Abstract: 【Objective】 To explore tissue expression characteristics of TLRP gene（PmTLRP）in Pinctada fucata martensii and temporal expression of PmTLRP under stimulations of Vibrio harveyi，nucleus insertion operation and lipopolysaccharide（LPS），so as to provide a foundation for further exploring immune mechanism of TLRs in P.fucata martensii.【Method】cDNA sequences of PmTLRP gene was cloned by rapid amplification of cDNA ends（RACE），and bioinformatics analysis was carried out by online software such as ProtParam，ProtScale，SignalP 4.0，TMHMM v.2.0，SMART，SoftBerry Psite and SOPMA.Quantitative real-time PCR（qRT-PCR）was used to detect the expression of PmTLRP gene in various tissues of P.fucata martensii and under stimulations of V.harveyi stimulation，nucleus insertion operation and LPS stimulation.【Result】 The total length of PmTLRP cDNA was 2214 bp，including a 5'-UTR of 33 bp，a 3'-UTR of 135 bp and an open reading frame（ORF）of 2043 bp which encoded 681 amino acid residues.The protein encoded had signal peptide，leucine rich repeats（LRRs），transmembrane domain，and TIR domain，which was consistent with the characteristics of TLRs family.Amino acid sequences of PmTLRP were somehow similar with that of TLRs from other species，with the highest similarity with amino acid sequences of TLP in Crassostrea virginica.PmTLRP was expressed in mantle，hemolymph，hepatopancreas，gills，gonads and adductor muscle，and the highest expression was found hepatopancreas，which was significantly higher than that in other tissues（P<0.05）.After stimulation of V.harveyi，expression of PmTLRP began to increase at 12 h，reached the highest expression at 16 h，about 19.0 times that of the control group before stimulation（0 h）.Under stimulation of nucleus insertion operation，the relative expression of PmTLRP gene increased from 5 to 20 d.Finally，it reached the maximum expression at 30 d，which was about 6.4 times that of the control group.After LPS stimulation，the expression of PmTLRP in hemolymph was significantly up-regulated，and reached the maximum at 3 h，about 5.0 times that of the blank control group（0 h）.PmTLRP in hepatopancreas showed a fluctuating trend of increase→decrease→increase→decrease→increase，and the relative expression increased to the maximum at 24 h after injection stimulation，which was about 6.0 times that of the control group.【Conclusion】 PmTLRP gene is expressed in mantle，hemolymph，hepatopancreas，gill，gonad and adductor muscle of P.fucata martensii，but the expression are different.The expression of PmTLRP was obviously up-regulated after stimulations of V.harveyi，nucleus insertion operation and LPS，indicating that TLRP plays an important role in the immune defense response of P.fucata martensii.