Abstract: 【Objective】 To investigate the effects of reduced nitrogen application on the nodulation，nitrogen fixation， absorption and utilization in rubber tree-kudzu intercropping after rhizobia inoculation，which could provide reference for minimizing nitrogen fertilizer application in rubber plantations and achieving green development. 【Method】 In the young rubber plantation，a randomized block design was implemented with 5 treatments set up：rubber tree monoculture，combined with standard nitrogen fertilizer application（control group，CK1），rubber tree intercropping kudzu without rhizo-bia inoculation（control group，CK2），and rubber tree intercropping kudzu with rhizobia inoculation and represented standard nitrogen fertilization（T100），25% nitrogen fertilization reduction（T75），and 50% nitrogen fertilization reduction （T50）respectively. Examined the impact of various treatments on the nitrogen fixation capabilities of nodules，the growth status of crops，the nitrogen content in the crops，the accumulation of nitrogen in the kudzu，and the overall nitrogen content present in the surface soil within the depth range of 0-30 cm. 【Result】 Compared with T100 treatment，both T75 and T50 treatments demonstrated a notable increase in the quantity of root nodules，root nodule fresh weight，nitrogenase activity，nitrogen fixation potential，and leghemoglobin content of kudzu. A statistically significant difference existed between the T50 treatment and the T100 treatment（P<0.05，the same below）. Furthermore，the shoot dry weight and root dry weight of kudzu in the T75 and T50 treatments did not exhibit significant difference when compared to the CK2 （P>0.05，the same below）. Compared to CK2，there was significant increase in the nitrogen content of the kudzu roots and the nitrogen accumulation in shoot，which were as 1.10 and 1.49 times as those of CK2. In comparison to CK1 and CK2，the inoculation of rhizobia with reduced nitrogen fertilizer treatment did not yield significant impact on rubber tree circumference，tree height，the nitrogen content in tree leaves. However，compared to CK1，there was significant increase of over 15% in the total nitrogen content in the surface soil（0-10 cm，10-20 cm and 20-30 cm）of the rubber plantation under the T50 treatment. The correlation analysis indicated significant correlation between the activity of nitrogenase activity in nodules and the dry weight，nitrogen content，and nitrogen accumulation of kudzu. 【Conclusion】 Inocu‐ lating rhizobia with decreasing 50% nitrogen fertilizer application in the rubber tree-kudzu intercropping system can improve the nitrogen-fixing symbiosis of kudzu root nodules，increase the total nitrogen content in the surface soil，promote kudzu to absorb and accumulate more nitrogen nutrients，and maintain the nitrogen content in rubber tree leaves，which can ensure the normal growth of kudzu and rubber tree.