海南五指山产区不同雪茄品种晾制后烟叶感官质量和代谢组的差异分析

Differential analysis of sensory quality and metabolomics of tobacco leaves of different cigar varieties after air-curing in Wuzhishan area of Hainan

  • 摘要: 【目的】 研究海南五指山产区2个雪茄品种晾制后烟叶质量差异的物质基础,为今后雪茄品种选育、原料生产技术改进和质量特色彰显提供理论支撑。【方法】 基于高效液相色谱串联质谱(HPLC-MS/MS)、非靶向代谢组学方法和四川中烟工业有限责任公司的感官质量评价方法,分析五指山产区雪茄品种海研101和海研201晾制后烟叶中代谢产物、代谢通路、常规化学成分及感官质量的差异。【结果】 2个雪茄品种晾制后烟叶中总氮、总糖、还原糖、烟碱、钾、氯等常规指标差异较小,但晾制后海研201烟叶的总多酚含量(2.54%)显著高于海研101(P<0.05,下同),海研201烟叶整体颜色转化更深且均匀、外观品质明显更好;感官质量评价显示,海研201烟叶的香韵、香气丰富度、杂气和品质特征等方面均优于海研101,整体感官质量差异较大;2个雪茄品种晾制后烟叶中共检测到1138种代谢物,其中404种代谢物的含量存在显著差异,在HMDB数据库中成功注释到分类及功能的差异代谢物有133种,包括80种上调差异代谢物和53种下调差异代谢物;晾制后烟叶中差异代谢物主要富集在苯丙烷的生物合成与黄酮和黄酮醇的生物合成途径,其中富集到的极显著(P<0.0001)差异代谢物包含莨菪亭、咖啡酸、阿魏酸和对羟基肉桂酸等多酚类物质,以及山柰酚、槲皮素、阿福豆苷、山柰酚3-O-半乳糖苷等黄酮类产物。【结论】 同一生态条件下,2个雪茄品种晾制后烟叶的感官质量和代谢谱存在显著差异。特别是部分多酚和黄酮类代谢产物的相对含量,可能与2个品种雪茄烟叶感官质量的差异有一定关系。

     

    Abstract: 【Objective】 This study aimed to explore the material basis for the quality differences in tobacco leaves of two cigar varieties after air-curing in Wuzhishan area of Hainan, providing theoretical support for the future selection and breeding of cigar varieties, improvements in raw material production techniques and enhancement of quality characteristics. 【Method】 Based on high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS), the nontargeted metabonomics method and the sensory quality evaluation method of China Tobacco Sichuan Industrial Co., Ltd., the differences in metabolites, metabolic pathways conventional chemical component and sensory quality in the tobacco leaves of cigar varieties Haiyan 101 and Haiyan 201 after air-curing in Wuzhishan area were analyzed. 【Result】 The differences in the conventional indexes such as total nitrogen, total sugar, reducing sugar, nicotine, potassium and chlorine in air-cured tobacco leaves of the two cigar varieties were minor. However, after air curing, the total polyphenol content in Haiyan 201 leaves was 2.54%, significantly higher than that of Haiyan 101(P<0.05, the same below). In addition, the overall color transformation of Haiyan 201 tobacco leaves was darker and more uniform, with notably better appearance quality. The sensory quality evaluation showed that Haiyan 201 tobacco leaves were superior to Haiyan 101 in terms of aroma, aroma richness, miscellaneous gas and quality characteristics, with notable differences in the overall sensory quality. A total of 1138 metabolites were detected in air-cured tobacco leaves of the two cigar varieties, with 404 metabolites showing significant differences in content. In the HMDB database, 133 differential metabolites were successfully annotated for classification and function, including 80 up-regulated differential metabolites and 53 down-regulated differential metabolites. The differential metabolites in air-cured tobacco leaves were mainly enriched in phenylpropanoid biosynthesis and flavone and flavonol biosynthesis pathways, with extremely significant(P<0.0001) differential metabolites including the polyphenols such as scopoletin, caffeic acid, ferulic acid and p-coumaric acid, as well as flavonoids such as kaempferol, quercetin, kaempferin and trifolin. 【Conclusion】 The sensory quality and metabolic profiles of air-cured tobacco leaves of two cigar varieties are significantly different under the same ecological conditions. In particular, the relative contents of certain polyphenols and flavonoid metabolites may be related to the formation of sensory quality differences between the cigar tobacco leaves of two cigar varieties.

     

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