叉角厉蝽3龄若虫肠道可培养细菌多样性及促生潜力分析

Diversity and plant growth promoting potential of gut culturable bacteria in third-instar nymphs of Eocanthecona furcellata(Wolff)

  • 摘要: 【目的】 了解叉角厉蝽若虫肠道可培养细菌的组成,分析可培养细菌的促生长特性,为叉角厉蝽肠道细菌与宿主、植物间的关系研究及有益菌株的开发利用提供科学依据和研究材料。【方法】 采用传统的细菌分离培养法分离、纯化叉角厉蝽若虫肠道细菌,通过细菌形态特征、染色反应、生理生化试验和16S rDNA序列分析对分离到的肠道细菌进行分类鉴定,测定其固氮、溶磷、产吲哚乙酸和产铁载体能力,定性分析分离菌株的促生潜力。【结果】 从叉角厉蝽若虫肠道中分离到11株细菌,其中革兰氏阳性菌8株、革兰氏阴性菌3株;细胞形态3株为短杆状、1株为球状、7株为杆状。经鉴定11株肠道细菌分属于2门2纲3目4科4属,优势门、纲、目、科、属分别为厚壁菌门(Firmicutes,85.67%)、芽孢杆菌纲(Bacilli,85.67%)、乳杆菌目(Lactobacillales,75.80%)、肠球菌科(Enterococcaceae,75.80%)、肠球菌属(Enterococcus,75.80%);在种分类水平由粪肠球菌(E. faecalis,75.80%)、枯草芽孢杆菌(Bacillus subtilis,9.17%)、摩氏摩根氏菌(Morganella morganii,6.51%)、嗜线虫沙雷氏菌(Serratia nematodiphila,4.49%)、黏质沙雷氏菌(S. marcescens,3.30%)、苏云金芽孢杆菌(B. thuringiensis,0.28%)、贝莱斯芽孢杆菌(B. velezensis,0.27%)和高地芽孢杆菌(B. altitudinis,0.18%)8种组成。11株分离菌株的植物促生特性分析结果表明,2株沙雷氏菌促生潜力最大,具有固氮、溶磷、产吲哚乙酸和产铁载体4种促生活性,其次为摩根氏菌,具有溶磷、产吲哚乙酸和产铁载体3种促生活性。【结论】 叉角厉蝽若虫肠道可培养细菌种类较丰富,分离获得的11株细菌均具有不同程度的促生长特性。

     

    Abstract: 【Objective】 To study the composition of culturable bacteria in the gut of nymphs of the Eocanthecona furcellata(Wolff),and analyze the growth promoting characteristics of the strains,so as to provide scientific basis and research materials for the study of the relationship among the gut bacteria of E. furcellata,hosts and plants,as well as the development and utilization of probiotic. 【Method】 The traditional bacterial isolation and culture method was used to isolate and purify the gut bacteria of nymphs of E. furcellata. The isolated gut bacteria were classified and identified by bacterial morphology,staining reaction,physiological and biochemical tests and 16S rDNA sequence analysis,and qualitative analysis of the growth promoting potential of the strains was conducted by determining their nitrogen fixation,phosphorus solubilization,indoleacetic acid production and siderophore production ability. 【Result】 A total of 11 strains of bacteria were isolated from the gut of nymphs of E. furcellata,the 11 strains of gut bacteria included 8 Gram-positive bacteria and 3 Gram-negative bacteria strains. The cell morphology of 3 strains was short rod-shaped,1 stain was globular,and 7 strains were rod-shaped. and the 11 strains of gut bacteria bebonged to 2 phyla,2 classes,3 orders,4 families and 4 genera. The dominant phylum,class,order,family and genus were Firmicutes(85.67%),Bacilli(85.67%),Lactobacillales (75.80%),Enterococcaceae(75.80%),Enterococcus(75.80%)respectively. At the species level,E. faecalis(75.80%), Bacillus subtilis(9.17%),Morganella morganii(6.51%),Serratia nematodiphila(4.49%),S. marcescens(3.30%),B. thuringiensis(0.28%),B. velezensis(0.27%)and B. altitudinis(0.18%) were identified as abundant bacterial species. Plant promoting characterization of the 11 isolated strains showed that 2 strains of Serratia had the highest promotion potential with 4 plant growth promoting properties:nitrogen fixation,phosphorus solubilization,indoleacetic acid production and siderophore production,followed by 1 strain of Morganella with 3 plant growth promoting properties:phosphorus solubilization,indoleacetic acid production and siderophore production. 【Conclusion】 The culturable bacterial species in the gut of nymphs of E. furcellata are relatively abundant,and 11 bacterial strains isolated from the gut have different degrees of growth promoting properties.

     

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