Abstract: 【Objective】To explore the adaptability of Panax notoginseng（Burk.）F. H. Chen seedlings which introduced from different seedling nursery sites to different under-forest planting sites of pine tree，then provide theoretical basis for planting P. notoginseng in pine forest in Yunnan.【Method】 A cross-introduction experiment was set up that P. notoginseng seedlings bred in low-latitude（hereinafter referred to as LL）areas and high-latitude（hereinafter referred to as HL）areas of Yunnan were cross-introduced to the LL pine forest planting site and HL pine forest planting site in Yunnan respectively. The emergence rate，survival rate and incidence rate of P. notoginseng after introduction were investigated in the field. The biomass of P. notoginseng plants was measured，and the drying rate and root-shoot ratio were calculated. Finally，the total saponin content of P. notoginseng after introduced to under-forest sites of pine trees were determined by liquid chromatography，to evaluate the adaptability of seedlings introduced from different seedling sources to cultivation under pine forest.【Result】 In the middle of April and the end of April，the emergence rate of LL seedlings reached 65.00% and 87.67%，which were significantly higher than that of HL seedlings（53.05% and 70.00%） （P＜0.05， the same below） at LL planting site. At the end of March，the seedling emergence rate of HL seedlings was 31.05%，which was significantly higher than that of LL seedlings（11.30%）at HL planting site. Furthermore，at LL planting site， the incidence of root rot and black spot diseases of LL seedlings was lower than that of HL seedlings，but there were no significant differences in HL planting sites between two source seedlings（P＞0.05，the same below）. Besides，the biomass，drying rate，and rootshoot ratio of LL seedlings were better than that of HL at LL planting site. At HL planting sites，the biomass，drying rate and root-shoot ratio of LL seedlings had no significant difference from those of HL seedlings. Finally，the saponin content of LL seedlings was significantly higher than that of HL seedlings at LL planting site，but there were no significant difference in HL planting sites between two seedlings.【Conclusion】Introducing seedlings nearby is more conducive to the emergence，growth，and accumulation of saponin content of P. notoginseng. When planting P. notoginseng in pine forest， priority should be given to selecting seedlings with small difference in longitude and latitude and similar environmental conditions for transplanting.