盐胁迫对2个葡萄品种扦插苗光合特性及抗氧化能力的影响

Effects of salt stress on photosynthetic characteristics and antioxidant capacity of cutting seedlings of two grape varieties

  • 摘要: 【目的】研究盐胁迫对2个葡萄品种扦插苗光合特性及抗氧化能力的影响,为探究葡萄抗盐机理和盐碱土的改善及利用提供理论依据。【方法】设100 mmol/L NaCl浇灌巨峰和阳光玫瑰葡萄盆栽扦插苗为盐胁迫处理,清水灌溉为对照(CK),分别于胁迫0、7、14、21和28 d调查2个品种葡萄植株盐害症状并计算盐害指数;测定叶绿素荧光参数与过氧化物酶(POD)、超氧化物歧化酶(SOD)和抗坏血酸过氧化氢酶(APX)活性及丙二醛(MDA)、脯氨酸(Pro)和可溶性蛋白含量;对2个葡萄品种在盐害胁迫处理下的各生长与生理指标进行主成分分析和隶属函数分析。【结果】巨峰和阳光玫瑰2个葡萄品种的盐害症状均表现为盐胁迫前期老叶首先出现叶缘焦枯并伴有褐色斑点,胁迫后期叶片失绿萎蔫至枯黄。阳光玫瑰表现盐害症状较巨峰早;2个葡萄品种的株高、茎粗和叶片数随盐害胁迫天数的增加均呈先升高后降低的变化趋势;胁迫处理28 d时,阳光玫瑰盐害指数(87.29%)明显高于巨峰盐害指数(31.67%)。2个葡萄品种的叶片叶绿素含量、PSⅡ最大光化学产量(Fv/Fm)、实际光化学效率Y(Ⅱ)和光化学淬灭系数(qP)总体上呈下降趋势;非光化学淬灭系数(NPQ)、POD和SOD活性呈先升高后降低的变化趋势,而非调节能量耗散的量子产额Y(NO)、APX活性和MDA、Pro和可溶性蛋白含量均呈上升趋势;巨峰和阳光玫瑰在盐害胁迫下的平均隶属函数值为0.52和0.51,巨峰的盐胁迫耐受性略高于阳光玫瑰。【结论】盐害胁迫下,巨峰和阳光玫瑰2个葡萄品种对盐害胁迫的响应趋势相同但响应程度存在差异,巨峰的盐耐受性高于阳光玫瑰;叶绿素含量、Fv/Fm、NPQ、qP、Y(Ⅱ)、Y(NO)、POD活性和Pro含量可作为葡萄耐盐性的评价标准。

     

    Abstract: 【Objective】To study the effect of salt stress on the photosynthetic characteristics and antioxidant capacity of cutting seedlings of two grape varieties,in order to provide theoretical basis for investigating the mechanism of salt resistance of grapes and the improvement and utilization of saline soils.【Method】Set 100 mmol/L NaCl irrigation of Kyoho and Shine Muscat grape pot cuttings as salt stress treatment and water irrigation as control (CK),and investigated the salt damage symptoms of the two varieties of grape plants at 0,7,14,21 and 28 d of stress respectively,calculated salt damage index;and measured the photosynthetic indexes and the activities of peroxidase (POD),superoxide dismutase(SOD),and ascorbic acid catalase (APX),as well as malondialdehyde (MDA),proline (Pro) and soluble protein contents;and the growth and physiological indexes of the two grape varieties under salt stress treatment were analyzed by principal component analysis and affiliation function analysis.【Result】The salt damage symptoms of both Kyoho and Shine Muscat varieties showed that the old leaves first appeared scorched with brown spots at the beginning of the salt stress period,and the leaves of the plants lost their green color and wilted to yellow at the end of the stress period.Shine Muscat showed salt stress symptoms earlier than Kyoho;the plant height,stem diameter and number of leaves of the two grape varieties showed a trend of increasing and then decreasing with the increase in the number of days of salt stress;after 28 d of stress treatment,the salt damage index of Shine Muscat (87.29%) was higher than that of Kyoho (31.67%).The chlorophyll content of the leaves of two grape varieties,the maximum photochemical yield of PSⅡ(Fv/Fm) the actual photochemical efficiency Y(PSⅡ)and the coefficient of photochemical quenching (qP) showed an overall decreasing trend;and the value of the non-photochemical quenching coefficient (NPQ),POD and SOD activities showed an increasing and then decreasing trend,while quantum yield of non-regulated energy dissipationY (NO),APX activity and MDA,Pro and soluble protein contents showed an increasing trend;and the average affiliation function values for Kyoho and Shine Muscat under salt stress were 0.52 and 0.51 respectively,and the salt stress tolerance of Kyoho was slightly higher than that of Shine Muscat.【Conclusion】Under salt stress,the two grape varieties,Kyoho and Shine Muscat,show the same trend of response to salt stress but with differences in the degree of response,and Kyoho is more tolerant to salt than Shine Muscat;the chlorophyll content,Fv/Fm,NPQ,qP,Y(Ⅱ),Y(NO),POD activity and Pro content can be used as evaluation criteria for salt tolerance in grapes.

     

/

返回文章
返回