Transcriptomic analysis of ovary of Procambarus clarkii under sun-drying environmental stress
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摘要: 【目的】运用转录组测序技术探究不同晒田环境胁迫下克氏原螯虾卵巢的基因表达变化，筛选出与其卵巢发育相关的候选基因及信号通路，为研究晒田胁迫方式促进克氏原螯虾卵巢发育的作用机制提供理论依据。【方法】以雌性克氏原螯虾为研究对象，分别在晒田环境胁迫0 d （CK）、3 d （T1）、7 d （T2）和10 d （T3）时采样，构建卵巢cDNA文库进行转录组测序，对差异表达基因（DEGs）进行GO功能注释及KEGG信号通路富集分析。【结果】共获得86377个Unigenes，在Nr、KOG/COG、Swiss-Prot和KEGG库中成功注释到22712个Unigenes；获得1115个胁迫组（T1、T2和T3）与CK的DEGs。KEGG信号通路富集分析结果表明，DEGs富集的PI3K-Akt信号通路、GnRH信号通路和雌激素信号通路与卵巢发育相关。实时荧光定量PCR验证结果表明，3个DEGs的表达模式与转录组分析结果基本一致，证明转录组数据准确可靠。筛选得到B2M、TUBA1B、MT-CYB、MT-ND1、MT-ND2、MT-ND4、DSX、Pck2、PIM3和serine protease nudel-like等10个与卵巢发育相关的DEGs，并对其表达量进行分析，结果表明各基因在晒田胁迫期间均可调控卵巢发育。【结论】 B2M、TUBA1B、MT-CYB、MT-ND1、MT-ND2、MT-ND4、DSX、Pck2、PIM3和serine protease nudel-like等10个DEGs及PI3K-Akt信号通路、GnRH信号通路、雌激素信号通路等3个富集通路在晒田胁迫期间参与卵巢发育调控。Abstract: 【Objective】The purpose of the study was to explore the gene expression changesin ovaries of Procambarus clarkii under different sun-drying stresses by transcriptomic sequencing technology, and to screen outthe candidate genes and signaling pathways related to ovarian development,so as toprovide a theoretical basis for studying the mechanism of sun-drying stress in promoting the ovarian development of P. clarkii.【Method】The female P. clarkii were sampled at 0 d(CK),3 d(T1),7 d(T2)and 10 d(T3)of sun-drying environmental stress,and ovary cDNA libraries were constructed for transcriptome sequencing,and the differentially expressed genes(DEGs)were subjected to GO functional annotation and KEGG signaling pathway enrichment analysis.【Result】A total of 86377 Unigenes were obtained. Of these, 22712 Unigenes were successfully annotated in Nr,KOG/COG, Swiss-Prot and KEGG databases. A total of 1115 DEGs were obtained between the stress group(T1,T2,T3)and the CK. The results of KEGG signaling pathway enrichment analysis showed that DEGs enriched on the pathways associated with ovarian development, including PI3K-Akt signaling pathway,GnRH signaling pathway and estrogen signaling pathway. The real-time fluorescence quantitative PCR verification results showed that the expression patterns of the three DEGs was basically consistent with the results of transcriptome analysis,proving that the transcriptome data were accurate and reliable. The ten DEGs related to ovarian development were screened out, including B2M, TUBA1B, MT-CYB, MT-ND1, MT-ND2, MT-ND4, DSX, Pck2, PIM3, and serine protease nudel-like. The expressions of these DEGs were analyzed, and the results showed that these genes regulated ovarian development during the period of sun-drying environmental stress.【Conclusion】 In this study,ten DEGs including B2M, TUBA1B,MT-CYB,MT-ND1,MT-ND2,MT-ND4, DSX,Pck2,PIM3 and serine protease nudel-like, and three enriched pathway sincluding PI3K-Akt signaling pathway, GnRH signaling pathway, and estrogen signaling pathway are involved in the regulation of ovarian development during the period of sun-drying environmental stress.
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刘国锋, 徐增洪, 朱光艳, 水燕, 何俊, 张宪中. 2021. 温室大棚高效繁育克氏原螯虾种苗技术[J]. 江苏农业科学, 49(17):160-165.[Liu G F, Xu Z H, Zhu G Y, Shui Y, He J, Zhang X Z. 2021. High-efficiency breeding technology of Procambarus clarkia shrimps in greenhouse[J]. Jiangsu Agricultural Science, 49(17):160-165.]doi: 10.15889/j.issn.1002-1302.2021.17.029. 刘汉杰. 2018. 自噬在N-乙酰半胱氨酸萝卜硫素抗胶质母细胞瘤中的作用及其机制研究[D]. 石家庄:河北医科大学.[Liu H J. 2018. Study on the effect and mechanism of autophagy in Sulforaphane-N-acetyl-cysteine anti-human glioblastomacells[D]. Shijiazhuang:Hebei Medical University.]doi: 10.7666/d.D01513692. 秦敏, 赖伟华, 李汉平, 钟诗龙. 2020. mtDNA变异对中国冠心病患者血脂水平的影响[J]. 实用药物与临床, 23(5):396-403.[Qin M, Lai W H, Li H P, Zhong S L. 2020.Effect of mitochondrial DNA mutations on plasma lipid concentration in Chinese patients with coronary artery disease[J] Practical Medicine and Clinic, 23(5):396-403.]doi:10.14053/j.cnki.ppcr. 202005003. 宋光同, 丁凤琴, 武松, 陈静, 汪翔, 侯冠军. 2015. 克氏原螯虾人工诱导工厂化繁育关键技术研究[J]. 水产科技情报, 42(2):108-112.[Song G T, Ding F Q, Wu S, Chen J,Wang X, Hou G J. 2015. Study on key technologies of artificial induction and industrialized breeding of Procambarus clarkii[J]. Aquatic Science and Technology Information, 42(2):108-112.]doi:10.16446/j.cnki.1001-1994. 2015.02.012. 宋光同, 江河, 王芬, 蒋业林, 陈祝, 王佳佳, 吴仓仓. 2022. 温棚保温、亲本规格对克氏原螯虾苗繁育效果的影响[J].水产科学, 41 (1):130-136.[Song G T, Jiang H, Wang F,Jiang Y L, Chen Z, Wang J J, Wu C C. 2022. Effect of greenhouse and broodstock size on breeding performance of red swamp crayfish Procambarus clarkii[J]. Fisheries Science, 41(1):130-136.]doi:10.16378/j.cnki. 1003-1111.20087. 孙珂. 2019. 克氏原螯虾室内养殖与繁育生态学研究[D]. 荆州:长江大学.[Sun K. 2019. Ecology study on indoor culture and breeding of Procambarus clarkii[D]. Jingzhou:Changjiang University.] 王庆, 杨家新, 匡腾蛟, 朱欢喜, 蒋琦辰, 周国勤, 周国平, 朱银安. 2012. 冬季温度刺激对克氏原螯虾繁育的影响[J].淡水渔业, 42 (2):93-96.[Wang Q, Yang J X, Kuang T J,Zhu H X, Jiang Q C, Zhou G Q, Zhou G P, Zhu Y A. 2012. Effect of warming up to stimulate Procambarus clarkii to breed in winter[J]. Freshwater Fishries, 42(2):93-96.]doi: 10.3969/j.issn.1000-6907.2012.02.017. 王庆. 2012. 克氏原螯虾繁育机制及养殖生态学研究[D]. 南京:南京师范大学.[Wang Q. 2012. Research on the breeding mechanism and aquaculture ecology of Procambarus clarkii[D]. Nanjing:Nanjing Normal University.]doi: 10.7666/d.Y2176313. 文玲梅, 赵伟, 金芳华, 张阳. 2018. 克氏原螯虾温室大棚苗种繁育技术[J]. 湖北农业科学, 57(S2):168-169.[Wen L M, Zhao W, Jin F H, Zhang Y. 2018. The larval breeding technique of Procambarus clarkia in greenhouse[J]. Hubei Agricultural Science, 57 (S2):168-169.]doi: 10.14088/j.cnki.issn0439-8114.2018.S2.053. 奚业文, 徐忠有, 胡宝, 胡桂友, 王顺昌. 2021. 克氏原螯虾稻田生态繁育试验分析[J]. 科学养鱼,(9):12-14.[Xi Y W, Xu Z Y, Hu B, Hu G Y, Wang S C. 2021. Analysis on ecological breeding experiment of Procambarus clarkii in paddy field[J]. Scientific Fish Farming,(9):12-14.]doi: 10.14184/j.cnki.issn1004-843x.2021.09.007. 徐增洪, 周鑫, 水燕, 赵朝阳. 2014. 克氏原螯虾繁殖行为生态学的实验研究[J]. 中国水产科学, 21(2):382-389.[Xu Z H, Zhou X, Shui Y, Zhao C Y. 2014. Experiment study on reproductive behavioral ecology of crayfish Procambarus clarkii[J]. China Fisheries Science, 21(2):382-389.]doi: 10.3724/SP.J.1118.2014.00382. 许洪杰, 陈浩, 王晓鹏, 徐增洪, 邢华, 陈健翔. 2020. 克氏原螯虾工厂化离体育苗技术研究[J]. 科学养鱼,(9):11-13.[Xu H J, Chen H, Wang X P, Xu Z H, Xing H, Chen J X. 2020. Study on the technology of industrialized isolated sports seedlings of Procambarus clarkii[J]. Scientific Fish Culture,(9):11-13.]doi:10.14184/j.cnki.issn1004-843x. 2020.09.007. 张聚涛. 2011. 克氏原螯虾工厂化繁育关键技术研究[D]. 扬州:扬州大学.[Zhang J T. 2011. Study on key techniques of the industrialized reproduction and its culture for Procambarus clarkii[D]. Yangzhou:Yangzhou University.]doi: 10.7666/d.y2049783. 周伟. 2014. 蚤状溞生殖转换相关功能基因doublesex1的研究[D]. 宁波:宁波大学.[Zhou W. 2014. Cloning and expression analysis of doublesex1 gene correlated switch from different reproduction modes of Daphnia pulex[D].Ningbo:Ningbo University.]doi: 10.7666/d.D604456. Amano M, Amiya N, Okumura T, Kado R. 2021. Immunohistochemical localization of a GnRH-like peptide in the nerve ganglion of three classes of crustaceans, the tadpole shrimp Triops longicaudatus (Branchiopoda), the Barnacle Balanus crenatus(Hexanauplia),and the hermit crab Pagurus filholi(Malacostraca)[J]. Zoological Science, 38 (1):51-59. doi: 10.2108/zs200097.
An Z H, Zhang Y Y, Sun L S. 2018. Effects of dietary astaxanthin supplementation on energy budget and bioaccumulation in Procambarus clarkii(Girard, 1852)crayfish under microcystin-LR stress[J]. Toxins, 10(7):277. doi:10. 3390/toxins10070277.
Arambourou H, Llorente L, Moreno-Ocio I, Herrero Ó, Barata C, Fuertes I, Delorme N, Méndez-Fernández L, Planelló R. 2020. Exposure to heavy metal-contaminated sediments disrupts gene expression, lipid profile, and life history traits in the midge Chironomus riparius[J]. Water Research, 168:115165. doi: 10.1016/j.watres.2019.115165.
Asati V, Mahapatra D K, Bharti S K. 2019. PIM kinase inhibitors:Structural and pharmacological perspectives[J]. European Journal of Medicinal Chemistry, 172:95-108. doi: 10.1016/j.ejmech.2019.03.050.
Bliss S P, Navratil A M, Xie J, Roberson M S. 2010. GnRH signaling, the gonadotrope and endocrine control of fertility[J]. Frontiers in Neuroendocrinology, 31(3):322-340.doi: 10.1016/j.yfrne.2010.04.002.
Cadenas J, Pors S E, Nikiforov D, Zheng M G, Subiran C,Bøtkjær J A, Mamsen L S, Kristensen S G, Andersen C Y. 2022. Validating reference gene expression stability in human ovarian follicles, oocytes, cumulus cells, ovarian medulla, and ovarian cortex tissue[J]. International Journal of Molecular Sciences, 14:23(2):886. doi: 10.3390/ijms23020886.
Capanni F, Greco S, Tomasi N, Giulianini P G, Manfrin C. 2021. Orally administered nano-polystyrene caused vitellogenin alteration and oxidative stress in the red swamp crayfish(Procambarus clarkii)[J]. Science of the Total Environment, 791:147984. doi:10.1016/j.scitotenv.2021. 147984.
Chehin M B, Fraietta R, Lorenzon A R, Bonetti T C S, Motta E L A. 2020. The insulin signaling pathway is dysregulated in cumulus cells from obese, infertile women with polycystic ovarian syndrome with an absence of clinical insulin resistance[J]. Therapeutic Advances in Reproductive Health, 14:2633494120906866. doi:10.1177/2633494120 906866.
Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp:An ultra-fast allin-one FASTQ preprocessor[J]. Bioinformatics, 34(17):i884-i890. doi: 10.1093/bioinformatics/bty560.
Chen Y T. 2015. The role of toll signaling in dorsoventral axis formation in the milkweed bug Oncopeltus fasciatus[D]. Albertus Magnus Platz:Universität zu Köln.
Childs G V, Odle A K, MacNicol M C, MacNicol A M. 2021.The importance of leptin to reproduction[J]. Endocrinology, 162 (2):bqaa204. doi:10.1210/endocr/bqaa204.Choi J H, Lee K T, Leung P C. 2011. Estrogen receptor alpha pathway is involved in leptin-induced ovarian cancer cell growth[J]. Carcinogenesis, 32(4):589-596. doi:10.1093/carcin/bgq276.
Conesa A, Götz S, García-Gómez J M, Terol J, Talón M, Robles M. 2005. Blast2GO:A universal tool for annotation,visualization and analysis in functional genomics research[J]. Bioinformatics, 21 (18):3674-3676. doi: 10.1093/bioinformatics/bti610.
Costa C, Semedo M, Machado S P, Cunha V, Ferreira M, Urbatzka R. 2021. Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 250(2):109170. doi: 10.1016/j.cbpc.2021.109170.
Cui J, Shen Y, Li R N. 2013. Estrogen synthesis and signaling pathways during aging:From periphery to brain[J]. Trends in Molecular Medicine, 19(3):197-209. doi: 10.1016/j.molmed.2012.12.007.
Diaz-Sotomayor M, Quezada-Calvillo R, Avery S E, Chacko S K, Yan L K, Lin A H M, Ao Z H, Hamaker B R, Nichols B L. 2013. Maltase-glucoamylase modulates gluconeogenesis and sucrase-isomaltase dominates starch digestion glucogenesis[J]. Journal of Pediatric Gastroenterology Nutrition, 57 (6):704-712. doi:10.1097/MPG.0b013e3182a 27438.
Ding G Y, Zheng C W, Wang B, Zhang L F, Deng D, Li Q,Guo H Z, Zhang S H, Xu Q Q. 2022. Transcriptome sequencing reveals the antiviral innate immunity by IFN-γ in Chinese sturgeon macrophages[J]. Frontiers in Immunology, 13:854689. doi: 10.3389/fimmu.2022.854689.
Dong Y N, Lyu L K, Zhang D Q, Li J, Wen H S, Shi B. 2021.Integrated lncRNA and mRNA transcriptome analyses in the ovary of cynoglossus semilaevis reveal genes and pathways potentially involved in reproduction[J]. Frontiers in Genetics, 12:671729. doi:10.3389/fgene. 2021. 671729.
Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q,Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, Di P F, Birren B W, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 29 (7):644-652. doi: 10.1038/nbt.1883.
Guo Y S, Jiang W, Yu W L, Niu X, Liu F J, Zhou T, Zhang H, Li Y, Zhu H, Zhou Z M, Sha J H, Guo X J, Chen D Z. 2019. Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility[J]. Journal of Proteomics, 208:103478. doi: 10.1016/j.jprot.2019.103478.
He Y D, Li X W, Jia D T, Zhang W M, Zhang T, Yu Y, Xu Y H, Zhang Y J. 2019. A transcriptomics-based analysis of the toxicity mechanisms of gabapentin to zebrafish embryos at realistic environmental concentrations[J]. Environmental Pollution, 251:746-755. doi:10.1016/j.envpol. 2019.05.063.
Hernández-Aguirre L E, Cota-Ruiz K, Peregrino-Uriarte A B,Gómez-Jiménez S, Yepiz-Plascencia G. 2021. The gluconeogenic glucose-6-phosphatase gene is expressed during oxygen-limited conditions in the white shrimp Penaeus(Litopenaeus)vannamei:Molecular cloning,membrane protein modeling and transcript modulation in gills and hepatopancreas[J]. Journal of Bioenergetics and Biomembranes, 53 (4):449-461. doi: 10.1007/s10863-021-09903-6.
Jiang H C, Xing Z J, Lu W, Qian Z J, Yu H W, Li J L. 2014.Transcriptome analysis of red swamp crawfish Procambarus clarkii reveals genes involved in gonadal development[J]. PLoS One, 9(8):e105122. doi: 10.1371/journal.pone.0105122.
Jiang X R, Yao W X, Yang H, Tan S M, Leng X J, Li X Q. 2021. Dietary effects of Clostridium autoethanogenum protein substituting fish meal on growth, intestinal histology and immunity of Pacific white shrimp(Litopenaeus vannamei) based on transcriptome analysis[J]. Fish & Shellfish Immunology, 119:635-644. doi:10.1016/j.fsi. 2021.10.005.
Jiao T, Yang T T, Wang D, Gao Z Q, Wang J L, Tang B P,Liu Q N, Zhang D Z, Dai L S. 2019. Characterization and expression analysis of immune-related genes in the red swamp crayfish, Procambarus clarkii in response to lipopolysaccharide challenge[J]. Fish & Shellfish Immunology, 95:140-150. doi: 10.1016/j.fsi.2019.09.072.
Laufer H, Biggers W J, Ahl J S. 1998. Stimulation of ovarian maturation in the crayfish Procambarus clarkii by methyl farnesoate[J]. General and Comparative Endocrinology, 111 (2):113-118. doi: 10.1006/gcen.1998.7109.
Lee Y, Kim N, Roh H, Kim A, Han H J, Cho M, Kim D H. 2021. Transcriptome analysis unveils survival strategies of streptococcus parauberis against fish serum[J]. PLoS One, 16 (5):e0252200. doi: 10.1371/journal.pone.0252200.
LeMosy E K, Kemler D, Hashimoto C. 1998. Role of Nudel protease activation in triggering dorsoventral polarization of the Drosophila embryo[J]. Development, 125(20):4045-53. doi: 10.1242/dev.125.20.4045.
Li B, Dewey C N. 2011. RSEM:Accurate transcript quantification from RNA-Seq data with or without a reference genome[J]. BMC Bioinformatics, 12 (1):323. doi: 10.1186/1471-2105-12-323.
Li J Z, Bai L. 2020. The role of PDE5a in oocyte maturation of zebrafish[J]. General and Comparative Endocrinology, 286:113303. doi: 10.1016/j.ygcen.2019.113303.
Li L, Dong M, Wang X G. 2016. The implication and significance of Beta 2 microglobulin:A conservative multifunctional regulator[J]. Chinese Medical Journal, 129(4):448-455. doi: 10.4103/0366-6999.176084.
Li Z F, He X Y, Zhang X S, Zhang J L, Guo X F, Sun W,Chu M X. 2021. Analysis of expression profiles of CircRNA and MiRNA in oviduct during the follicular and luteal phases of sheep with two fecundity (FecBgene) genotypes[J]. Animals, 11(10):2826. doi:10.3390/ani111 02826.
Liang X L, Luo X L, Lin H X, Han F L, Qin J G, Chen L Q,Xu C, Li E C. 2022. Effects and mechanism of different phospholipid diets on ovary development in female broodstock Pacific white shrimp,Litopenaeus vannamei[J]. Frontiers in Nutrition, 9:830934. doi:10.3389/fnut. 2022.830934.
Liu Q N, Huang L, Wang S Y, Li Y T, Tang Y Y, Zhang D Z,Tang B P, Yang H, He J X, Ding F. 2021. Transcriptome analysis of differentially expressed genes in the red swamp crayfish Procambarus clarkii challenged with Aeromonas hydrophila[J]. Fish & Shellfish Immunology, 119:280-288. doi: 10.1016/j.fsi.2021.09.028.
Liu Q N, Xin Z Z, Liu Y, Wang Z F, Chen Y J, Zhang D Z,Jiang S H, Chai X Y, Zhou C L, Tang B P. 2017. A ferritin gene from Procambarus clarkii, molecular characterization and in response to heavy metal stress and lipopolysaccharide challenge[J]. Fish & Shellfish Immunology, 63:297-303. doi: 10.1016/j.fsi.2017.02.025.
Lokman P M, Kazeto Y, Ijiri S, Young G, Miura T, Adachi S,Yamauchi K. 2003. Ovarian mitochondrial cytochrome b mRNA levels increase with sexual maturity in freshwater eels(Anguilla spp.)[J]. Journal of Comparative Physiology, 173 (1):11-19. doi: 10.1007/s00360-002-0304-x.
Love M I, Huber W, Anders S. 2014. Moderated estimation of fold change and dispersion for RNA-Seq data with DESeq2[J]. Genome Biology, 15(12):550. doi: 10.1186/s13059-014-0550-8.
Luckenbach J A, Yamamoto Y, Guzmán J M, Swanson P. 2013. Identification of ovarian genes regulated by folliclestimulating hormone(Fsh) in vitro during early secondary oocyte growth in coho salmon[J]. Molecular and Cellular Endocrinology, 366(1):38-52. doi:10.1016/j.mce. 2012.11.015.
Ngernsoungnern P, Ngernsoungnern A, Kavanaugh S, Sobhon P, Sower S A, Sretarugsa P. 2008. The presence and distribution of gonadotropin-releasing hormone-liked factor in the central nervous system of the black tiger shrimp,Penaeus monodon[J]. General and Comparative Endocrinology, 155 (3):613-22. doi: 10.1016/j.ygcen.2007.08.012.
Pal A, Pal A, Banerjee S, Batabyal S, Chatterjee P N. 2019.Mutation in Cytochrome B gene causes debility and adverse effects on health of sheep[J]. Mitochondrion, 46:393-404. doi: 10.1016/j.mito.2018.10.003.
Paul J S, Gupta N, Beliya E, Tiwari S, Jadhav S K. 2021. Aspects and recent trends in microbial α-amylase:Areview[J]. Applied Biochemistry and Biotechnology, 193(8):2649-2698. doi: 10.1007/s12010-021-03546-4.
Pertea M, Kim D, Pertea G M, Leek J T, Salzberg S L. 2016.Transcript-level expression analysis of RNA-Seq experiments with HISAT, StringTie and Ballgown[J]. Nature protocols, 11 (9):1650-1667. doi: 10.1038/nprot.2016.095.
Pertea M, Pertea G M, Antonescu C M, Chang T C, Mendell J T, Salzberg S L. 2015. StringTie enables improved reconstruction of a transcriptome from RNA-Seq reads[J].Nature Biotechnology, 33(3):290-295. doi:10.1038/nbt. 3122.
Roychoudhury S, Nath S, Massanyi P, Stawarz R, Kacaniova M, Kolesarova A. 2016. Copper-induced changes in reproductive functions:In vivo and in vitro effects[J]. Physiological Research, 65(1):11-22. doi:10.33549/physiolres. 933063.
Sarojini R, Nagabhushanam R, Fingerman M. 1995. Naphthalene-induced atresia in the ovary of the crayfish, Procambarus clarkii[J]. Ecotoxicology and Environmental Safety, 31 (1):76-83. doi: 10.1006/eesa.1995.1046.
Shen H S, Hu Y C, Ma Y C, Zhou X, Xu Z H, Shui Y, Li C Y, Xu P, Sun X W. 2014. In-depth transcriptome analysis of the red swamp crayfish Procambarus clarkii[J]. PLoS One, 9 (10):e110548. doi: 10.1371/journal.pone.0110548.
Silveyra G R, Silveyra P, Vatnick I, Medesani D A, Rodríguez E M. 2018. Effects of atrazine on vitellogenesis, steroid levels and lipid peroxidation, in female red swamp crayfish Procambarus clarkii[J]. Aquatic Toxicology, 197:136-142. doi: 10.1016/j.aquatox.2018.02.017.
Simão F A, Waterhouse R M, Ioannidis P, Kriventseva E V,Zdobnov E M. 2015. BUSCO:Assessing genome assembly and annotation completeness with single-copy orthologs[J]. Bioinformatics, 31 (19):3210-3212. doi: 10.1093/bioinformatics/btv351.
Sirotkin A V, Radosová M, Tarko A, Martín-García I, Alonso F. 2020. Effect of morphology and support of copper nanoparticles on basic ovarian granulosa cell functions[J].Nanotoxicology, 14(5):683-695. doi:10.1080/17435390. 2020.1736680.
Sittikankaew K, Pootakham W, Sonthirod C, Sangsrakru D,Yoocha T, Khudet J, Nookaew I, Uawisetwathana U, Rungrassamee W,Karoonuthaisiri N. 2020. Transcriptome analyses reveal the synergistic effects of feeding and eyestalk ablation on ovarian maturation in black tiger shrimp[J]. Scientific Reports, 10 (1):3239. doi: 10.1038/s41598-020-60192-2.
Sokolova I M. 2013. Energy-limited tolerance to stress as a conceptual framework to integrate the effects of multiple stressors[J]. Integrative and Comparative Biology, 53 (4):597-608. doi: 10.1093/icb/ict028.
Song B S, Jeong P S, Lee J H, Lee M H, Yang H J, Choi S A,Lee H Y, Yoon S B, Park Y H, Jeong K J, Kim Y H, Jin Y B, Kim J S, Sim B W, Huh J W, Lee S R, Koo D B,Chang K T, Kim S U. 2018. The effects of kinase modulation on in vitro maturation according to different cumulus-oocyte complex morphologies[J]. PLoS One, 13 (10):e0205495. doi: 10.1371/journal.pone.0205495.
Steinhauser C B, Wing T T, Gao H, Li X, Burghardt R C, Wu G, Bazer F W, Johnson G A. 2017. Identification of appropriate reference genes for qPCR analyses of placental expression of SLC7A3 and induction of SLC5A1 in porcine endometrium[J]. Placenta, 52:1-9. doi:10.1016/j.placenta. 2017.02.003.
Tang B, Wang S, Wang S G, Wang H J, Zhang J Y, Cui S Y. 2018. Invertebrate trehalose-6-phosphate synthase gene:Genetic architecture, biochemistry, physiological function,and potential applications[J]. Frontiers in Physiology, 9:30. doi: 10.3389/fphys.2018.00030.
Tian Y, Heng D, Xu K L, Liu W B, Weng X C, Hu X S,Zhang C. 2018. cGMP/PKG-I pathway-mediated GLUT1/4 regulation by NO in female rat granulosa cells[J]. Endocrinology, 159(2):1147-1158. doi:10.1210/en. 2017-00863.
Tingaud-Sequeira A, Chauvigné F, Lozano J, Agulleiro M J,Asensio E, Cerdà J. 2009. New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis[J]. BMC Genomics, 10:434. doi: 10.1186/1471-2164-10-434.
Tinikul Y, Poljaroen J, Nuurai P, Anuracpreeda P, Chotwiwatthanakun C, Phoungpetchara I, Kornthong N, Poomtong T, Hanna P J, Sobhon P. 2011. Existence and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei[J]. Cell and Tissue Research, 343 (3):579-93. doi: 10.1007/s00441-010-1112-3.
Treen N, Itoh N, Miura H, Kikuchi I, Ueda T, Takahashi K G,Ubuka T, Yamamoto K, Sharp P J, Tsutsui K, Osada M. 2012. Mollusc gonadotropin-releasing hormone directly regulates gonadal functions:A primitive endocrine system controlling reproduction[J]. General and Comparative Endocrinology, 176(2):167-72. doi:10.1016/j.ygcen. 2012.01.008.
Tzivion G, Dobson M, Ramakrishnan G. 2011. FoxO transcription factors;Regulation by AKT and 14-3-3 proteins[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1813(11):1938-1945. doi:10.1016/j. bbamcr. 2011.06.002.
Veenstra J A. 2015. The power of next-generation sequencing as illustrated by the neuropeptidome of the crayfish Procambarus clarkii[J]. General and Comparative Endocrinology, 224:84-95. doi: 10.1016/j.ygcen.2015.06.013.
Wan H F, Zhong J Y, Zhang Z P, Sheng Y Z, Wang Y L. 2022. Identification and functional analysis of the doublesex gene in the mud crab Scylla paramamosain[J]. Comparative Biochemistry and Physiology. Part A:Molecular & Integrative Physiology, 266:111150. doi: 10.1016/j.cbpa.2022.111150.
Wang J, Zhou J L, Yang Q, Wang W W, Liu Q, Liu W Z, Liu S Z. 2020. Effects of 17 α-methyltestosterone on the transcriptome, gonadal histology and sex steroid hormones in Pseudorasbora parva[J]. Theriogenology, 155:88-97. doi: 10.1016/j.theriogenology.2020.05.035.
Xie J K, Wang Q, Zhang T T, Yin S, Zhang C L, Ge Z J. 2016. Repeated superovulation may affect mitochondrial functions of cumulus cells in mice[J]. Scientific Reports, 6:31368. doi: 10.1038/srep31368.
Xiong R H, Wen S L, Wang Q, Zhou H Y, Feng S. 2018.Morphological and molecular variations induce mitochondrial dysfunction as a possible underlying mechanism of athletic amenorrhea[J]. Experimental and Therapeutic Medicine, 15 (1):993-998. doi: 10.3892/etm.2017.5469.
Yang H, Ji T W, Xiong H R, Zhang Y Y, Wei W Z, Liu Q N. 2022. Transcriptome profiles of red swamp crayfish Procambarus clarkii hematopoietic tissue in response to WSSV challenge[J]. Fish & Shellfish Immunology, 122:146-152. doi: 10.1016/j.fsi.2022.01.041.
Yin N, Wang Y L, Lu X Y, Liu R R, Zhang L S, Zhao W, Yuan W D, Luo Q Q, Wu H, Luan X Y, Zhang H Q. 2018.hPMSC transplantation restoring ovarian function in premature ovarian failure mice is associated with change of Th17/Tc17 and Th17/Treg cell ratios through the PI3K/Akt signal pathway[J]. Stem Cell Research & Therapy, 9(1):37. doi: 10.1186/s13287-018-0772-x.
Yu S, Meng S M, Xiang M X, Ma H. 2021. Phosphoenolpyruvate carboxykinase in cell metabolism:Roles and mechanisms beyond gluconeogenesis[J]. Molecular Metabolism, 53:101257. doi: 10.1016/j.molmet.2021.101257.
Zhang H, Lin H Y, Yang Q, Wang H X, Chai K X, Chen L M, Zhu C. 2007. Expression of prostasin serine protease and protease nexin-1 (PN-1)in rhesus monkey ovary during menstrual cycle and early pregnancy[J]. Journal of Histochemistry & Cytochemistry,55(12):1237-44. doi: 10.1369/jhc.7A7232.2007.
Zhang M W, Wei H L, Liu T, Li W R, Li Y J, Wang S, Xing Q, Hu X L, Zhang L L, Bao Z M. 2020. Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis[J]. The Journal of Steroid Biochemistry and Molecular Biology, 204:105756. doi:10.1016/j. jsbmb. 2020.105756.
Zhang Y N, Jiang S F, Qiao H, Xiong Y W, Fu H T, Zhang W Y, Gong Y S, Jin S B, Wu Y. 2021. Transcriptome analysis of five ovarian stages reveals gonad maturation in female Macrobrachium nipponense[J]. BMC Genomics, 22(1):510. doi: 10.1186/s12864-021-07737-5.
Zhao D, Zhang X N, Li X F, Ru S G, Wang Y W, Yin J B,Liu D S. 2019. Oxidative damage induced by copper in testis of the red swamp crayfish Procambarus clarkii and its underlying mechanisms[J]. Aquatic Toxicology, 207:120-131. doi: 10.1016/j.aquatox.2018.12.006.
Zheng X C, Zhang L, Jiang W B, Abasubong K P, Zhang C Y, Zhang D D, Li X F, Jiang G Z, Chi C, Liu W B. 2021.Effects of dietary icariin supplementation on the ovary development-related transcriptome of Chinese mitten crab(Eriocheir sinensis)[J]. Comparative Biochemistry and Physiology Part D:Genomics and Proteomics, 37:100756. doi: 10.1016/j.cbd.2020.100756.
Zhong Y Z, Zhao W B, Tang Z S, Huang L M, Zhu X X,Liang X, Yan A F, Lu Z F, Yu Y L, Tang D S, Wang D P, Lu Z L. 2021. Comparative transcriptomic analysis of the different developmental stages of ovary in red swamp crayfish Procambarus clarkii[J]. BMC Genomics, 22 (1):199. doi: 10.1186/s12864-021-07537-x.
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