生姜主栽品种枯萎病抗性评价及抗病性鉴定指标筛选

Identification and evaluation of resistance to fusarium wilt of ginger varieties and screening of resistance indexes

  • 摘要: 【目的】筛选抗枯萎病的生姜品种,建立抗病评价体系,为生姜枯萎病抗病品种选育及抗病机理研究提供理论参考。【方法】选取5个生姜主产区的主栽品种,采用室内人工接种法测定枯萎病菌(腐皮镰刀菌)侵染对不同生姜品种超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、几丁质酶(CHI)、β-1,3-葡聚糖苷酶(GLU)活性及次生抗菌物质含量、超氧阴离子(O2-·)、过氧化氢(H2O2)、丙二醛(MDA)含量等的影响,结合抗病系数、主成分分析、聚类分析、相关分析和隶属函数对5个生姜品种幼苗的抗病性进行综合评价。【结果】山东大姜病情指数最低(4.0)、发病率最低(4%)、发病周期最长(24 d),表现出较强的抗病性;罗平小黄姜病情指数最高(45.2)、发病周期最短(7 d),表现抗病性较弱。不同生姜品种幼苗接菌后的生理生化指标均出现不同程度的变化,其中,山东大姜的抗氧化酶(SOD、POD、CAT)活性、防御酶(PAL、CHI、GLU)活性和次生抗菌物质(总酚、类黄酮、木质素)含量均高于其他品种,O2-·、H2O2和MDA含量低于其他品种,而罗平小黄姜呈现相反趋势。主成分分析将12个单项生理生化指标转换为3个独立综合指标,聚类分析将5个生姜品种划分为3类,结合隶属函数综合评价结果,5个生姜品种抗病性强弱表现为:山东大姜>凤头姜>竹根姜>贵州黄姜>罗平小黄姜,与人工接种试验中5个品种的发病程度一致。相关分析结果显示,SOD和类黄酮与抗病性综合评价值(D值)呈极显著正相关(P<0.01)。【结论】5个生姜品种中山东大姜抗病性最强,罗平小黄姜抗病性最弱;SOD和类黄酮可作为生姜枯萎病抗性鉴定的主要参考指标。

     

    Abstract: 【Objective】To screen ginger varieties resistant to fusarium wilt and establish disease resistance evaluation system,so as to provide theoretical reference for the research on disease-resistant variety breeding of ginger and theory of disease resistance mechanism.【Method】Five main ginger varieties in the main producing areas were selected,and the effects of fusarium wilt pathogen(Fusarium solani)infection on superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),phenylalaninammo-nialyase(PAL),chitinase(CHI),β-1,3-glucanase(GLU)enzyme activities,secondary antibacterial substances content,superoxide(O2-·),hydrogen peroxide(H2O2),and malondialdehyde(MDA)content of different ginger varieties were determined by indoor artificial inoculation method. The disease resistance of five ginger varieties at seedling stage was comprehensively evaluated by combining disease resistance coefficient,principal component analysis,cluster analysis,correlation analysis and membership function.【Result】The results showed that the disease index of Shandong ginger was the lowest(4.0),the incidence rate was the lowest(4%),and the disease cycle was the longest(24 d),showing strong disease resistance. The disease index of Luoping small yellow ginger was the highest(45.2),and the disease cycle was the shortest(7 d),showing weak disease resistance. Physiological indexes of different ginger varieties after inoculation showed different changes. Antioxidant enzyme(SOD,POD and CAT)activity,defensive enzyme(PAL,CHI and GLU)activity and secondary antibacterial substances(total phenols,flavonoids,lignin)content in Shandong ginger were higher than those of other varieties,and the O2-·,H2O2 and MDA content in Shandong ginger were lower than those of other varieties. Luoping small yellow ginger showed the opposite trend. Twelve individual physiological indexes were transformed into three independent comprehensive indexes by principal component analysis,and five ginger varieties were divided into three categories by cluster analysis. Taking comprehensive evaluation results of membership function into account,the order of disease resistance of five main varieties from strong to weak showed as: Shandong ginger>Fengtou ginger>Zhugen ginger>Guizhou yellow ginger>Luoping small yellow ginger. The ranking results were consistent with the results of artificial inoculation identification of disease resistance. Correlation analysis showed that SOD had an extremely significant positive correlation with flavonoids and comprehensive evaluation value of disease resistance(D value)(P<0.01).【Conclusion】Shandong ginger has the strongest disease resistance among the five ginger varieties,and Luoping small yellow ginger has the worst disease resistance. SOD and flavonoid can be used as the main reference indexes for the identification of ginger disease resistance.

     

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