Abstract: 【Objective】To analyze the genetic diversity of main agronomic traits of ginger germplasm resources and screen out the excellent core germplasm resources，so as to provide theoretical reference for the innovative utilization of ginger germplasm resources and cultivar selection in karst areas.【Method】Ninety-six ginger germplasm resources from different provinces（regions）in China were taken as materials and their 10 main agronomic traits were determined for 2 consecutive years from 2018 to 2019 in Guizhou.The genetic variation analysis，correlation analysis，principal component analysis，Ward method cluster analysis and comprehensive evaluation were conducted to evaluate genetic diversity of these 96 ginger germplasm resources.【Result】Coefficients of variation of 10 agronomic traits of 96 ginger germplasm resources ranged from 6.77% to 36.64%，and the coefficients of variation for branch number，stem and leaf weight and rhizomes weight were higher than 20.00%.Genetic diversity index（H'）ranged from 1.935 to 2.099.The H'of leaf width，leaf length and rhizome length were higher，and the H'of branch number was the smallest（1.935）.Rhizomes weight per plant was positively correlated with branch number，stem and leaf weight，rhizome length and rhizome width（P<0.01），but had no significant correlation with plant height，number of main stem leaves，main stem diameter，leaf length and leaf width（P>0.05）.The accumulative contribution rate of the first 3 principal component factors was 63.48%，and their loadings were significantly different among the traits，indicating that the variation of ginger germplasm resources was caused by various reasons.Cluster analysis showed that 96 ginger germplasm resources were divided into 6 groups at a genetic distance of 10.5.Among them，the germplasm resources of group I，group II and group V had heavier stems and leaves and higher rhizome yield，while the others had fewer branches，lighter stems and leaves and lower rhizome yield.The genetic background of ginger germplasm population in Guizhou was close to those of Guangxi，Hubei and Sichuan populations.【Conclusion】 The genetic diversity of main agronomic traits of the 96 germplasm resources of ginger is rich.Branch number and stem and leaf weight are the main reasons for the high yield of ginger in karst area.The local resources of ginger originate from Guangxi，Hubei and Sichuan are suitable be popularized and planted in Guizhou ecological area.The 14 germplasm with excellent comprehensive agronomic traits selected from group I，II and V can be used as core germplasm resources for development and utilization.