多酚处理对甜樱桃采后品质和活性氧代谢的影响

Effects of polyphenol treatment on postharvest quality and reactive oxygen species metabolism in Prunus avium L.

  • 摘要: 【目的】探究室温条件下多酚处理对甜樱桃采后品质和活性氧(ROS)代谢的影响,以期为保持甜樱桃果实品质和延长货架期提供新途径。【方法】选用4种多酚单体酚(咖啡酸、儿茶素、绿原酸和原花青素)处理甜樱桃,以去离子水处理作为对照组,测定在20℃室温贮藏过程中(0~10 d)甜樱桃果实品质、ROS、非酶抗氧化剂含量、抗氧化酶活性及细胞膜脂质过氧化等指标的变化,并对多酚处理后甜樱桃品质与抗氧化系统相关特征进行相关分析。【结果】与对照组相比,多酚处理组甜樱桃果实的失重率明显降低,多酚处理降低甜樱桃贮藏过程中的呼吸速率,减少果实内部营养物质的过度消耗,保持可滴定酸(TA)和维生素C(Vc)较高含量,表现出更高水平的抗氧化酶活性、花青素、总酚、总黄酮和抗氧化能力,同时抑制超氧阴离子(O2-·)的产生和积累,并减轻果实细胞膜的脂质过氧化程度。4种多酚单体酚中,原花青素的处理效果最佳。当贮藏至第10 d时,原花青素处理的甜樱桃果实失重率和腐烂率最低,分别为1.36%和3.49%,Vc含量仅下降35.36%,花青素含量为0.24 g/kg,显著高于对照组(P<0.05,下同),总抗氧化能力较对照组显著增加62.61%,丙二醛(MDA)含量仅增加52.45%。果实失重率与腐烂率、MDA含量和O2-·产生率呈极显著正相关(P<0.01,下同),与Vc含量、非酶抗氧化剂(总酚、总黄酮、花青素)含量和总抗氧化能力呈极显著负相关,与TA含量呈显著负相关。【结论】多酚处理可抑制ROS积累引起的甜樱桃果实氧化衰老,有效保持果实营养价值并延长其货架期;以原花青素处理的保鲜效果最佳,保持甜樱桃细胞膜结构的完整性,有效防止抗氧化能力下降,延缓果实衰老。

     

    Abstract: 【Objectives】This study aimed to investigate the effects of polyphenol treatment on the postharvest quality and reactive oxygen species(ROS) metabolism of sweet cherries under room temperature conditions,in order to provide a new way to maintain the quality of sweet cherry fruit and extend the shelf life. 【Method】The experiment utilized four monomeric polyphenols(caffeic acid,catechin,chlorogenic acid and procyanidin) to treat sweet cherries,with deionized water treatment as the control group. Changes in sweet cherry quality,ROS,non-enzymatic antioxidants content,antioxidant enzymes activity,and cellular membrane lipid peroxidation during storage at 20 ℃(0-10 d) were measured. Additionally,a correlation analysis was conducted between the quality of sweet cherries treated with polyphenols and the characteristics of the antioxidant system. 【Result】Compared to the control group,the treated sweet cherries exhibited a greatly reduced weight loss rate. Polyphenol treatment decreased the respiration rate during storage,reduced the excessive consumption of internal nutrients,maintained higher levels of titrable acid(TA) and vitamin C(Vc),and demonstrated increased antioxidant enzyme activity,anthocyanins,total phenols,total flavonoids,and antioxidant capacity. It also inhibited the production and accumulation of superoxide anions(O2-·) and mitigated the degree of lipid peroxidation in sweet cherry cell membranes. Among the four monomeric polyphenols,procyanidin showed the best treatment effect. On the 10th day of storage,sweet cherries treated with procyanidin had the lowest weight loss rate(1.36%) and decay rate(3.49%),a decrease in Vc content of only 35.36%,an anthocyanin content of 0.24 g/kg,significantly higher than control group(P<0.05,the same below),total antioxidant capacitr significantly increased by 62.61% compared to control group, and only a 52.45% increase in malondialdehyde(MDA) content. Weight loss rate was extremely significantly positively correlated with decay rate,MDA content and O2-· production rate(P<0.01, the same below),and extremely significantly negatively correlated with Vc content,non-enzymatic antioxidants(total phenols, total flavonoids, anthocyanins) content and total antioxidant capacity,and significantly negatively correlated with TA content. 【Conclusion】Polyphenol treatment can inhibit oxidative senescence in sweet cherries caused by ROS accumulation,effectively preserve the nutritional value of the fruit,and extend its shelf life. The best preservation effect is achieved by treating with anthocyanins, thereby maintaining the integrity of the cellular membrane structure,effectively preventing the decline in antioxidant capacity,and delaying fruit senescence.

     

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