光合细菌沼泽红假单胞菌Atp2蛋白对水稻生长及抗逆的影响

Effects of photosynthetic bacteria Rhodopseudomonas palustris Atp2 protein on growth and stress resistance in rice

  • 摘要: 【目的】 探究光合细菌沼泽红假单胞菌PSB06 Atp2蛋白对水稻生长、防御酶活性及防御相关基因转录水平的影响,为生防菌在水稻上的推广应用提供理论依据。【方法】 以水稻品种CO-39为试验材料,用0(蒸馏水,CK)、20和50 μg/mL Atp2蛋白3种处理液对水稻种子和叶片进行处理,对生长14 d的水稻体内叶绿素a/b和过氧化氢(H2O2)含量,以及过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱氨肽还原酶(GR)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)等水稻防御酶活性进行检测;运用实时荧光定量PCR检测水稻体内防御相关基因(OsWRKY70、OsLOX1、OsAOS2、OsPR1a、OsCHT1OsPAL1)的转录水平。【结果】 与CK相比,Atp2蛋白处理水稻叶片后,其体内叶绿素a/b显著(P<0.05,下同)或极显著(P<0.01,下同)升高,而处理种子后叶绿素a/b无显著变化(P>0.05);Atp2蛋白处理后水稻体内H2O2含量均升高,其中处理叶片后H2O2含量极显著升高;Atp2蛋白处理后水稻体内POD和CAT活性显著或极显著下降;Atp2蛋白处理后水稻体内SOD、GR和PAL活性均极显著升高;Atp2蛋白处理种子后水稻体内PPO活性显著或极显著升高,而处理叶片后PPO活性显著或极显著下降。Atp2蛋白处理后水稻防御相关基因OsWRKY70、OsLOX1、OsAOS2(50 μg/mL Atp2蛋白处理水稻叶片外)、OsPR1aOsCHT1表达均上调,其中OsWRKY70、OsLOX1OsCHT1基因表达显著或极显著上调;Atp2蛋白处理种子后OsPAL1基因表达极显著上调,而处理叶片后OsPAL1基因表达极显著下调。【结论】 沼泽红假单胞菌PSB06 Atp2蛋白对水稻有较好的促生抗逆效果,具有推广应用潜力。

     

    Abstract: 【Objective】 To investigate the effects of the photosynthetic bacteria Rhodopseudomonas palustris PSB06 Atp2 protein on the growth,defence enzyme activity and the transcript levels of defense-related genes in rice,and to provide theoretical basis for the popularization of the use of biocontrol bacteria in rice. 【Method】 Rice variety CO-39 was used as the test material,and rice seeds and leaves were treated with 3 treatment solutions of 0(distilled water,CK),20 and 50 μg/mL Atp2 protein,the chlorophyll a/b and the hydrogen peroxide(H2O2)content were detected,as well as the activities of rice defense-related enzymes such as peroxidase(POD),catalase(CAT),superoxide dismutase(SOD), glutathione reductase(GR),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO)in rice,and the transcript levels of defense-related genes(OsWRKY70,OsLOX1,OsAOS2,OsPR1a,OsCHT1 and OsPAL1)in rice were detected by real-time fluorescence quantitative PCR. 【Result】 Compared with CK,chlorophyll a/b was significantly(P<0.05,the same below)or extremely significantly(P<0.01,the same below)increased in rice after Atp2 protein treatment of rice leaves,while chlorophyll a/b did not change significantly after treatment of seeds(P>0.05);H2O2 content in rice was elevated in all Atp2 protein treatments,with extremely significant elevation in the content of H2O2 after treatment of leaves; POD and CAT activities in rice were significantly or extremely significantly decreased after Atp2 protein treatment;SOD, GR and PAL activities in rice were extremely significantly increased after Atp2 protein treatment;PPO activity in rice was significantly or extremely significantly increased after Atp2 protein treatment of seeds,whereas PPO activity was signifi-cantly or extremely significantly decreased after treatment of leaves. After Atp2 protein treatment,expressions of rice defense genes OsWRKY70,OsLOX1,OsAOS2(except 50 μg/mL Atp2 protein treated rice leaves),OsPR1a and OsCHT1 were all up-regulated,of which the expression of OsWRKY70,OsLOX1 and OsCHT1 genes were either significantly or extremely significantly up-regulated;the expression of OsPAL1 gene was extremely significantly up-regulated after the treatment of seeds with Atp2 protein,and OsPAL1 gene expression was extremely significantly up-regulated after the treatment of leaves. 【Conclusion】 The R. palustris PSB06 Atp2 protein of R. palustris has good growth-promoting and stressresistant effect on rice,which has the potential for promotion and application.

     

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