7份紫稻品种的褐飞虱抗性特征分析

Brown planthopper resistance characteristic of seven purple rice varieties

  • 摘要: 【目的】探讨紫稻品种的褐飞虱抗性特征及褐飞虱胁迫对紫稻花青素含量的影响,为紫稻抗褐飞虱新品种培育提供参考。【方法】以7份紫稻品种50-6、54-3、NY14、NY17、NY30、WD098和DT01为试验材料,以感性且无紫色表型品种9311为对照,考察紫稻品种的紫色叶鞘表型;参考水稻品种抗褐飞虱鉴定评价标准对7份紫稻品种进行幼苗期和分蘖期抗性评价;利用褐飞虱寄主选择试验和存活试验探索抗性紫稻品种的抗性机制;利用盐酸乙醇溶液避光浸提水稻叶鞘的花青素,用分光光度计测量其吸光度并按照Greey公式计算花青素光密度值和含量;进一步对紫稻品种抗性值与花青素的相对含量进行相关分析。【结果】幼苗期和分蘖期抗性鉴定结果表明,紫稻品种50-6和54-3表现高感,NY14、NY17、NY30和DT01表现抗或高抗,而WD098在幼苗期表现中抗、分蘖期表现抗。虫体存活率和宿主选择性试验结果表明,与感性对照9311相比,NY14和NY30具有显著的抗生性和趋避性(P<0.05,下同),而品种NY17、WD098和DT01的抗生性和趋避性不明显。褐飞虱取食幼苗期和分蘖期的水稻植株后,5份抗性紫稻品种NY14、NY17、NY30、DT01和WD098的花青素含量升高,接虫8 d后仍高于本底水平,而感性品种在接虫4 d后低于本底水平。相关分析结果显示,7份紫稻品种幼苗期和分蘖期抗性值与叶鞘花青素相对含量均呈极显著负相关(P<0.01),褐飞虱取食具有明显抗生性的紫稻品种后存活率与花青素含量和相对含量呈显著负相关。【结论】不同紫稻品种的褐飞虱抗性水平和抗性特征均存在较大差异,幼苗期和分蘖期的褐飞虱抗性值与花青素相对含量呈极显著负相关。

     

    Abstract: 【Objective】The study aimed to preliminarily explore the resistance characteristics of purple rice varieties against brown planthopper(BPH)and the impact of BPH infestation on the anthocyanin content in purple rice,providing reference for the cultivation of new purple rice varieties resistant to BPH.【Method】Seven purple rice varieties,50-6, 54-3,NY14,NY17,NY30,WD098 and DT01,were used as test materials,with the susceptible and non-purple phenotype variety 9311 as the control,to investigate the purple leaf sheath phenotype of purple rice varieties. Resistance evaluation of the seven purple rice varieties at seedling and tillering stages was conducted according to the evaluation standard for BPH resistance of rice varieties. The resistance mechanism of the resistant purple rice varieties was explored through BPH host selection and survival tests,and anthocyanins in rice leaf sheath were extracted by hydrochloric acid-ethanol solution without light. The absorbance was measured by a spectrophotometer,and the anthocyanin optical density and content were calculated according to the Greey formula. Furthermore,a correlation analysis was conducted between the resistance score and anthocyanin relative content of purple rice varieties.【Result】Resistance identification at the seedling and tillering stages revealed that purple rice varieties 50-6 and 54-3 were highly susceptible,while NY14,NY17,NY30,and DT01 exhibited resistance or high resistance against BPH. Variety WD098 showed moderate resistance at the seedling stage and resistance at the tillering stage. The results of the insect survival rate and host selection tests suggested that,compared to the susceptible control 9311,NY14 and NY30 exhibited significant antibiosis and avoidance(P<0.05,the same below), while the antibiosis and avoidance of varieties NY17,WD098,and DT01 were not significant. After BPH feeding at the seedling and tillering stages,the anthocyanin content in five resistant purple rice varieties NY14,NY17,NY30, DT01 and WD098 continued to increase,and still higher than baseline levels after 8 d of infestation,but it fell below baseline levels after a 4-d infestation in susceptible varieties. Correlation analysis indicated that there was an extremely significant negative correlation between the resistance grade and the changes of anthocyanin relative content in the leaf sheaths of 7 purple rice varieties at seedling and tillering stages(P<0.01). The survival rate of BPH after feeding on the resistant purple rice varieties was significantly negatively correlated with the anthocyanin content and anthocyanin relative content.【Conclusion】In summary,there are great differences in the resistance levels and characteristics of BPH among different purple rice varieties,but the BPH resistance scores at seedling and tillering stages show extremely significant negative correlation with the anthocyanin relative content

     

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