Abstract: 【Objective】 To clarify the pathogen causing Eustoma grandiflorum（ Raf.） Shinners symptoms of raised leaf veins and crumpled leaves in Nanning, Guangxi, and to analyzed the genetic evolutionary relationship of virus isolates, which could provide theoretical basis for the prevention and control of viral diseases in flower crops. 【Method】 A Eustoma grandiflorum（Raf.） Shinners with typical geminivirus symptoms, such as raised leaf veins and crumpled leaves, was used as the study object. The total DNA of Eustoma grandiflorum（Raf.） Shinners leaves was detected by PCR using geminivirus universal degenerative primers（PA/PB）. The whole genome sequence of related virus pathogens was ob-tained by designing back-to-back primers（ TbCSV-F/TbCSV-R） and gene cloning. The spliced sequences were compared and analyzed by BLASTn, and the whole genome sequence and genetic evolution of related virus pathogens were ana-lyzed by biological softwares SnapGene, SDT and MEGA 7.0. 【Result】 PA/PB primer was used to amplify one 500 bp specific target band from the samples. BLASTn analysis showed that the nucleotide sequence similarity between the ob-tained sequences and the isolates of tobacco curly shoot virus（ TbCSV） registered in GenBank was more than 91%. It was confirmed that the collected Eustoma grandiflorum（ Raf.） Shinners plants were infected by Begomovirus virus. β satellite universal primers（ β01/β02） could not be used to amplify the bands, confirming that β-satellite molecules were not pre-sent in the leaves of infected Eustoma grandiflorum（ Raf.） Shinners. Tbcsv-f/TBCSV-R was used to amplify the total DNA of infected samples by PCR. The obtained product was purified, cloned, sequenced and sequentially splicing to obtain a complete viral genome sequence with a length of 2743 bp. The nucleotide similarity analysis on the obtained sequence in-dicated that the viral isolate was a TbCSV isolate, named TbCSV-GX-YJG. Amino acid homology analysis showed that the nucleotide and amino acid sequence similarity of the positive chain encoding AV1 and AV2 genes with TbCSV-SHJ01（GenBank accession number MW779539.1） was the highest, reaching 100%. The results of evolutionary tree analysis showed that TbCSV-GX-YJG and isolates from Guangxi（ TbCSV-SHJ01） and Sichuan（ TbCSV-SC740, GenBank acces-sion number MH165181.1） clustered in the same small branch, indicating a close genetic relationship; and isolates from Yunnan（ TbCSV-CN, GenBank accession number KM383752.1）, Bangladesh（ TbCSV-YT8, GenBank accession num-ber HG003650.1）, India（TbCSV-WSF1 and TbCSV-TC240, Genbank accession numbers were KM383752.1 and KF551584.0） were in different branches, indicating that TbCSV isolates had certain genetic diversity. 【Conclusion】 TbCSV of Begomovirus is the viral pathogen that infects Eustoma grandiflorum（Raf.） Shinners displaying raised leaf veins and crumpled leaves in Guangxi.