4个洋紫荆品种花色与花香的相关性

Correlation analysis between flower colors and floral fragrance in 4 Bauhinia variegate varieties

  • 摘要: 【目的】 研究洋紫荆花色分布规律及花香化合物的释放与花色的相关性,为洋紫荆的色香调控及育种改良提供理论依据。【方法】 采用顶空固相微萃取(HS-SPME)与气相色谱—质谱联用(GC-MS)技术检测YZJ1号(紫红色)、YZJ2号(粉红色)、YZJ3号(白色带黄斑)和YZJ4号(白色带红斑)4个洋紫荆品种的花香挥发性物质,利用色差仪测量花朵花色参数亮度值(L*)、红度值(a*)、黄度值(b*)和彩度值(C*),通过偏最小二乘回归(PLS)分析花香成分与花色之间的相关性。【结果】 4个洋紫荆品种之间的花色参数值区别较明显;从花瓣主色上看,YZJ3号和YZJ4号之间颜色接近,但可以通过主花瓣心部主色区分;不同花色主花瓣心部主色之间区别明显。4个洋紫荆品种共鉴定出33种花香成分,主要由单萜类化合物和倍半萜类化合物组成,二者相对含量占比达95.00%以上;苯丙烷/苯环型化合物占比非常小,其在YZJ4号的相对含量占比高于其他3个品种。倍半萜类化合物α-石竹烯及单萜类化合物异松油烯、γ-松油烯与主花瓣心部主色a*2、C*2以及花瓣主色a*1和C*1呈显著正相关(|PLS相关系数|≥0.80,下同),单萜类化合物2,6二亚甲基辛-7-烯-3-酮、β-月桂烯与花色参数C*2、a*1和C*1呈显著正相关,单萜类化合物(Z)-β-罗勒烯与花色参数a*1和C*1呈显著正相关;花瓣主色L*1则与α-石竹烯、异松油烯、γ-松油烯、2,6-二亚甲基辛-7-烯-3-酮、β-月桂烯、(Z)-β-罗勒烯呈显著负相关,主花瓣心部主色L*2也与α-石竹烯、异松油烯、γ-松油烯呈显著负相关。【结论】 4个洋紫荆品种花朵挥发性化合物中部分萜类化合物与花瓣主色、主花瓣心部主色的红度和彩度呈正相关,花瓣颜色越红越鲜艳,萜类化合物释放量越多;而部分萜类化合物与花瓣主色和主花瓣心部主色的亮度呈负相关,花瓣颜色亮度越高,萜类化合物释放量越少。

     

    Abstract: 【Objective】 This study aimed to investigate the distribution patterns of flower color and the correlation between floral fragrance volatile compounds and color in Bauhinia variegate,which could provide theoretical basis for color and fragrance regulation and breeding improvement in B.variegata.【Method】Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was employed to analyze the floral fragrance volatile compounds of 4 B.variegata varieties:YZJ1 (purple-red),YZJ2 (pink),YZJ3 (white with yellow spots),and YZJ4 (white with red spots).Color parameters of the flowers,including lightness (L*),redness (a*),yellowness (b*),and chroma (C*),were measured using a colorimeter.Partial least squares regression (PLS) was applied to assess the correlation between floral compounds and flower color. 【Result】 Great differences in color parameters were observed among the 4 B.variegata varieties.While the petal colors of YZJ3 and YZJ4 appeared similar,they could be distinguished by the dominant color of the main petal heart,which exhibited distinct variations across varieties.A total of 33 floral compounds were identified,predominantly composed of monoterpenes and sesquiterpenes,accounting for over 95.00% of the total relative content.Phenylpropanoids/benzenoids were present in minimal amounts,with YZJ4 exhibiting a higher relative content of these compounds compared to the other 3 varieties.There was significant positive correlations(|PLS correlation coefficient|≥0.80,the same below) between sesquiterpenoid α-caryophyllene,monoterpenoids terpinolene and γ-terpinene and a*2 and C*2 of main color of main petals heart,as well as a*1 and C*1 of main color of petals.Monoterpenoids such as 2,6-dimethylocta-7-en-3-one and β-myrcene showed positive correlations with flower color parameters C*2,a*1 and C*1,while monoterpenoid (Z)-β-ocimene was positively correlated with flower color parameters a*1 and C*1.Conversely,L*1 of main color of petals exhibited significant negative correlations with α-caryophyllene,terpinolene,γ-terpinene,2,6-dimethylocta-7-en-3-one,β-myrcene and (Z)-β-ocimene.Similarly,L*2 of main color of the main petals heart was negatively correlated with α-caryophyllene,terpinolene and γ-terpinene. 【Conclusion】 Some of the terpenoids in the flower volatile compounds of 4 B.variegata varieties are positively correlated with the redness and chroma of the main color of petals and the main color of main petal heart.The redder the petal color is,the more terpenoids are released.Some terpenoids are negatively correlated with the brightness of the main color of petals and the main color of main petal heart.The higher the brightness of the petal color,the less the release of terpenoids.

     

/

返回文章
返回