泡桐卷野螟(草螟科:斑野螟亚科)线粒体全基因组测序与分析

Sequencing and analysis of the complete mitochondrial genome of Pycnarmon cribrata(Fabricius,1794)(Crambidae: Spilomelinae)

  • 摘要: 【目的】明确泡桐卷野螟[Pycnarmon cribrata(Fabricius,1794)]的线粒体基因组结构特征,分析其系统发育关系,为揭示泡桐卷野螟的分类地位及进化关系研究提供分子依据。【方法】利用Illumina Nova 6000高通量测序平台测定泡桐卷野螟的线粒体基因组,对基因组序列进行注释和分析;基于草螟科8个亚科21种昆虫及2种夜蛾总科昆虫的线粒体蛋白编码基因(Protein-coding genes,PCGs),采用最大似然法和贝叶斯法构建系统发育进化树。【结果】泡桐卷野螟线粒体基因组为双链闭合环状结构,全长15125 bp,共编码37个基因(13个PCGs、22个tRNA基因和2个rRNA基因)和1个A+T富含区,共有14个基因间隔区,10个基因重叠区。泡桐卷野螟线粒体全基因组序列中AT碱基的含量为81.1%,表现出明显的AT偏好性。13个PCGs中除cox1基因以TTG为起始密码子外,其余均以ATN作为起始密码子;除cox1cox2基因以不完全密码子T作为终止密码子外,其余均以TAA结尾。在tRNA二级结构中,trnS1基因缺少DHU臂和DHU环,trnT基因缺少TφC环,并存在U-U和G-U碱基错配现象。系统发育分析结果显示,斑野螟亚科的所有物种聚在同一分支,支持该亚科的单系性;泡桐卷野螟与卷野螟属的乳翅卷野螟(P. lactiferalis)和豹纹卷野螟(P. pantherata)聚为一个分支,构成姐妹群关系。【结论】获得了泡桐卷野螟的线粒体全基因组序列,其线粒体基因组符合草螟科线粒体基因组的一般特征。

     

    Abstract: 【Objective】The study aimed to determine the mitochondrial genome structure characteristics of Pycnarmon cribrata(Fabricius,1794)and analyze its phylogenetic relationship,providing a molecular basis for the taxonomic status and evolutionary relationship of P. cribrata.【Method】The mitochondrial genome of P. cribrata was sequenced by using Illumina Nova 6000 high-throughput sequencing technique,and its genome sequences were annotated and analyzed. Phylogenetic trees were constructed using both maximum likelihood and Bayesian inference methods,based on the mitochondrial protein-coding genes(PCGs)of 21 insect species from 8 subfamilies of Crambidae and 2 insect species from Noctuoidea outgroups.【Result】The mitochondrial genome of P. cribrata was closed double-stranded circular structure,with a total length of 15125 bp. It encoded 37 genes(13 PCGs,22 tRNA genes and 2 rRNA genes)and included 1 A+T-rich region,with 14 intergenic regions and 10 gene overlapping regions. The AT base content in the complete mitochondrial genome sequence of of P. cribrata was 81.1%,representing an obvious AT bias. Among the 13 PCGs,except that cox1 gene had TTG as the start codon,all others began with ATN as the start codon. Except for cox1 and cox2 genes,which ended with the incomplete stop codon T,the remaining genes ended with TAA. In the secondary structures of tRNA,the trnS1 gene lacked DHU arm and loop,and trnT gene lacked the TφC loop. Mismatches of U-U and G-U bases were also existed. The phylogenetic analysis indicated that all species of Spilomelinae belonged to the same branch,confirming Spilomelinae to be a monophyly. P. cribrata,along with P. lactiferalis and P. pantherata from Pycnarmon,gathered into one subgroup,constituting a sister group relationship.【Conclusion】In this study,the complete mitochondrial genome sequence of P. cribrata is obtained. The mitochondrial genome of P. cribrata conforms to the general characteristics of Crambidae mitochondrial genome.

     

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