Physiological responses of different varieties of soybean to low phosphorus stress and comprehensive evaluation
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摘要: 【目的】研究不同大豆品种幼苗对低磷胁迫的响应,为耐低磷大豆的筛选和育种改良提供理论依据。【方法】以13个大豆品种[黔豆11号(Qd11)、商豆1013(Sd)、牛毛黄豆(Nm)、中黄13号(Zh13)、七星1号(Qx1)、华春6号(Hc6)、中黄30(Zh30)、华夏3号(Hx3)、矮选(Ax)、华夏2号(Hx2)、绥阳豆(Sy)、中黄301(Zh301)和中黄61(Zh61)]为试验材料,设正常供磷(0.5 mmol/L KH2PO4)和低磷胁迫(0.02 mmol/L KH2PO4)2个磷处理,进行沙培试验,研究低磷胁迫下不同大豆品种幼苗的生长形态和生理特性,并通过隶属函数法综合评价不同大豆品种的耐低磷能力。【结果】低磷胁迫下,大豆的株高、茎粗、叶面积、地上部干重和磷含量有降低趋势,而根冠比和地上部磷利用效率有上升趋势;低磷胁迫也对大豆叶片的SPAD值和荧光参数产生影响,SPAD值、最大荧光产量(Fm)、可变荧光(Fv)、PSⅡ潜在活性(Fv/Fo)、PSⅡ最大光化学效率(Fv/Fm)多为降低趋势,而初始荧光(Fo)多为升高趋势;此外,低磷胁迫下大豆叶片丙二醛(MDA)含量及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性有上升趋势。基于耐低磷能力综合值(D),可将13个大豆品种分为耐低磷型、中间型和低磷敏感型,其中耐低磷型品种(Qx1、Sy和Hx2)整体表现为生理调节能力较强,有较高的地上部磷利用效率,能通过提高抗氧化酶活性降低叶片损伤,维持正常光系统途径;中间型品种(Ax、Zh30、Hc6和Qd11)叶片膜损伤较低,但光系统受损较大;低磷敏感类型大豆品种(Zh301、Hx3、Zh61、Zh13、Sd和Nm)地上部磷利用效率降低,生理调节能力较弱,叶片功能受损严重。【结论】不同大豆品种对低磷胁迫的响应程度存在差异,参试材料中Qx1、Sy和Hx2的耐低磷能力相对较强,能更好适应低磷环境来保证正常的生长发育和生理活动。Abstract: 【Objective】To investigate how seedlings of various soybean varieties reacted to low phosphorus stress and to offer a theoretical reference for selecting and breeding more low phosphorus tolerant soybeans.【Method】Thirteen soybean varieties Qiandou 11 (Qd11),Shangdou 1013 (Sd),Niumao soybean (Nm),Zhonghuang 13 (Zh13),Qixing 1(Qx1),Huachun 6 (Hc6),Zhonghuang 30 (Zh30),Huaxia 3 (Hx3),Aixuan(Ax),Huaxia 2 (Hx2),Suiyangdou(Sy),Zhonghuang 301 (Zh301),and Zhonghuang 61 (Zh61) were used as test materials.Two phosphorus treatments were set up:0.5 mmol/L KH2PO4 for normal phosphorus supply and 0.02 mmol/L KH2PO4 for low phosphorus stress.The effects of low phosphorus stress on the growth morphology and physiological characteristics of soybean seedlings were investigated through the sand cultivation test.The ability of different soybean varieties to tolerate low phosphorus was comprehensively evaluated by the membership function method.【Result】While the root-shoot ratio and aboveground phosphorus use efficiency tended to rise,soybean plant height,stem diameter,leaf area,aboveground dry weight,and phospho-rus content tended to decrease under low phosphorus stress.The SPAD values and fluorescence parameters of soybean leaves were also impacted by low phosphorus stress;there were primarily increasing trends in initial fluorescence (Fo) and decreasing trends in maximum fluorescence yield (Fm),variable fluorescence (Fv),potential PS II activity (Fv/Fo),and maximum photochemical efficiency of PSII (Fv/Fm).Additionally,low phosphorus stress led to a tendency of decreasing the malonaldehyde(MDA) content of soybean leaves,while superoxide dismutase (SOD) and peroxidase (POD) activities exhibited an increasing trend.The thirteen soybean varieties could be divided into three categories based on capacity comprehensive value (D) of the comprehensive evaluation of low phosphorus tolerance:low phosphorus tolerant type,intermediate type,and low phosphorus sensitive type.Low phosphorus tolerant varieties (Qx1,Sy and Hx2) demonstrated greater physiological regulation overall,higher aboveground phosphorus utilization efficiency,and the ability to maintain normal photosystem pathways by increasing the activity of antioxidant enzymes to reduce leaf damage;inter-mediate varieties (Ax,Zh30,Hc6 and Qd11) had lower leaf membrane damage but greater photosystem damage;low phosphorus sensitive soybean varieties (Zh301,Hx3,Zh61,Zh13,Sd and Nm) had reduced aboveground phosphorus utilization efficiency and weak physiological regulation ability,their leaf functions were severely damaged.【Conclusion】Different soybean varieties varied in their response to low phosphorus stress.Among the materials used,Qx1,Sy and Hx2 are comparatively more tolerant of low phosphorus and better suited to low phosphorus environments to ensure normal growth,development and physiological activities.
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敖雪，谢甫绨，张惠君，刘婧琦. 2009. 磷对不同磷效率基因型大豆保护酶的影响［J］. 大豆科学，28（1）：67-71.［Ao X，Xie F T，Zhang H J，Liu J Q. 2009. Effect of phosphorus on protective enzymes of soybean cultivars with different phosphorus efficiencies ［J］. Soybean Science，28（1）：67-71.］ 鲍士旦 . 2000. 土壤农化分析［M］. 北京：中国农业出版社 .［Bao S D. 2000. Soil agrochemical analysis［M］. Beijing：China Agriculture Press.］ 蔡丽平，吴鹏飞，侯晓龙，马祥庆，江硕，任晶晶. 2012. 磷胁迫对水土保持先锋植物类芦光合特性的影响［J］. 水土保持学报，26（6）：281-285.［Cai L P，Wu P F，Hou X L，Ma X Q，Jiang S，Ren J J. 2012. Effects of phosphorus stress on photosynthetic characteristics of pioneer plant Neyraudia reynaudiana on soil and water conservation［J］. Journal of Soil and Water Conservation，26（6）：281-285.］doi：10. 13870/j.cnki.stbcxb.2012.06.001. 蔡银美，赵庆霞，张成富. 2021. 低磷下植物根系分泌物对土壤磷转化的影响研究进展［J］. 东北农业大学学报，52（2）：79-86.［Cai Y M，Zhao Q X，Zhang C F. 2021. Effect of plant root exudates on soil phosphorus transformation under low phosphorus：A review［J］. Journal of Northeast Agricultural University，52（2）：79-86.］doi：10.19720/j.cnki.issn.1005-9369.2021.02.10. 陈国兴. 2017. 磷素营养水平对大豆光合作用及磷素吸收积累的影响［D］. 哈尔滨：东北农业大学.［Chen G X. 2017.Effects of phosphorus nutrition on photosynthesis and phosphorus absorption and accumulation in soybean［D］.Harbin：Northeast Agricultural University.］ 陈健晓，王小娟，屠乃美，王效宁，唐清杰，刘爱玉. 2021. 低磷胁迫对4种香稻苗期磷积累量及叶绿素荧光参数的影响［J］. 湖南农业大学学报（自然科学版），47（4）：369-377.［Chen J X，Wang X J，Tu N M，Wang X N，Tang Q J，Liu A Y. 2021. Effects of low phosphorus stress on phosphorus accumulation and chlorophyll fluorescence of four scented rice materials at the seedling stage［J］. Journal of Hunan Agricultural University（Natural Sciences），47（4）：369-377.］doi：10.13331/j.cnki.jhau.2021.04.001. 崔博文，乔光，范付华，丁贵杰，文晓鹏. 2017. 不同种源马尾松种质耐低磷的主成分与灰色关联度分析［J］. 西南大学学报（自然科学版），39（8）：49-56.［Cui B W，Qiao G，Fan F H，Ding G J，Wen X P. 2017. Principal component analysis and grey correlation analysis for low phosphorus tolerance evaluation in masson pine（Pinus massoniana）of different provenances［J］. Journal of Southwest University（Natural Science Edition），39（8）：49-56.］doi：10.13718/j.cnki.xdzk.2017.08.007. 董秋平，赵恢，张小芳，张炜坤，张华雪，张锴，李桂兰，乔亚科. 2017. 低磷胁迫下不同野生大豆的形态和生理响应差异［J］. 江苏农业科学，45（9）：79-83.［Dong Q P，Zhao H，Zhang X F，Zhang W K，Zhang H X，Zhang K，Li G L，Qiao Y K. 2017. Differences in morphological and physiological responses of different wild soybeans under low phosphorus stress［J］. Jiangsu Agricultural Sciences，45（9）： 79-83.］doi：10.15889/j.issn.1002-1302.2017.09.021. 房福力，姚志鹏，林伟，李玉中，徐春英，李巧珍. 2015. 柠檬酸对温室土壤磷有效性的影响［J］. 农业环境科学学报，34（6）：1166-1173.［Fang F L，Yao Z P，Lin W，Li Y Z，Xu C Y，Li Q Z. 2015. Effects of citric acid on phosphorus availability for soil in greenhouse field［J］. Journal of AgroEnviron-ment Science，34（6）：1166-1173.］doi：10.11654/jaes.2015.06.021. 龚丝雨，梁喜欢，杨帅强，张世川，朱肖文，刘齐元. 2019. 低磷胁迫对不同磷效率基因型烟草苗期生长及生理特征的影响［J］. 核农学报，33（6）：1217-1224.［Gong S Y，Liang X H，Yang S Q，Zhang S C，Zhu X W，Liu Q Y. 2019.Effect on growth and physiological characteristics of tobacco genotypes with different P-efficiency at seedling stage under low-phosphorus stress［J］. Journal of Nuclear Agricultural Sciences，33（6）：1217-1224.］doi：10.11869/j.issn.100-8551.2019.06.1217. 何榜眼，刘世男，杨梅，梁喜献. 2022. 低磷胁迫对大花序桉幼苗叶片生理指标的影响［J］. 西南农业学报，35（2）：418-424.［He B Y，Liu S N，Yang M，Liang X X. 2022. Effects of low phosphorus stress on physiological characteristics of Eucalyptus coleziana seedling leaves［J］. Southwest China Journal of Agricultural Sciences，35（2）：418-424.］doi：10.16213/j.cnki.scjas.2022.2.023. 侯宪斌，易强，滕峥，杨翠凤，孔宇，刘应红，李玉峰. 2023. 不同玉米种质花期对干旱和高温胁迫的响应［J］. 南方农业学报，54（6）：1598-1611.［Hou X B，Yi Q，Teng Z，Yang C F，Kong Y，Liu Y H，Li Y F. 2023. Response of different maize germplasms to drought stress and heat stress at anthesis period［J］. Journal of Southern Agriculture，54（6）： 1598-1611.］doi：10.3969/j.issn.2095-1191.2023.06.002. 柯野，谢璐，蓝林，唐新莲. 2019. 低磷胁迫对甘蔗幼苗生长和生理特性的影响［J］. 江苏农业科学，47（20）：114-118.［Ke Y，Xie L，Lan L，Tang X L. 2019. Effects of low phos‐ phorus stress on growth and physiological characteristics of sugarcane seedlings［J］. Jiangsu Agricultural Sciences， 47（20）：114-118.］doi：10.15889/j.issn.1002-1302.2019.20. 025.
李华丽. 2011. 不同生育期烤烟翠碧一号应对磷胁迫的生理响应［D］. 福州：福建农林大学 .［Li H L. 2011. Physiological responses of flue-cured tobacco（CuiBi 1）to phosphorus stress at different stages［D］. Fuzhou：Fujian Agriculture and Forestry University.］ 李键，黄锦湖，洪滔，吴承祯，洪伟. 2013. 低磷胁迫对雷公藤幼苗叶片生理生化特性的影响［J］. 植物科学学报，31（3）：286-296.［Li J，Huang J H，Hong T，Wu C Z，Hong W. 2013. Effects of low phosphorus stress on leaf physiological and biochemical characteristics of Tripterygium wilfordii Hook. f. seedlings［J］. Plant Science Journal，31（3）：286-296.］doi：10.3724/SP.J.1142.2013.30286. 廖红，李欣欣. 2018. 一种大豆水培营养液：CN201810570975.6［P/OL］. 2018-09-21［2023-09-08］. https://kns.cnki.net/kcms2/article/abstract" target="_blank"> https://kns.cnki.net/kcms2/article/abstract？v=C_qHzaiysLWc4Y08F3jVlfiGDuO05rPDEViajlOU_Pg9TUrp8RPNpBKw9e5atSb4EaNrcIsiuFSy3JW5NJ0jcUj2tnibTB_zKl49oEd4QjMEhD5jE7KL1r-k8ki ZKZZeQfyVIwaPMGA=&uniplatform=NZKPT&language=CHS.［Liao H，Li X X. 2018. A soybean hydroponic nutrient solution：CN201810570975.6［P/OL］. 2018-09-21［2023-09-08］. https://kns.cnki.net/kcms2/article/abstract" target="_blank"> https://kns.cnki.net/kcms2/article/abstract？v=C_qHzaiysLWc4Y08F3jVlfiGDuO05rPDEViajlOU_Pg9TUrp8RPNpBKw9e5atSb4EaNrcIsiuFSy3JW5NJ0jcUj2tnibTB_zKl49oEd4QjMEhD5jE7KL1r-k8kiZKZZeQfyVIwaPMGA=&uniplatform=NZKPT&language=CHS.］ 刘渊，李喜焕，王瑞霞，张彩英. 2015. 大豆耐低磷指标筛选与耐低磷品种鉴定［J］. 中国农业科技导报，17（4）：30-41.［Liu Y，Li X H，Wang R X，Zhang C Y. 2015. Screen indexes for soybean tolerance to phosphorus deficiency and identification of low phosphorus tolerant soybean cultivars［J］. Journal of Agricultural Science and Technology， 17（4）：30-41.］doi：10.13304/j.nykjdb.2015.349. 罗红艳，费裕翀，曹光球，陈潇潇，刘丽，叶义全. 2018. 低磷和铝胁迫对杉木光合及叶绿素荧光特性的影响［J］. 亚热带农业研究，14（4）：229-235.［Luo H Y，Fei Y C，Cao G Q，Chen X X，Liu L，Ye Y Q. 2018. Effect of low phosphorus and aluminum stress on photosynthesis and chlorophyll fluorescence characteristics of chinese fir（Cunninghamia）［J］. Subtropical Agriculture Research，14（4）：229-235.］doi：10.13321/j.cnki.subtrop.agric.res.2018.04.003. 秦成，裴红宾，张永清，连慧达. 2015. 磷素对小豆幼苗生长发育的影响［J］. 作物杂志，（3）：122-129.［Qin C，Pei H B，Zhang Y Q，Lian H D. 2015. Effects of phosphorus on the growth and development of adzuki bean at seedling stage［J］. Crops，（3）：122-129.］doi：10.16035/j.issn.1001-7283. 2015.03.023. 孙小玲，许岳飞，马鲁沂，周禾. 2010. 植株叶片的光合色素构成对遮阴的响应［J］. 植物生态学报，34（8）：989-999.［Sun X L，Xu Y F，Ma L Y，Zhou H. 2010. A review of acclimation of photosynthetic pigment composition in plant leaves to shade environment［J］. Chinese Journal of Plant Ecology，34（8）：989-999.］doi：10.3773/j.issn.1005-264x.2010.08.012. 王敏，张从宇，马同富，姚维传 . 2004. 大豆品种苗期抗旱性研究［J］. 中国油料作物学报，26（3）：30-33.［Wang M，Zhang C Y，Ma T F，Yao W C. 2004. Studies on the drought resistance of seedling in soybean［J］. Chinese Journal of Oil Crop Sciences，26（3）：30-33.］doi：10.3321/j. issn：1007-9084.2004.03.007. 王学奎. 2006. 植物生理生化实验原理和技术［M］. 北京：高等教育出版社 .［Wang X K. 2006. Principle and technology of plant physiological and biochemical experiment［M］. Beijing：Higher Education Press.］ 武海燕，李喜焕，李文龙，孔佑宾，杜汇，张彩英. 2020. 大豆耐低磷性状鉴定及优异种质筛选［J］. 河南农业科学，49（1）：61-67.［Wu H Y，Li X H，Li W L，Kong Y B，Du H，Zhang C Y. 2020. Identification of low phosphorus tolerant traits and selection of elite genotypes in soybean［J］. Journal of Henan Agricultural Sciences，49（1）：61-67.］doi：10. 15933/j.cnki.1004-3268.2020.01.008. 武兆云. 2014. 大豆苗期耐低磷性状评价和低磷胁迫的分子机理初步研究［D］. 南京：南京农业大学.［Wu Z Y. 2014.Evaluation of low phosphate tolerance at seedling stage and molecular essential study on phosphate stress in soybean［D］. Nanjing：Nanjing Agricultural University.］ 解斌，安秀红，陈艳辉，程存刚，康国栋，周江涛，赵德英，李壮，张艳珍，杨安. 2022. 不同苹果砧木对持续低磷的响应及适应性评价［J］. 中国农业科学，55（13）：2598-2612.［Xie B，An X H，Chen Y H，Cheng C G，Kang G D，Zhou J T，Zhao D Y，Li Z，Zhang Y Z，Yang A. 2022. Response and adaptability evaluation of different apple rootstocks to continuous phosphorus deficiency［J］. Scientia Agricultura Sinica，55（13）：2598-2612.］doi：10.3864/j.issn.0578-1752. 2022.13.010. 杨春婷，张永清，马星星，陈伟，董璐，张楚，路之娟. 2018. 苦荞耐低磷基因型筛选及评价指标的鉴定［J］. 应用生态学报，29（9）：2997-3007.［Yang C T，Zhang Y Q，Ma X X，Chen W，Dong L，Zhang C，Lu Z J. 2018. Screening genotypes and identifying indicators of different fagopyrum tataricum varieties with low phosphorus tolerance［J］. Chinese Journal of Applied Ecology，29（9）：2997-3007.］doi： 10.13287/j.1001-9332.201809.021. 余利平，张春雷，马霓，李俊，李光明. 2013. 甘蓝型油菜对干旱和低磷双重胁迫的生理反应Ⅱ：叶片叶绿素含量及叶绿素荧光参数［J］. 干旱地区农业研究，31（2）：169-175.［Yu L P，Zhang C L，Ma N，Li J，Li G M. 2013. Physiological responses of rapeseed（Brassica napus）to drought and phosphorus deficiency Ⅱ：Chlorophyll content and chlorophyll fluorescence parameters［J］. Agricultural Research in the Arid Areas，31（2）：169-175.］doi：10.3969/j.issn. 1000-7601.2013.02.031. 原梦，赵峥艳，李雪林，张富厚，刘逢举，孟超敏. 2019. 低磷胁迫下不同基因型棉花生理和形态的响应差异［J］. 种子， 38（4）：20-23.［Yuan M，Zhao Z Y，Li X L，Zhang F H，Liu F J，Meng C M. 2019. Physiology and morphological characteristics among different cotton genotypes in response to low phosphorus stress［J］. Seed，38（4）：20-23.］doi：10.16590/j.cnki.1001-4705.2019.04.020. 袁天佑，王俊忠，冀建华，牛俊义，慕兰. 2017. 长期施肥条件下潮土有效磷的演变及其对磷盈亏的响应［J］. 核农学报，31（1）：125-134.［Yuan T Y，Wang J Z，Ji J H，Niu J Y，Mu L. 2017. Changes in soil available phosphorus and its response to phosphorus balance under long-term fertilization in fluvo-aquic soil［J］. Journal of Nuclear Agricultural Sciences，31（1）：125-134.］doi：10.11869/j.issn.100-8551. 2017.01.0125. 张雪洁，谭晓风，袁军，姜志娜，叶思诚. 2012. 低磷胁迫对油茶叶绿素荧光参数的影响［J］. 经济林研究，30（2）：48-51.［Zhang X J，Tan X F，Yuan J，Jiang Z N，Ye S C. 2012 Effects of low phosphorus stress on chlorophyll fluorescence parameters in Camellia oleifera［J］. Nonwood Forest Research，30（2）：48-51.］doi：10.14067/j.cnki.1003-8981.2012.02.028. 赵坤. 2015. 不同基因型玉米幼苗对低磷胁迫的适应机理［D］. 扬州：扬州大学 .［Zhao K. 2015. The mechanism of adaptation of different genotypes maize seedlings to low phosphorus stress［D］. Yangzhou：Yangzhou University.］ 郑金凤，董少鸣，李成璞，白志英，李存东，毕常锐. 2010a. 低磷胁迫对小麦代换系保护酶活性和丙二醛含量的影响及染色体效应［J］. 植物营养与肥料学报，16（6）：1366-1372.［Zheng J F，Dong S M，Li C P，Bai Z Y，Li C D，Bi C R. 2010a. Effects of phosphorus deficiency stress on protective enzyme activities，MDA content and chromosome of wheat substitution lines［J］. Plant Nutrition and Fertilizers Science，16（6）：1366-1372.］ 郑金凤，李成璞，董少鸣，白志英，李存东，毕常锐. 2010b. 低磷胁迫对小麦代换系叶绿素和类胡萝卜素含量的影响及染色体效应［J］. 华北农学报，25（5）：161-165.［Zheng J F，Li C P，Dong S M，Bai Z Y，Li C D，Bi C R. 2010b.The effect of phosphorus deficiency stress on chlorophyll content and corticoid content and chromosome of wheat substitution lines［J］. Acta Agriculturae Boreali-Sinica，25（5）：161-165.］doi：10.7668/hbnxb.2010.05.033. 郑金凤，米少艳，婧姣姣，白志英，李存东. 2013. 小麦代换系耐低磷生理性状的主成分分析及综合评价［J］. 中国农业科学，46（10）：1984-1993.［Zheng J F，Mi S Y ，Jing J J，Bai Z Y，Li C D. 2013. Principal component analysis and comprehensive evaluation on physiological traits of tolerance to low phosphorus stress in wheat substitution［J］.Scientia Agricultura Sinica，46（10）：1984-1993.］doi：10. 3864/j.issn.0578-1752.2013.10.003. 周哲宇，徐超，胡策，王海湘，梁谢恩，张汝民，温国胜. 2018.毛竹快速生长期的叶绿素荧光参数特征［J］. 浙江农林大学学报，35（1）：75-80.［Zhou Z Y，Xu C，Hu C，Wang H X，Liang X E，Zhang R M，Wen G S. 2018. Chlorophyll fluorescence characteristics of Phyllostachys edulis during its fast growth period［J］. Journal of Zhejiang A&F University，35（1）：75-80.］doi：10.11833/j.issn.2095-0756.2018. 01.010. Maxwell K，Johnson G N. 2000. Chlorophyll fluorescence—A practical guide［J］. Journal of Experimental Botany，51（345）：659-668. doi：10.1093/jxb/51.345.659.
Vengavasi K，Pandey R. 2018. Root exudation potential in contrasting soybean genotypes in response to low soil phosphorus availability is determined by photo-biochemical processes［J］. Plant Physiology and Biochemistry，124：1-9. doi：10.1016/j.plaphy.2018.01.002.
Wu Q，Zhu D Z，Wang C，Ma Z H，Wang J H. 2012. Diagnosis of freezing stress in wheat seedlings using hyperspectral imaging［J］. Biosystems Engineering，112（4）：253-260. doi： 10.1016/j.biosystemseng.2012.04.008.
Zhou J，Xie J N，Liao H，Wang X R. 2014. Overexpression of β-expansin gene GmEXPB2 improves phosphorus efficiency in soybean［J］. Physiologia Plantarum，150（2）：194-204.doi：10.1111/ppl.12077.
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