激素对圆齿野鸦椿种子萌发的影响

Effects of hormones on seed germination of Euscaphis konishii

  • 摘要: 【目的】探究激素在圆齿野鸦椿种子萌发中的作用及其与活性氧的关系,为圆齿野鸦椿种子破眠技术和种苗高效繁育技术提供理论依据。【方法】以自然层积210 d的圆齿野鸦椿种子为试验材料,去除外部硬质种壳,进行为期12 d的萌发试验,测定纯水、不同浓度的赤霉素(GA3)(20、40和60μmol/L)和脱落酸(ABA)(10、20和30μmol/L)处理下萌发率及活性氧、抗氧化物质、植物激素、可溶性糖和可溶性蛋白含量的变化,并观察记录种子的萌发形态。【结果】纯水处理萌发率最终达77.8%,GA3处理萌发率最高可达82.2%,ABA处理萌发率最终均未达50.0%,而保留硬质种皮纯水处理的种子萌发率仅17.8%。3种浓度的GA3处理均可有效缩短圆齿野鸦椿种子萌发率达50.0%的时间(T50),促进根茎生长,但其最终萌发率均与纯水处理无显著差异(P>0.05);ABA处理浓度越高,对圆齿野鸦椿种子抑制萌发作用越强。相关分析显示,在萌发的第8和12 d,萌发率与GA3、油菜素内酯(BR)和茉莉酸(JA)呈显著正相关(P<0.05,下同),且超氧阴离子(O2-·)与BR和JA含量呈显著负相关,与过氧化氢酶(CAT)、ABA、ABA/GA3均呈显著正相关。【结论】添加GA3可有效缩短圆齿野鸦椿种子萌发率达50%的时间,促进根茎生长,O2-·与ABA的积累对种子萌发存在抑制作用,BR和JA对种子萌发的促进作用可能与对O2-·的清除有关。

     

    Abstract: 【Objective】The role of hormones in seed germination of Euscaphis konishii and its relationship with reac-tive oxygen species were explored to provide an important theoretical basis for its seed dormancy breaking technology and efficient seedling breeding technology.【Method】E.konishii seeds with 210 d natural stratified were used as materials,outer hard seed coat was removed,a 12 d germination experiment was conducted.The changes of germination rate,active oxygen,antioxidant substances,plant hormones,soluble sugar and soluble protein contens under pure water,different con-centrations of gibberellin(GA3)(20,40 and 60μmol/L) and abscisic acid(ABA)(10,20 and 30μmol/L)were measured,and germination morphology of seeds was observed.【Result】The germination rate of pure water treatment was 77.8%,the germination rate of GA3 treatment was up to 82.2%,the germination rate of ABA treatment did not reach 50.0%,and the germination rate of pure water treatment with hard seed coat was only 17.8%.The three concentrations of GA3 treat-ments could effectively shorten the time when germination rate reached 50.0%(T50) and promote root and stem growth,but the final germination rate was not significantly different from that of pure water treatment(P>0.05).ABA treatment could effectively inhibit the germination of E.konishii seeds.The higher the concentration of ABA,the stronger the inhibi-tion.Correlation analysis showed that the germination rate was significantly positively correlated with GA3,brassinolide(BR) and jasmonic(JA) at 8 and 12 d(P<0.05,the same below),superoxide anion(O2-·) was negatively correlated with BR and JA contents,and significantly positively correlated with catalase(CAT),ABA,ABA/GA3.【Conclusion】Adding GA3 can effectively shorten the time of the seed germination rate of E.konishii to reach 50%and promote the growth of root and stem.The accumulation of O2-·and ABA inhibit seed germination and the promotion of BR and JA on seed germi-nation in E.konishii may be related to the removal of O2-·.

     

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