Abstract: 【Objective】 This study clarified the taxonomic status and biological characteristics of the pathogen of honeysuckle powdery mildew in Guizhou and screened effective control agents，so as to provide a theoretical basis for disease control in the field.【Method】In this study，typical diseased leaf samples and flower buds of honeysuckle powdery mildew in Yanhe County， Tongren City and Suiyang County，Zunyi City，Guizhou Province were collected. Pathogenicity of collected strains was determined. The pathogen was identified by morphological characteristics，ITS sequence analysis， and phylogenetic tree analysis. The effects of temperature on the germination rate and pathogenicity of conidia of the pathogen were analyzed by the spore germination method and the spray inoculation method. The prevention effect of six common fungicides on honeysuckle powdery mildew was evaluated under indoor and greenhouse conditions respectively. 【Result】 Thirty-three suspected disease strains were obtained from planting bases of honeysuckle in Zunyi City and Tongren City，Guizhou Province. Pathogenicity test results showed that all strains were the pathogenic strain of honeysuckle powdery mildew. The morphological characteristics of the representative strain BF1 were similar to those of Erysiphe lonicerae var. lonicerae，and its ITS sequence clustering analysis showed that the pathogen was clustered with E. lonicerae var. lonicerae in the same branch of the phylogenetic tree.According to strain BF1 biological characteristics test， the optimum spore germination temperature of strain BF1 was 24-26 ℃；the optimum pathogenic temperature was 24 ℃. According to the results of screening control agents，under indoor conditions，1% polyoxin，30% pyraclostrobin，5% hexaconazole， and 0.3% tetramycin showed good inhibitory effects on conidial germination of strain BF1，with EC₅₀ values of 0.3454， 0.4347，0.5369，and 0.6315 g/L respectively；under greenhouse conditions，1% polyoxin，30% pyraclostrobin，5% hexaconazole，and 0.3% tetramycin showed good control effects on honeysuckle powdery mildew，with efficacies of 88.53%， 86.40%，84.00%，and 89.60% after twice treatments respectively.【Conclusion】The causative pathogen of honeysuckle powdery mildew in Tongren City and Zunyi City，Guizhou Province is E. lonicerae var. lonicerae. 0.3% tetramycin aquae， 1% polyoxin aquae，30% pyraclostrobin suspending agent and 5% hexaconazole emulsion can effectively prevent and control the disease，which can be applied in the production of honeysuckle.