Genetic diversity analysis of 147 Nymphaea Linn. plants based on SRAP molecular marker
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摘要: 【目的】对147份睡莲属植物的遗传多样性及亲缘关系进行分析，为睡莲属种质资源保护、开发利用及新品种选育的亲本选择提供科学参考。【方法】筛选获得扩增条带清晰、多态性好的SRAP引物，对112份睡莲属植物种质和35份杂交后代进行多态性扩增，基于扩增结果构建0/1矩阵，利用Popgene 1.32计算遗传多样性相关参数。最后采用NTSYS 2.1的非加权组平均法（UPGMA）计算遗传相似系数和遗传距离并构建聚类图。【结果】利用筛选的10个引物对从147份睡莲属植物材料中扩增出207个条带，其中多态性条带207条，多态性比率100%，平均每对引物扩增20.7条。147份睡莲属植物的观测等位基因数（Na）为2.0000，有效等位基因数（Ne）为1.1777~1.3339，平均为1.2535，Shannon信息指数（I）为0.2459~0.3703，平均为0.3155，Nei’s遗传多样性指数（H'）为0.1345~0.2230，平均为0.1835。在遗传相似系数0.65和遗传距离为1.12时，均可将147份睡莲属植物材料划分为六大类，并依据10个引物对扩增的0/1矩阵构建了147份睡莲属植物材料的DNA分子身份证。【结论】睡莲属植物具有丰富的遗传多样性。采用SRAP分子标记可有效鉴定睡莲属植物材料的亲缘关系远近，有助于提高亲本选择率和育种进程。筛选出的10个引物对能有效地鉴定35份杂交后代。Abstract: 【Objective】 To analyze genetic diversity and genetic relationships among 147 Nymphaea Linn. plants，and to provide scientific reference for conservation， development and utilization of Nymphaea Linn. germplasm resources and parents selection for new varieties breeding.【Method】 Ten SRAP primer pairs with clear amplification bands， stable structure and abundant polymorphism were screened to perform polymorphic amplification on 112 Nymphaea Linn. germplasm resources and 35 hybrid progenies. Based on amplification results， a 0/1 matrix was established and genetic diversity indexes were calculated using POPGENE 1.32. Genetic similarity coefficient and genetic distance were calculated through unweighted group average（UPGMA）method of NTSYS 2.1 and cluster diagram was constructed.【Result】 Using the 10 screened primer pairs， 207 polymorphic bands were detected from the 207 amplified bands，indicating a polymorphic rate of 100% and on average every primer pair produced 20.7 polymorphic loci. For 147 Nymphaea Linn. plants，observed number of alleles（Na）was 2.0000 and effective number of alleles（Ne）was from 1.1777 to 1.3339， with an average of 1.2535；Shannon’s information index（I）was from 0.2459 to 0.3703， with an average of 0.3155， and the Nei’s genetic diversity index（H'）was from 0.1345 to 0.2230， with an average of 0.1835. The 147 Nymphaea Linn. plants were divided into 6 groups when similarity coefficient was 0.65 and genetic distance was 1.12. DNA molecular ID of 147 Nymphaea Linn. plants were established according to the 0/1 matrix based on 10 primer pairs.【Conclusion】Nymphaea Linn. plants enjoy rich genetic diversity. With SRAP molecular markers，genetic relationship can be effectively identified，which is helpful for increasing parental selection rate and improving breeding process. And the 10 screened primer pairs can effectively identify 35 hybrid progenies.
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Keywords:
- water lily /
- germplasm resource /
- genetic diversity /
- SRAP molecular marker
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