Effects of resveratrol and EGCG combination on antioxidant capacity of mouse skeletal muscle
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摘要: 【目的】研究白藜芦醇(Resveratrol,RES)和表没食子儿茶素没食子酸酯(Epigallocatechin-3-Gallate, EGCG)联合使用对小鼠骨骼肌抗氧化能力影响的机制,为开发能通过提高畜禽骨骼肌抗氧化能力进而改善肉品质的新型、绿色、安全的饲料添加剂提供理论依据。【方法】将体重18~22 g的清洁级昆明种小鼠40只,随机分为4组,即二甲基亚砜(DMSO)对照组(CK)、RES组、EGCG组和RES+EGCG联用组,每组10只,采取灌胃的方式对应给药,剂量为每天400 mg/kg,连续给药28 d后测定小鼠骨骼肌中抗氧化酶活力指标,并通过荧光定量PCR方法测定小鼠骨骼肌中抗氧化相关基因的表达量。【结果】RES和EGCG联用小鼠的采食量、体重和脏器指数无显著影响(P>0.05)。与CK相比,RES组、EGCG组及RES+EGCG组血清和肌肉中丙二醛(MDA)含量显著降低(P<0.05,下同),谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)活力显著升高,小鼠腓肠肌中GSH-Px、超氧化物歧化酶1(SOD1)、超氧化物歧化酶2(SOD2)和核因子NF-E2相关因子(Nrf2)基因表达水平显著升高,Kelch样环氧氯丙烷相关蛋白1(Keap1)基因表达水平显著降低。与RES或EGCG单独使用组相比,RES+EGCG联用组血清中GSH-Px、T-SOD活力显著升高,MDA含量显著降低,腓肠肌中GSH-Px基因表达水平显著升高;联用组SOD1基因表达水平与EGCG组相比显著升高,SOD2基因表达水平与RES组相比显著升高。【结论】RES和EGCG联用显著提高小鼠肌肉抗氧化酶活力及相关抗氧化基因的表达,并可能激活Keap1-Nrf2信号通路,表明RES和EGCG联用可提高肌肉的抗氧化水平。Abstract: 【Objective】Studying on the mechanism of the combination of resveratrol(RES)and epigallocatechin-3-gallate(EGCG)on the antioxidant capacity of skeletal muscle in mice,in order to develop a theoretical basis for the development of new,green and safe feed additive which could improve meat quality by enhancing the antioxidant capacity of skeletal muscle in livestock and poultry.【Method】Forty cleaning grade Kunming mice weighing 18-22 g were randomly divided into four groups,namely the dimethyl sulfoxide(DMSO)control group,the RES group,the EGCG group and the RES + EGCG combination group. Ten mice in each group were administered by gavage at a dose of 400 mg/kg per day,and the antioxidant enzyme activity indexes were measured in the skeletal muscle of mice after 28 d of continuous administration,and the expression of antioxidant-related genes in the skeletal muscle of mice was determined by fluorescence quantitative PCR.【Result】There was no significant effect on food intake,body weight and organ index in mice treated with RES in combination with EGCG(P>0.05). Compared with DMSO,malondialdehyde(MDA)levels in serum and muscle were significantly lower in the RES,EGCG and RES+EGCG groups(P<0.05,the same below),glutathione peroxidase(GSH-Px)and total superoxide dismutase(T-SOD)activities were significantly higher,GSH-Px,superoxide dismutase 1(SOD1),superoxide dismutase 2(SOD2)and nuclear factor NF-E2-related factor(Nrf2)gene expression levels were significantly increased and Kelch-like ECH associated protein 1(Keap1)gene expression levels were significantly decreased in the gastrocnemius of mice. Compared with RES or EGCG alone,the serum levels of GSH-Px and T-SOD were significantly higher,MDA levels were significantly lower,and GSH-Px gene expression levels were significantly higher in gastrocnemius muscle;the expression levels of SOD1 gene were significantly higher in the combined group compared with the EGCG group,and the expression levels of SOD2 gene were significantly higher compared with that of RES group.【Conclusion】The combination of RES and EGCG significantly increases the activity of antioxidant enzymes and the expression of related antioxidant genes in mice,and may activate the Keap1-Nrf2 signaling pathway,indicating that the combination of RES and EGCG can improve the antioxidant level in muscles.
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Keywords:
- resveratrol /
- EGCG /
- skeletal muscle /
- antioxidant /
- Nrf2 signaling pathway
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