Abstract: 【Objective】 This study aimed to investigate the differential changes in metabolites in the cecal contents of chickens infected with Eimeria tenella using non-targeted metabolomics techniques,to provide reference for the identifi‐cation and determination of potential biomarkers and therapeutic targets for coccidiosis. 【Method】 Sixty healthy Roman pink chickens were randomly divided into a normal group and a model group,with 30 chickens in each group.The model group was orally administered sporulated oocysts of E.tenella（1×10⁴ oocysts/chicken）,while the normal group was orally administered equal volume of normal saline for consecutive 5 d.Then,the experimental chickens were dissected and the chicken cecal contents were aseptically collected.A non-targeted metabolomics analysis was performed on the chicken cecal contents of the normal and model groups to screen for significantly differential metabolites to conduct KEEG metabolic pathway enrichment analysis. 【Result】 A total of 253 significantly differential metabolites were screened from the chicken cecal contents of the model group and the normal group,of which 184 significantly differential metabo‐lites up-regulated expression,including L-tyrosine,L-methionine,L-glutamine,taurine,arachidonic acid,ethyl docosa‐hexaenoate,choline and phosphorylcholine.There were 69 significantly differential metabolites down-regulated expres‐sion,including deoxyadenosine,L-valine,N-（5-aminoamyl） acetamide and N-acetyl-L-glutamate.In the positive ion mode,20 metabolites,including taurine,phosphorylcholine,betaine,L-leucine,L-glutamine,choline,sphingosine and L-tryptophan,were mainly up-regulated.In the negative ion mode,20 metabolites such as arachidonic acid,taurine,L-phenylalanine,L-leucine,succinic acid,xanthine,uracil and L-glutamine were mainly up-regulated.The significant differential metabolites in chicken cecal contents were mainly enriched in 20 KEEG metabolic pathways,such as ABC transporter,aminoacyl-tRNA biosynthesis,lysine degradation,pyrimidine metabolism,purine metabolism and unsatu‐rated fatty acid biosynthesis. 【Conclusion】 After infection with E.tenella,the metabolites of the chicken cecum change significantly,primarily affecting metabolic pathways such as ABC transporter,aminoacyl-tRNA biosynthesis,amino acid metabolism,pyrimidine metabolism and purine metabolism.Therefore,significant differential metabolites such as taurine,phosphorylcholine,L-glutamine and arachidonic acid can be used as potential biomarkers for the diagnosis of coccidiosis in chickens.