红带滑胸针蓟马(缨翅目:蓟马科)线粒体基因组全序列测定与分析

Whole sequencing and analysis of mitochondrial genome of Selenothrips rubrocinctus( Thysanoptera: Thripidae)

  • 摘要: 【目的】 明确红带滑胸针蓟马(Selenothrips rubrocinctus)线粒体基因组结构特征,并基于线粒体基因组序列分析其系统发育关系,为滑胸针蓟马属昆虫的分子鉴定和系统发育研究提供分子生物学依据。【方法】 利用第二代测序技术Illumina平台对红带滑胸针蓟马的线粒体基因组进行测序、组装和注释;基于18种蓟马科和1种管蓟马科外群昆虫的线粒体基因组13个蛋白质编码基因(PCGs)序列,采用最大似然法(ML)和贝叶斯法(BI)构建系统发育进化树,分析红带滑胸针蓟马与其他蓟马的系统发育关系。【结果】 红带滑胸针蓟马线粒体基因组全长14984 bp,包含37个基因(13个PCGs、22个tRNA基因、2个rRNA基因)和1个控制区。基因间隔区有19处,最长间隔区长度为25 bp;基因重叠区有10处,最长重叠区长度为23 bp。基因组拥有缨翅目中大部分昆虫线粒体基因组的基因组成和排列顺序,AT含量为72.9%,具有明显的AT偏好性。13个PCGs中,除cox1以TTG为起始密码子外,其余均以ATN为起始密码子;nad6以TAG为终止密码子,nad2cox2以不完整的T为终止密码子,其余10个基因以TAA为终止密码子。22个tRNA基因中,除trnRtrnS2trnY缺少TΨC环,trnS1trnV缺少DHU臂环外,其余17个基因具有完整的三叶草结构。系统发育分析结果显示,红带滑胸针蓟马与腹突皱针蓟马(Rhipiphorothrips cruentatus)聚在同一小分支上,二者的亲缘关系最近。【结论】 红带滑胸针蓟马的线粒体基因组结构、组成和基因排列顺序较保守,与蓟马科其他物种线粒体基因组结构和排列一致。针蓟马亚科为单系性,其中,红带滑胸针蓟马与腹突皱针蓟马的亲缘关系最近。

     

    Abstract: 【Objective】 To clarify the mitochondrial genome structure of Selenothrips rubrocinctus and analyze its phylogenetic relationship based on mitochondrial genome sequence, which could provide molecular evidence for identification and phylogeny of Selenothrips insects. 【Method】 The mitochondrial genome of Selenothrips rubrocinctus was sequenced by using Illumina next-generation sequencing, then assembled and annotated. Maximum likelihood(ML) and Bayesian inference(BI) methods were used to construct the phylogenetic tree of 18 Thripidae species and 1 Phlaeothripidae species based on the sequences of 13 protein-coding genes(PCGs) of mitochondrial genomes, to analyze the phylogenetic relationship between S. rubrocinctus and other thrips. 【Result】 The mitochondrial genome of Selenothrips rubrocinctus was 14984 bp in length, including 37 genes(13 PCGs, 22 tRNA genes, 2 rRNA genes) and 1 non-coding region.There were 19 intergenic regions, and the longest intergenic region was 25 bp. There were 10 gene overlapping regions,and the longest overlapping region was 23 bp.The genome had the typical gene composition and order of mitochondrial genome from most Thysanoptera insects, and the AT content was 72.9%, showing obvious AT bias. Among the 13 PCGs,all genes used ATN as the start codon, except for cox1 used TTG as the start codon; and except for nad6 had TAG as the stop codon, 2 genes(nad2 and cox2) had incomplete T as the stop codon, another 10 used TAA as the stop codon.Among the 22 tRNA genes, the 17 tRNA genes could form the complete clover-leaf secondary structures, except trnR,trnS2, and trnY lacked TΨC ring, trnS1 and trnV lacked DHU ring. Phylogenetic analysis showed that Selenothrips rubrocinctus clustered on the same small branch with Rhipiphorothrips cruentatus, and they were closely related.【Conclusion】 The mitochondrial genome structure, composition and gene sequence of Selenothrips rubrocinctus are conserved, consistent with that of other species of Thripidea. Panchaetothripinae is monophyletic and Selenothrips rubrocinctus is closely related to Rhipiphorothrips cruentatus.

     

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