Abstract: 【Objective】Based on widely targeted metabolome，the differential metabolites of flavonoids in Vitis heyneana Roem. et Schult. var. adenoclada（Hand. -Mazz.）Yeniang No. 2 grape at different vine ages and under different cultivation conditions were studied，providing theoretical reference for improving grape and wine quality.【Method】 Yeniang No. 2 grapes at different vine ages and under different cultivation conditions（regions and trellis systems）were used as test materials to measure berry weight，pH，soluble solids，titratable acid content，brightness value（L^*），redgreen color value（a^*），yellow-blue color value（b^*）. Ultra high performance liquid chromatography-tandem mass spectrometer was used for widely targeted metabolome of grape berries at maturity stage.【Result】There were varying degrees of differences in the physicochemical indicators of Yeniang No. 2 grapes with diffe-rent vine ages and cultivation conditions. The regions had greater impact on the physicochemical indicators of grape fruits，and the fruits planted in the Xiangsihu demonstration vineyard had better maturity，but with smaller berries and lighter skin color. The trellis systems and vine ages had slight impact on the berry weight of grapes，but had great impact on the sugar and acid content. Grapes grown on shed frame in the same region had better fruit maturity than those grown on hedgerow frame，and the older the vines，the better the berry maturity of the plants. A total of 789 metabolites belonging to 11 ca-tegories were identified from mature grape berries of different vine ages and cultivation conditions，among which flavonoids were the most abundant，accounting for 187 species. There were a total of 271 differential metabolites among 12 samples，which were clustered into 7 groups. Flavonoids compounds had the highest proportion among all subtypes，especially group Ⅱ，which accounted for 59%，followed by group Ⅰ，which accounted for 49%. Differential metabolites could be used to clearly distinguish grape samples from different regions，trellis systems and vine ages，with the greatest diffe-rences between regional samples，and flavonoids were the main compounds that causing the differences. The cultivation of Mingyang demonstration vineyard was more conducive to the accumulation of flavonoids such as myricetin，quercetin，epicatechin and petunidin than the cultivation of Xiangsihu demonstration vineyard. Compared with the 8-year-old vine，the 5-year-old vine promoted the accumulation of kaempferol ，catechins and cyaniding，while the shed frame was more conducive to the accumulation of flavan-3-ol,cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside than hedgerow frame. The correlation analysis results showed that the content of soluble solids was significantly negatively correlated with the content of quercetin and myricetin in flavonols，and L^* and a^* were significantly negatively correlated with myricetin.【Conclusion】 The contents of flavonoid metabolites of V. heyneana Roem. et Schult. var. adenoclada（Hand. -Mazz.）Yeniang No. 2 grapes are different at different vine ages and under different cultivation conditions，and the influence of region is the most obvious.