广西小花老鼠簕可培养细菌多样性及促生、防病、产酶活性分析

Diversity of culturable endophytic bacteria in Acanthus ebracteatus of Guangxi and their growth-promoting, biocontrol effect and enzyme production activity

  • 摘要: 【目的】分析广西小花老鼠簕可培养细菌多样性及促生、防病、产酶活性,为小花老鼠簕种群恢复及筛选可利用菌种资源提供参考依据。【方法】采用稀释涂布法对小花老鼠簕根、茎、叶及根际土壤可培养细菌进行分离、纯化;对菌株的16S rRNA序列进行PCR扩增,测序结果经EzBioCloud比对确定菌株的分类地位;通过功能培养基对菌株的促生溶磷、固氮、产吲哚乙酸(IAA)和分泌铁载体、防病(香蕉枯萎病)及产酶(蛋白酶、淀粉酶和纤维素酶)活性进行分析。【结果】从小花老鼠簕根、茎、叶及根际土壤共筛选获得细菌159株,根据菌落形态及16S rRNA测序结果排除重复后为65株,隶属于8目7科14属,其中芽孢杆菌属(Bacillus)为优势菌属,占分离菌株的64.62%,同时发现1株潜在新种(Gxun-T66),与已发现菌株相似性仅为98.49%;植物促生防病活性结果显示,溶磷菌株34株、固氮菌株31株、产IAA菌株20株、分泌铁载体菌株17株,其中北澳伯克霍尔德氏菌(Burkholderia territorii)Gxun-T33的溶磷和分泌铁载体能力最强;29株对香蕉枯萎病尖孢镰刀菌(Fusarium oxysporum f. sp. Cubense)有明显抑制作用,其中暹罗芽孢杆菌(Bacillus siamensis)Gxun-T25的抑菌效果最好,抑菌率达69.67%;产酶活性结果显示,产蛋白酶菌株56株,产淀粉酶49株,产纤维素酶48株,同时产3种酶活性的菌株有34株,占分离菌株的52.31%。【结论】红树植物小花老鼠簕富含微生物资源且具备促生、防病及产酶活性,同时发现1株潜在新种。

     

    Abstract: 【Objective】The aim was to analyze the diversity of culturable bacteria of Acanthus ebracteatus in Guangxi and their growth-promoting, biocontrol effect and enzyme production activity, so as to provide reference for the recovery of A. ebracteatus ilicifolius population and the screening of available strain resources. 【Method】The culturable bacteria in the roots,stems,leaves and rhizosphere soil were isolated and purified by dilution coating method. The 16S rRNA sequence of the strain was amplified by PCR, and the sequencing results were compared by EzBioCloud to determine the taxonomic status of the strain. The growth-promoting phosphate solubilization, nitrogen fixation, indoleacetic acid(IAA) production and siderophore secretion,disease prevention(banana fusarium wilt) and enzyme production(protease, amylase and cellulase) activities of the strains were analyzed by functional medium. 【Result】A total of 159 strains of bacteria were screened from the roots,stems, leaves and rhizosphere soil of A. ebracteatus. According to the colony morphology and the 16S rRNA sequencing results, 65 strains were excluded after duplication, belonging to 8 orders,7 families and 14 genera. Among them, Bacillus was the dominant genus, accounting for 64.62 % of the isolated strains. At the same time, a potential new species(Gxun-T66) was found, and the similarity with the discovered strains was only 98.49%. The results of plant growth-promoting and biocontrol effect showed that 34 strains were phosphorus-solubilizing strains,31 strains were nitrogen-fixing strains,20 strains were IAA-producing strains and 17 strains were siderophoresecreting strains. Among them,strain Burkholderia territorii Gxun-T33 had the strongest ability to dissolve phosphorus and to secrete siderophore; 29 strains had obvious inhibition effect on Fusarium oxysporum f. sp. Cubense,among which strain Bacillus siamensis Gxun-T25 had the best inhibition effect, with an inhibition rate of 69.67%. The results of the enzyme activity showed that there were 56 protease-producing strains,49 amylase-producing strains,48 cellulase-producing strains and 34 strains producing three kinds of enzyme activities at the same time, accounting for 52.31% of the isolated strains. 【Conclusion】The mangrove plant A. ebracteatus is rich in microbial resources and has the activities of promoting growth, biocontrol effect and enzyme production. At the same time, a potential new species has been.

     

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