing good thermal stability and acid-base adaptability. At ion concentrations of 0.01 and 0.1 mol/L，Na⁺，K⁺， Mg²⁺，Ca²⁺，Fe³⁺ and Mn²⁺ could enhance the relative enzyme activity of chitinase，among which the enhancement effect of Mn²⁺ was the most obvious，whereas the inhibitory effect of Cu²⁺ on relative enzyme activity was observed. In addition，CQNU6-1 strain was able to grow in the medium with chitin as the sole carbon source and showed significant degradation effects on colloidal chitin and chitin-rich shrimp shells，with degradation rates of up to 100.0% and 78.7% respectively. Meanwhile，the chitinase produced by this strain could be preliminarily enriched and purified by ammonium sulfate precipitation method.【Conclusion】The screened chitinase high-yielding strain Chitinibacter sp. strain CQNU6-1 exhibits wide adaptability to fermentation temperature and pH. It demonstrates good chitin biodegradation ability under hightemperature and alkaline conditions.