Chilling requirement and related gene expression analysis during dormancy release of Queen Nina grape
-
摘要: 【目的】分析妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达，为该品种在广西葡萄产区引种推广及其休眠解除机制的研究提供理论参考。【方法】以3年生妮娜皇后葡萄为试验材料，采用0~7.2 ℃估算模型，对采后修剪枝段进行（4±1）℃下0、312、408和504 h共4个不同时长的低温处理，通过水培调查计算各低温时长处理枝段萌芽率。并对比3%浓度单氰胺破眠剂处理下的枝段萌芽率确定其需冷量范围；测定各处理枝段中可溶性总糖与淀粉含量；通过实时荧光定量PCR检测芽内5个芽休眠解除关键基因的相对表达量。【结果】妮娜皇后葡萄低温处理312 h后，水培4周萌芽率均高于50%，且均与相同低温处理取样后进行单氰胺处理的萌芽率无显著差异（P>0.05），结合采样前自然条件下0~7.2 ℃的低温积累时数37 h，将低温处理时间与自然积累的低温时数相加估算其需冷量<349 h；妮娜皇后葡萄芽休眠解除过程中，枝条中的淀粉含量显著降低（P<0.05，下同），淀粉转化为可溶性糖，可溶性糖含量显著增加。与未经低温处理（0 h）相比，低温处理后妮娜皇后芽中脱落酸（ABA）合成酶基因VvNCED1、ABA降解酶基因VvA8H4、ABA调节的胼胝质合成酶基因VvCALS1和胼胝质水解酶基因VvGLU1等4个关键休眠基因的相对表达量均显著增加，ABA合成酶基因VvXERICO相对表达量显著降低。【结论】低温处理能通过降低妮娜皇后葡萄枝条中淀粉含量，增加可溶性总糖含量，并进一步上调葡萄芽内ABA降解酶基因和胼胝质水解酶基因的表达解除芽休眠。妮娜皇后葡萄为低需冷量品种，适宜在广西葡萄产区种植。Abstract: 【Objective】The purpose of the study was to analyze the chilling requirement and related gene expression during dormancy release of Queen Nina grape,so as to provide a theoretical reference for the introduction and promotion of Queen Nina grape in Guangxi grape producing areas and the study of its dormancy release mechanism.【Method】Three-year-old Queen Nina grapes were used as experimental materials,and the 0-7.2℃ estimation model was used. The post-harvest pruning branches were treated at(4±1)℃ for four different chilling treatment times of 0,312,408 and 504 h. The germination rate of branches treated with different chilling treatment times was calculated by hydroponic investigation,and the range of chilling requirement was determined by comparing the germination rate of branches treated with 3% hydrogen cyanamide dormancy breaker. The contents of total soluble sugar and starch in branches of each treatment were determined. The relative expression of five key genes for dormancy release was determined by real-time fluorescence quantitative PCR.【Result】After 312 h of chilling treatment,the germination rate of Nina Queen grape was higher than 50% after 4 weeks of hydroponic culture,and there was no significant difference in the germination rate between all of them and that of the same chilling treatment sampled and then treated with hydrogen cyanamide(P>0.05). In addition, the chilling accumulation hours of 0~7.2℃ under natural conditions before sampling were 37 h. Therefore,by adding the chilling treatment time to the natural accumulation of chilling hours,the chilling requirement could be estimated to be<349 h. During the dormancy release process of Queen Nina grape buds,the starch content in the branches significantly decreased(P<0.05,the same below),and starch was converted into soluble sugars,with a significant increase in soluble sugar content. Compared with those without chilling treatment(0 h),the relative expression of the four key genes,including abscisic acid(ABA)synthase gene VvNCED1,ABA degrading enzyme gene VvA8H4,ABA regulated callose synthase gene VvCALS1 and callose hydrolase gene VvGLU1,significantly increased in Queen Nina buds after chilling treatment,while the relative expression of ABA synthase gene VvXERICO significantly decreased.【Conclusion】Chilling treatment can release bud dormancy by reducing starch content,increasing the total soluble sugar content in the branches of Queen Nina grape,and further up-regulating the expression of ABA degrading enzyme genes and callose hydrolase genes in the buds of the grape. Queen Nina grape is a low chilling requirement variety,which is suitable for planting in Guangxi grape producing area.
-
Keywords:
- Queen Nina grape /
- chilling requirement /
- dormancy release /
- gene expression /
- Guangxi
-
-
白耀栋,郝燕. 2020. 8个鲜食葡萄品种能用于河西设施栽培[J]. 甘肃林业科技,45(3):20-22.[Bai Y D,Hao Y. 2020.Introduction and screening of eight fresh grape variety in Hexi corridor area in Gansu Province[J]. Journal of Gansu Forestry Science and Technolgy,45(3):20-22.]doi:10. 3969/j.issn.1006-0960.2020.03.007. 郭春苗,杨波,木巴热克·阿尤普,许娟,车玉红,龚鹏. 2017.扁桃休眠期前后结果枝内碳水化合物代谢特征及其与坐果的关系[J]. 西北植物学报,37(11):2221-2229.[Guo C M,Yang B,Mubareke A,Xu J,Che Y H,Gong P. 2017.The metabolism of carbohydrate in the bearing branches and its relationship with fruit setting before and after period of dormancy in almond[J]. Acta Botanica BorealiOccidentalia Sinica,37(11):2221-2229.]doi: 10.7606/j.issn.1000-4025.2017.11.2221. 郭松涛. 2020. 宁夏设施葡萄需冷量与需热量及补光对葡萄萌芽影响研究[D]. 银川:宁夏大学.[Guo S T. 2020.Study on the chilling and heat requirements of grapes cultivars under protected culture and the effects of supplementary light on the germination of grapes in Ningxia[D]. Yinchuan:Ningxia University.]doi:10.27257/d.cnki.gnxhc. 2020.001089. 郝紫微,辛守鹏,李明,朱碧云. 2022. 7个鲜食葡萄品种在淮安地区的引种表现[J]. 北方果树,(4):14-15.[Hao Z W, Xin S P,Li M,Zhu B Y. 2022. Introduction performance of 7 table grape varieties in Huai'an[J]. Northern Fruits, (4):14-15.]doi: 10.16376/j.cnki.bfgs.2022.04.005. 李勃. 2011. 上海地区甜樱桃生物学特性及单氰胺打破休眠研究[D]. 上海:上海交通大学.[Li B. 2011. Studies on the biological characteristics of sweet cherry in Shanghai and the effect of hygrogen cyanamide on dormancy breaking[D]. Shanghai:Shanghai Jiao Tong University.] 秦洪波,郭伦发,江新能,韦婉羚,苏天明,谢代祖,李运飞,韦林,王新桂. 2020. 喀斯特地区不同核桃品种需冷量分析[J]. 南方农业学报,51(2):364-369.[Qin H B,Guo L F, Jiang X N,Wei W L,Su T M,Xie D Z,Li Y F,Wei L, Wang X G. 2020. Analysis of chilling requirement of different walnut cultivars in karst areas[J]. Journal of Southern Agriculture,51(2):364-369.]doi:10.3969/j.issn. 2095-1191.2020.02.016. 施招婉. 2019. 葡萄芽休眠解除过程中乙烯合成与信号途径关键基因的筛选与功能分析[D]. 广州:华南农业大学.[Shi Z W. 2019. Screening and functional analysis of key genes involved in ethylene biosynthesis and signaling pathways during grape bud dormancy release[D]. Guangzhou:South China Agricultural University.]doi: 10.27152/d.cnki.ghanu.2019.000945. 孙萍,沈建生,林贤锐,陈红星,陈辰斐,王轶. 2022. 浙中地区葡萄新品种(系)的引种表现和适应性研究[J]. 中国南方果树,51(2):139-142.[Sun P,Shen J S,Lin X R,Chen H X,Chen C F,Wang Y. 2022. Performance and adaptability of new grape cultivars(Lines)introduced in central Zhejiang[J]. South China Fruits,51(2):139-142.]doi:10. 13938/j.is sn.1007-1431.20210673. 王博,白扬,白先进,张瑛,谢太理,刘金标,陈爱军,娄兵海, 何建军,林玲,周咏梅,曹雄军. 2016. 阳光玫瑰葡萄在广西南宁的引种表现及其一年两收栽培技术[J]. 南方农业学报,47(6):975-979.[Wang B,Bai Y,Bai X J,Zhang Y, Xie T L,Liu J B,Chen A J,Lou B H,He J J,Lin L,Zhou Y M,Cao X J. 2016. Introduction performance and doubleharvest-a-year cultivation technique of'Shine Muscat'grape in Nanning,Guangxi[J]. Journal of Southern Agriculture,47(6):975-979.]doi:10.3969/j:issn.2095-1191. 2016.06.975. 王海波,韩晓,王孝娣,史祥宾,王宝亮,郑晓翠,王志强,冀晓昊,刘凤之. 2019. 不同砧木对87-1和'夏黑'设施葡萄品种需冷量的影响研究[J]. 中国果树,2019(5):46-49.[Wang H B,Han X,Wang X B,Shi X D,Wang B L, Zheng X C,Wang Z Q,Ji X H,Liu F Z. 2019. Effect of different rootstocks on chilling requirement of 87-1 and'Summer Black'grape cultivars in greenhouse[J]. China Fruits,2019(5):46-49.]doi: 10.16626/j.cnki.issn1000-8047.2019.05.010. 王海波,王孝娣,王宝亮,魏长存,刘万春,何锦兴,刘凤之. 2011. 设施葡萄常用品种的需冷量、需热量及2者关系研究[J]. 果树学报,28(1):37-41.[Wang H B,Wang X D, Wang B L,Wei C C,Liu W C,He J X,Liu F Z. 2011.Chilling and heat requirements and relationship between them for major grape cultivars under protected culture[J]. Journal of Fruit Science,28(1):37-41.]doi: 10.13925/j.cnki.gsxb.2011.01.004. 王西成,钱亚明,赵密珍,吴伟民,王庆莲. 2014. 设施葡萄萌芽调控中需冷量和需热量及其相互关系[J]. 植物生理学报,50(3):309-314.[Wang X C,Qian Y M,Zhao M Z, Wu W M,Wang Q L. 2014. Chilling and heat requirements and their relationship in budburst regulation of Vitis vinifera L. for protected cultivation[J]. Plant Physiology Journal,50(3):309-314.]doi: 10.13592/j.cnki.ppj.2014.03.014. 奚晓军,查倩,和雅妮,蒋爱丽,田益华. 2018. 上海地区'申沪'系列葡萄新品种的需冷量和需热量研究[J]. 中外葡萄与葡萄酒,(4):58-60.[Xi X J,Zha Q,He Y N,Jiang A L,Tian Y H. 2018. Chilling and heat requirements of'Shen/Hu'series grapevine cultivars in Shanghai region[J]. Sino-Overseas Grapevine & Wine,(4):58-60.]doi: 10.13414/j.cnki.zwpp.2018.04.013. 张鸿雁,陈莉,屈嘉燕,李少华,曾青青,张鹏,管雪强,张克坤,房玉林,陈可钦. 2022. 外源植物激素对'赤霞珠'葡萄冬芽萌发的影响[J]. 中外葡萄与葡萄酒,(5):52-58.[Zhang H Y,Chen L,Qu J Y,Li S H,Zeng Q Q,Zhang P, Guan X Q,Zhang K K,Fang Y L,Chen K Q. 2022. Effects of exogenous plant hormones on germination of grape winter buds[J]. Sino-Overseas Grapevine & Wine,(5):52-58.]doi: 10.13414/j.cnki.zwpp.2022.05.008. Hirose M,Kaku Y,Fujita Y,Watanabe H,Yasuno T,Ozekii Y, Nakao S. 2000. Chilling requirement and chemical treat-ment for breaking dormancy in grapevines,peaches and Japanese pears[J]. Bulletin of the Oita Prefectural Agricultural Research Center,30:1-13.
Ko J H,Yang S H,Han K H. 2006. Upregulation of an Arabidopsis RING-H2 gene,XERICO,confers drought tolerance through increased abscisic acid biosynthesis[J]. The Plant Journal,47(3):343-355. doi:10.1111/j.1365-313X.2006.0 2782.x.
Li J Z,Xu Y,Niu Q F,He L F,Teng Y W,Bai S L. 2018.Abscisic acid(ABA)promotes the induction and maintenance of pear(Pyrus pyrifolia white pear group)flower bud endodormancy[J]. International Journal of Molecular Sciences,19(1):310. doi: 10.3390/ijms19010310.
María A O A,Miguel A M T,Marisela R D,Miguel A H O, Irasema V Ao. 2020. Changes in the endogenous content and gene expression of salicylic acid correlate with grapevine bud dormancy release[J]. Journal of Plant Growth Regulation,40(1):254-262. doi:10.1007/s00344-020-10 100-9.
Mohamed H B,Vadel A M,Geuns J M C,Khemira H. 2010.Biochemical changes in dormant grapevine shoot tissues in response to chilling:Possible role in dormancy release[J]. Scientia Horticulturae,124(4):440-447. doi: 10.1016/j.scienta.2010.01.029.
Or E,Vilozny I,Fennell A,Eyal Y,Ogrodovitch A. 2002. Dormancy in grape buds:Isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release[J]. Plant Science, 162(1):121-130. doi: 10.1016/S0168-9452(01)00542-8.
Rinne P L H,Schoot C V D. 2003. Plasmodesmata at the crossroads between development,dormancy,and defense[J]. Canadian Journal of Botany,81(12):1182-1197. doi:10. 1139/b03-123.
Rinne P L H,Welling A,Vahala J,Ripel L,Ruonala R,Kangasjarvi J,Schoot C V D. 2011. Chilling of dormant buds hyperinduces FLOWERING LOCUS T and recruits GAinducible 1, 3-beta-glucanases to reopen signal conduits and release dormancy in Populus[J]. Plant Cell,23(1):130-146. doi: 10.1105/tpc.110.081307.
Singh R K,Maurya J P,Azeez A,Miskolczi P,Tylewicz S,Stojkovič K,Delhomme N,Busov V,Bhalerao R P. 2018. A genetic network mediating the control of bud break in hybrid aspen[J]. Nature Communications,9(1):1-10. doi: 10.1038/s41467-018-06696-y.
Singh R K,Miskolczi P,Maurya J P,Bhalerao R P. 2019. A tree ortholog of short vegetative phase floral repressor mediates photoperiodic control of bud dormancy[J]. Current Biology,29(1):128-133. doi:10.1016/j.cub.2018.11. 006.
Tylewicz S,Petterle A,Marttila S,Miskolczi P,Azeez A,Singh R K,Immanen J,Mahler N,Hvidsten T R,Eklund D M. 2018. Photoperiodic control of seasonal growth is mediated by ABA acting on cell-cell communication[J]. Science,360(6385):212-215. doi: 10.1126/science.aan8576.
Wu S W,Kumar R,Iswanto A B B,Kim J Y. 2018. Callose balancing at plasmodesmata[J]. Journal of Experimental Botany, 69(22):5325-5339. doi: 10.1093/jxb/ery317.
Zheng C L,Halaly T,Acheampong A K,Takebayashi Y,Jikumaru Y,Kamiya Y,Or E. 2015. Abscisic acid(ABA)regulates grape bud dormancy,and dormancy release stimuli may act through modification of ABA metabolism[J]. Journal of Experimental Botany,66(5):1527-1542. doi:10. 1039/jxb/eru519.
-
期刊类型引用(1)
1. 肖丽冰，钟挺威，李琳琳，陆秀边，林钦雄，韩佳宇，张延晖，贾海锋，白先进，王博. 不同葡萄砧木品种需冷量及低温时长对芽休眠相关基因表达的影响. 南方农业学报. 2024(08): 2237-2247 . 
本站查看
其他类型引用(1)
计量
- 文章访问数: 71
- HTML全文浏览量: 6
- PDF下载量: 9
- 被引次数: 2
下载: