Abstract: 【Objective】To study effects of adding single and multiple lactic acid bacteria on Sorghum bicolor×Sorghum sudanense silage, so as to provide scientific basis for the development of silage agents.【Method】Lactobacillus plantarum(LP06), Pediococcus pentosaceus(PP02) and L. brevis(LBR02) selected from grass were added to S. bicolor×S. sudanense for silage preparation with amount of 5×10⁵ CFU/g, and 8 treatments were designed:(1) CK(no lactic acid); (2) LP06;(3) PP02;(4) LBR02;(5) F-1(LP06:PP02=1:1);(6) F-2(LP06:LBR02=1:1);(7) F-3(PP02:LBR02=1:1); (8) F-4(LP06:PP02:LBR02=1:1:1). The sensory quality, fermentation quality, nutritional ingredient and major microbial quantity of 8 treatments were analyzed after 60 d of silage with comprehensive evaluation by membership function method.【Result】After 60 d silage, the sensory quality of LP06 and F-1 treatments was with aromatic fruit odor and the best sensory quality. The lowest pH value was in F-2 treatment, 3.88. NH₃-N contents of 7 lactic acid addition treatments were significantly lower than those of CK(P<0.05, the same below). The highest lactic acid content was in LP06 treatment, 4.21%. Acetic acid content of LBR02, F-2, F-3, F-4 treatments was significantly higher than those in other treatments. LP06, F-1 and F-4 treatments had the highest quantity of lactic acid bacteria, all above 7.00×10⁵ CFU/g. No yeasts and molds were detected in LBR02, F-2 and F-3 treatments, and the growth inhibition effect of harmful microorganisms was significant. The CP content of 7 lactic acid addition treatments was significantly higher than that of CK, while the water-soluble carbohydrate(WSC) content of those treatments was significantly lower than that of CK. The contents of dry matter(DW), acid detergent fiber(ADF) and neutral detergent fiber(NDF) had no significantly different among 8 treatments(P>0.05). According to the comprehensive evaluation, F-2 treatment's membership function value was the highest (0.69), and F-2 treatment showed the best silage effects.【Conclusion】Homofermentative lactic acid bacteria LP06 have more advantages in improving S. bicolor×S. sudanense silage sensory quality, reducing NH₃-N content and CP loss, increasing lactic acid content and lactic acid bacteria quantity, while heterofermentative lactic bacteria LBR02 have more significant effects on inhibiting harmful microorganism growth. LP06 and LBR02 mixed with 1:1 have the best comprehensive performance of silage effects.