Abstract: 【Objective】To clarify the composition of non-native fish species in inland waters of Guangxi at the present stage，and to explore the distributional characteristics from the similarities and differences of each water system，which could provide scientific basis for the prevention and control of non-native fish invasion in Guangxi.【Method】Taking inland waters of Guangxi as the study area，a total of 34 sampling sites were set up in the main water systems. Non-native fish samples were collected by fishing boats in 4 consecutive quarters：February 2023（spring），May-June 2023（summer），August-September 2023（autumn），and November-December 2023（winter）. The Jaccard similarity coefficient （Jsc）were used to analyze the similarity of non-native fishes in different water systems，and the differences in the structure of non-native fishes in water systems were discussed using principal coordinates analysis（PCoA）and permutation multivariate analysis of variance（PERMANOVA）.【Result】A total of 7690 non-native fishes were collected，belonging to 7 orders，13 families，20 genera and 23 species，of which 9 species of Cypriniformes were the most numerous，accounting for 39.13% of the total；19 species were introduced for aquaculture purposes，accounting for 82.61% of the total；7 species from Asia and 7 species from North America，each accounting for 30.43% of the total. From the distribution of non-native fish in the inland waters of Guangxi，Hongshuihe River had the highest number of non-native fish species， with 16 species，and the lowest was in Nanpanjiang River，with 7 species. The non-native fish species with the widest range of distributional samples were Oreochromis niloticus and Coptodon zillii，both with 97.06%. And C. zillii had the highest frequency of occurrence，with 89.71%. In terms of non-native fishes distribution similarity level，Jsc showed that the very similar water systems were Xunjiang River-Qianjiang River（Jsc=0.93），Xunjiang River-Youjiang River（Jsc= 0.93），Hongshuihe River-Qianjiang River（Jsc=0.81），Hongshuihe River-Xunjiang River（Jsc=0.76），and the very dissimilar water systems were Nanpanjiang River-Hejiang River（Jsc=0.23）. PCoA showed that Xunjiang River，Qianjiang River，Liujiang River，Hejiang River，Nanpanjiang River，Yongjiang River-Yujiang River，and Youjiang River were clearly separated from each other.【Conclusion】A total of 23 non-native fish species are collected in inland waters of Guangxi，most of which are introduced by aquaculture. In terms of distributional characteristics，there are more nonnative fish species in the main streams and fewer non-native species in the tributaries. There are similarities in the species composition of non-native fishes in each water systems，and the C. zillii，O. niloticus，Sarotherodon galilaea，and Clarias lazera have been widely distributed. Therefore，there is an urgent need to further strengthen the management of farmed non-native fish species to prevent the escape of newly introduced non-native fish and the spread of escaped non-native fish.