河南省纤毛鹅观草种质资源表型多样性分析及综合评价

Phenotypic diversity and comprehensive evaluation of Roegneria ciliaris(Trin.)Nevski germplasm resources in Henan

  • 摘要: 【目的】 分析纤毛鹅观草种质资源的表型多样性,筛选作为饲草利用的优良种质,为纤毛鹅观草种质挖掘和新品种培育提供优异资源和理论参考。【方法】 以来自河南地区不同生态区域的15份野生纤毛鹅观草种质为材料,对其16个表型性状进行变异分析、相关分析、聚类分析和主成分分析,并以各主成分特征值贡献率为权重建立综合得分(D)评价数学模型,对纤毛鹅观草种质进行综合评价排序。【结果】 16个表型性状在不同纤毛鹅观草种质间存在极显著差异(P<0.01,下同),旗叶宽度的变异系数最大(21.88%),其次为种子产量(17.88%)和旗叶长度(17.81%),种子宽度的变异最小,仅为5.67%;7个营养相关性状的平均变异系数为15.48%,其余9个繁殖相关性状的平均变异系数为10.82%。种子产量与小穗宽度、芒长度和千粒重显著相关(P<0.05,下同),而干草产量与旗叶宽度、叶鞘长度、花序长度、小穗数、小穗宽度和种子长度显著正相关,与小穗长度和芒长度极显著正相关。15份纤毛鹅观草种质资源在欧式距离10处可分为4个类群,第I类群包括5份种质,主要性状特征是植株较高,穗秆和花序较长,旗叶长宽中等;第II类群包括2份种质,主要特征是植株较矮,繁殖性状较为突出;第III类群包括3份种质,主要特征是旗叶较大,第一节间较长,干草产量较高,但其种子较小,种子产量低;第IV类群包括5份种质,主要特征是植株矮小。旗叶宽度、叶鞘长度、芒长度、小穗数和小穗长度是引起纤毛鹅观草不同居群表型性状差异的主要因子。【结论】 河南南阳的4个种质资源(编号为RC13、RC10、RC15、RC12)综合性状表现较好,这些种质株高更高,旗叶更宽,饲草产量和种子产量相对更高,可用作纤毛鹅观草育种亲本材料或直接生产利用。

     

    Abstract: 【Objective】 To analyze the phenotypic diversity of Roegneria ciliaris(Trin.)Nevski germplasm resources, and to screen the excellent germplasms for forage utilization,which could provide excellent resources and theoretical reference for R. ciliaris germplasms exploration and new variety breeding. 【Method】 The 16 phenotypic traits of 15 wild R. ciliaris populations collected from different ecological regions in Henan were subjected to variation analysis,correlation analysis,cluster analysis and principal component analysis,and the comprehensive score(D)evaluation mathematical model was established with the contribution rate of each principal component characteristic value as the weight to comprehensively evaluate and rank the R. ciliaris germplasm resources. 【Result】 There were extremely significant differences in 16 phenotypic traits among different R. ciliaris germplasms(P<0.01,the same below). The variation coefficient of flag leaf width was the largest(21.88%),followed by seed yield(17.88%)and flag leaf length(17.81%). The variation of seed width was the smallest(only 5.67%). The average variation coefficients of 7 nutrition-related traits was 15.48%,and the average variation coefficient of the other 9 reproduction-related traits was 10.82%. Correlation analysis showed that seed yield was significantly affected by spikelet width,awn length and 1000-seed weight(P<0.05,the same below),while hay yield was significantly and positively correlated with flag leaf width,leaf sheath length,inflorescence length,spikelet number,spikelet width and seed length,extremely significantly correlated with spikelet length and awn length. Through cluster analysis,the 15 R. ciliaris germplasm resources could be classified into 4 taxa at Euclidean distance 10. The I taxon included 5 germplasms. The main trait characteristics were taller plants,longer spike rod and inflorescence,and medium length and width of flag leaves. The II taxon included 2 germplasms,and the trait characteristics were more prominent reproduction-related traits and shorter plants. The III taxon included 3 germplasms,and the main trait characteristics were larger flag leaves,longer internode,higher hay yield and lower seed yield. The IV taxon included 5 germplasms,and the main characteristics were short plants. Principal component analysis showed that flag leaf width,leaf sheath length,awn length,spikelet number and spikelet length were the main factors causing differences in phenotypic traits among different populations of R. ciliaris. 【Conclusion】 The 4 germplasm resources originated from Nanyang,Henan(numbers as RC13, RC10,RC15,RC12)perform better in terms of overall traits,with higher plant,wider flag leaf,and higher forage yield and seed yield,which are expected to be used as parental materials for R. ciliaris breeding or be applied directly in production.

     

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