Abstract: 【Objective】 Cloning and identifying cassava root tissue-specific promoters to provide reference for further analysis and identification of the functions of key genes in the development of cassava storage root and the regulation mechanism of starch synthesis.【Method】 The specific high expression genes were identified in cassava storage root and primary root on the basis of transcriptomic data of different tissues in cassava and the results of real-time fluorescence quantitative PCR detection. PLA website was used to analyze the 1464 bp promoter sequence in the upstream region of the genes. According to the distribution of the root-specific cis acting element ROOTMOTIFTAPOX1(RMT1),the sequence was truncated for analysis, and its specific primers were designed, promoter sequences with the length of 1464,705 and 319 bp were obtained through PCR amplification. The promoters were fused with the β-glucosidase(GUS) gene and transferred into Arabidopsis thaliana by the mediation of Agrobacterium tumefaciens. GUS staining and GUS activity determination were performed in transgenic plants to identify tissue specificity and promoter activity of the promoters. 【Result】 Based on transcriptomic data from different tissues in cassava, three candidate genes which specifically highly expressed in primary root,storage root and root apical meristem were selected:MeHPS(Phytozome13 ID:Manes. 01G078200),MeSR2(Phytozome13 ID:Manes.04G017600) and MeSR3(Phytozome13 ID:Manes.14G006300). The specific highe xpression gene MeHPS in cassava storage root and primary root was identified by the results of real-time fluorescence quantitative PCR detection. The analysis of MeHPS gene promoter suggested that the 1464 bp promoter sequence contained five cisacting elements RMT1. The GUS staining results of transgenic plants showed that p1464 promoter had obvious root specificity,p705 promoter had conducting tissue and root specificity while p319 promoter basically lost tissue specificity. Determination results of GUS activity in roots demonstrated that the GUS activities driven by p1464, p705 and p319 were 8.33, 7.87 and 10.52 U/g respectively, which were all significantly higher than that driven by 35S promoter(6.69 U/g) (P<0.05).【Conclusion】 MeHPS gene is specifically and highly expressed in roots,and its p1464 promoter has root-specific expression activity, which can be used to specifically drive high-level transcription of target genes in roots. p705 promoter has strong conducting tissue specificity and can be used to drive the transcription of protein genes with transporting function. And p319 promoter,regarded as constitutive promoter,can partially replace 35S promoter.