Abstract: 【Objective】To analyze physiological characteristics of representative wild tea resources lines（green bud line and purple bud line）in Zhenfeng County，Guizhou province，so as to provide references for screening Guizhou wild tea population resources with comprehensive resistance and abundant substance.【Method】Taking Fuding-dabaicha and Longjing 43 as controls，twenty-two morphological characteristics and leaf anatomical structure indexes，including bud width，leaf area，upper epidermis thickness，spongy tissue thickness and main vein thickness were measured to access genetic diversity，comprehensive resistance and internal subsistance of purple-bud tea tree lines and green-bud tea tree lines comprehensive resistance.【Result】 Zhenfeng wild tea trees had plump buds，dense trichomes.It was a large leaf tea variety with a leaf surface areas between 40.27-52.87 cm²，multiple leaf colors，including purple，purple-green and deep green and its leaves were soft with lustre.The internode was as long as 5.14 cm，and numbers of leaf veins were between 10 and 12.The representative indexes of Zhenfeng wild tea tree related to resistance and internal subsistence were:the main vein thickness of leaves was 704.71-740.02 μm；the upper epidermis thickness was 20.33-21.72 μm；the bud width was 0.30-0.36 cm；spongy tissue thickness was 81.05-84.31 μm.Such representative indexes of Zhenfeng wild tea trees were obviously higher than that of cultivated tea trees in control groups.Principal component analysis showed the genetic distance between Zhenfeng wild tea trees and green-bud，purple-bud lines was closer and genetic distance between Zhenfeng wild tea trees and cultivated tea trees was longer.Membership function analysis showed that the order of the mean membership function value was： Green bud line>purple bud line>Fuding-dabaicha>Longjing 43.The indexes of comprehensive resistance and components of green-bud line were 0.4743 and 0.4599，and were 0.4618 and 0.4325 of the purple-bud line，respectively，which were higher than those of controls.【Conclusion】Comprehensive resistance，internal subsistance and adaptability of Zhenfeng wild tea trees are better than those of Fuding Dabaicha and Longjing 43.Comprehensive quality of green-bud line is better than purple-bud line，indicating that green-bud line can be taken as high-quality material of new variety breeding and genetic improvement.