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阿维菌素对瓜实蝇3种解毒酶系的影响

姜建军 王凤英 陈黔 黄立飞 曹雪梅 杨朗

姜建军, 王凤英, 陈黔, 黄立飞, 曹雪梅, 杨朗. 阿维菌素对瓜实蝇3种解毒酶系的影响[J]. 南方农业学报, 2023, 54(7): 1893-1902. doi: 10.3969/j.issn.2095-1191.2023.07.001
引用本文: 姜建军, 王凤英, 陈黔, 黄立飞, 曹雪梅, 杨朗. 阿维菌素对瓜实蝇3种解毒酶系的影响[J]. 南方农业学报, 2023, 54(7): 1893-1902. doi: 10.3969/j.issn.2095-1191.2023.07.001
JIANG Jian-jun, WANG Feng-ying, CHEN Qian, HUANG Li-fei, CAO Xue-mei, YANG Lang. Effects of abamectin on the three detoxification enzyme systems in Bactrocera cucurbitae(Coquillett)[J]. Journal of Southern Agriculture, 2023, 54(7): 1893-1902. doi: 10.3969/j.issn.2095-1191.2023.07.001
Citation: JIANG Jian-jun, WANG Feng-ying, CHEN Qian, HUANG Li-fei, CAO Xue-mei, YANG Lang. Effects of abamectin on the three detoxification enzyme systems in Bactrocera cucurbitae(Coquillett)[J]. Journal of Southern Agriculture, 2023, 54(7): 1893-1902. doi: 10.3969/j.issn.2095-1191.2023.07.001

阿维菌素对瓜实蝇3种解毒酶系的影响

doi: 10.3969/j.issn.2095-1191.2023.07.001
基金项目: 

国家自然科学地区基金项目(31960564);广西科技重大专项(桂科AA20108002);广西农业科学院科技发展基金项目(桂农科2021JM72,桂农科2021YT068)

详细信息
    作者简介:

    姜建军(1980-),https://orcid.org/0000-0003-1836-6900,博士,副研究员,主要从事果蔬害虫抗药性监测与抗性机制研究工作,E-mail:jiangjianjun8008@126.com

    通讯作者:

    杨朗(1976-),https://orcid.org/0009-0009-7274-9663,博士,研究员,主要从事农业昆虫与害虫防治研究工作,E-mail:107561466@qq.com

  • 中图分类号: S433

Effects of abamectin on the three detoxification enzyme systems in Bactrocera cucurbitae(Coquillett)

Funds: 

Regional Project of National Natural Science Foundation of China(31960564);Guangxi Science and Technology Key Project(Guike AA20108002);Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences (Guinongke 2021JM72,Guinongke 2021YT068)

  • 摘要: 【目的】探讨阿维菌素对瓜实蝇[Bactrocera cucurbitae(Coquillett)]解毒酶系的影响,明确瓜实蝇阿维菌素抗性与解毒酶的关系,为瓜实蝇抗药性综合治理和科学用药提供理论基础。【方法】采用饲毒法测定阿维菌素对瓜实蝇成虫敏感品系(SS)和抗阿维菌素品系(RS)的亚致死浓度(LC25)及致死中浓度(LC50);利用微孔板法测定SS品系和RS品系经阿维菌素LC25、LC50剂量处理12、24、48和72 h后其体内谷胱甘肽S-转移酶(Glutathione S-transferase,GSTs)、羧酸酯酶(Carboxylesterase,CarE)活性,采用ELISA试剂盒法测定细胞色素P450单加氧化酶(CytochromeP450 monooxygenases,P450s)含量;测定比较SS品系和RS品系GSTs、CarE米氏常数(Km)及最大反应速率(Vmax)的变化及增效剂顺丁烯二酸二乙酯(DEM)、磷酸三苯酯(TPP)和增效醚(PBO)对阿维菌素的增效作用。【结果】与SS品系相比,RS品系体内GSTs和CarE活性均显著上升(P<0.05,下同),分别为SS品系的2.92和1.46倍,P450s含量则显著下降,为SS品系的68.0%。SS品系在LC25剂量的阿维菌素处理后12、48 h及LC50处理后12、24、48 h和RS品系在LC25剂量处理后12 h、LC50处理后72 h,其体内GSTs活性均被诱导显著增强; LC25剂量处理后24 h,SS品系体内CarE活性被诱导显著增强,RS品系中LC25处理后72 h和LC50处理后各时间段CarE活性表现为被显著抑制; LC50处理后12 h,P450s含量在SS品系中表现为显著降低,而在72 h则显著升高。与SS品系相比,RS品系GSTs的Km显著降低,Vmax显著升高,CarE的KmVmax变化不明显;增效剂DEM、TPP和PBO在SS品系中的增效比分别为1.03、1.04和2.08,在RS品系中的增效比分别为3.45、2.52和2.26,相对增效系数分别为0.71、0.60和0.54。【结论】 GSTs和CarE解毒酶参与了瓜实蝇对阿维菌素的抗性形成,其中GSTs活性发生了量变和质变,其对底物亲和力增加和代谢速度增快,在瓜实蝇对阿维菌素抗性形成中起到主导作用。
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  • 收稿日期:  2023-02-07

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