广东省大豆农家种芽期耐盐性鉴定

Identification of salt tolerance of soybean landraces at germination stage in Guangdong

  • 摘要: 【目的】鉴定广东省大豆农家种芽期耐盐性,发掘广东省大豆农家种中潜在的优异性状,筛选出耐盐性较强的种质资源,为耐盐品种的改良和培育提供优质亲本材料。【方法】以102份广东农家大豆种质资源为试验材料,用150 mmol/L NaCl进行盐胁迫处理,以蒸馏水为对照,处理后测定发芽势、发芽率、盐害指数、胚轴长、胚根长、鲜重和干重,根据盐害指数进行耐盐性初步分级,计算其余指标相对值并进行相关分析,采用主成分分析法和隶属函数法计算综合评价值D,并进行耐盐性排序,使用聚类分析法筛选耐盐性较强的种质资源。【结果】耐盐性初步分级结果显示,102份种质资源中,高耐种质资源占比最多,较耐种质资源次之,中耐和较敏感种质资源占比相同,无敏感种质资源。与对照相比,盐胁迫下大豆芽期指标均降低,其受抑制程度排序为胚根长>胚轴长>鲜重>干重>发芽势>发芽率,芽期胚根长受抑制作用较大,发芽率受抑制作用较小;相关分析结果显示,多数指标间均存在一定程度的相关性;通过主成分分析得到2个主成分,累积贡献率达88.17%,其中第一主成分(PC1)中特征向量绝对值最大的指标为相对发芽势,第二主成分(PC2)中特征向量绝对值最大的指标为相对发芽率和盐害指数;采用获得的2个主成分作为综合指标,根据D值进行耐盐性排序,排名前5的种质资源编号分别为374、310、358、357和309;通过聚类分析筛选出耐盐性较强的种质资源,编号分别为310、358、374和331。【结论】筛选出4份耐盐性较强的农家大豆种质资源,可作为亲本材料用于耐盐种质创制,或者作为抗盐渍种质资源用于盐渍化土地的开发利用。相对发芽势和相对发芽率指标可用于大豆耐盐性评价与耐盐种质资源筛选。

     

    Abstract: 【Objective】The purpose of the study was to identify the salt tolerance of soybean landraces at germination stage in Guangdong,to discover the potential excellent traits in soybean landraces in Guangdong,to screen out germplasms with strong salt tolerance,and to provide high-quality parent materials for the improvement and cultivation of salt tolerant varieties.【Method】A total of 102 Guangdong soybean landrace germplasm resources were selected as the experimental materials,and the germination potential,germination rate,salt injury index,hypocotyl length,radicle length, fresh weight and dry weight were measured after treatment with 150 mmol/L NaCl for salt stress,and distilled water was used as the control. The preliminary classification of salt tolerance was carried out by the salt injury index.The relative values of the remaining indexes were calculated and correlation analysis was performed. The principal component analysis method and membership function method were used to calculate the comprehensive evaluation D value,and the salt tolerance was ranked. Germplasms with strong salt tolerance were screened by cluster analysis method.【Result】According to the preliminary classification result of salt injury index,it was found that highly tolerant germplasm resources accounted for the largest proportion of the 102 germplasm resources,followed by tolerant germplasm resources,moderately tolerant and sensitive germplasm resources accounted for the same proportion,there was no sensitive germplasm resource. Compared with the control,the indexes of soybean germination stage were all reduced under salt stress,and their degree of inhibition was in the order of radicle length>hypocotyl length>fresh weight>dry weight>germination potential>germination rate,and radicle length at germination stage was more inhibited,while germination rate was less inhibited. The results of correlation analysis showed that there was a certain degree of correlation between most of the indexes. Through principal component analysis,two principal components were obtained with a cumulative contribution rate of 88.17%,in which the largest absolute value of the eigenvector in the first principal component(PC1) was relative germination potential, and the largest absolute value of the eigenvector in the second principal component(PC2) was relative germination rate and salt injury index. The two obtained principal components were used as two comprehensive indexes,and the salt tolerance was ranked according to D value. The numbers of top five germplasm resources were 374,310,358,357 and 309 respectively. The salt tolerant germplasm resources were selected by cluster analysis and the numbers were 310,358,374 and 331 respectively.【Conclusion】Four soybean landrace germplasm resources with strong salt tolerance are screened out,which can be used as parental materials for salt tolerant germplasm creation or salt resistant germplasms for development and utilization of salinized land. Relative germination potential and relative germination rate indexes can be used to evaluate salt tolerance of soybean and to screen salt tolerant germplasms.

     

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