淡水养殖凡纳滨对虾生长相关基因HDAC1克隆及其表达分析

骆永丽, 刘红, 于忠利, 王文雁, 戴习林

骆永丽, 刘红, 于忠利, 王文雁, 戴习林. 2022: 淡水养殖凡纳滨对虾生长相关基因HDAC1克隆及其表达分析. 南方农业学报, 53(5): 1388-1398. DOI: 10.3969/j.issn.2095-1191.2022.05.021
引用本文: 骆永丽, 刘红, 于忠利, 王文雁, 戴习林. 2022: 淡水养殖凡纳滨对虾生长相关基因HDAC1克隆及其表达分析. 南方农业学报, 53(5): 1388-1398. DOI: 10.3969/j.issn.2095-1191.2022.05.021
LUO Yong-li, LIU Hong, YU Zhong-li, WANG Wen-yan, DAI Xi-lin. 2022: Cloning and expression analysis of HDAC1 gene related to growth of Litopenaeus vannamei cultured in freshwater. Journal of Southern Agriculture, 53(5): 1388-1398. DOI: 10.3969/j.issn.2095-1191.2022.05.021
Citation: LUO Yong-li, LIU Hong, YU Zhong-li, WANG Wen-yan, DAI Xi-lin. 2022: Cloning and expression analysis of HDAC1 gene related to growth of Litopenaeus vannamei cultured in freshwater. Journal of Southern Agriculture, 53(5): 1388-1398. DOI: 10.3969/j.issn.2095-1191.2022.05.021

淡水养殖凡纳滨对虾生长相关基因HDAC1克隆及其表达分析

基金项目: 

上海市科技兴农项目(2019-02-08-00-F01111)

详细信息
    作者简介:

    骆永丽(1995-),https://orcid.org/0000-0001-8302-4195,研究方向为凡纳滨对虾繁殖及发育生物学,E-mail:2605203817@qq.com

    通讯作者:

    刘红(1969-),https://orcid.org/0000-0003-2519-5214,博士,副教授,主要从事凡纳滨对虾良种繁育研究工作,E-mail:hliu@shou.edu.cn

  • 中图分类号: S966.12

Cloning and expression analysis of HDAC1 gene related to growth of Litopenaeus vannamei cultured in freshwater

Funds: 

Shanghai Science and Technology Promoting Agriculture Project(2019-02-08-00-F01111)

  • 摘要: 【目的】分析组蛋白去乙酰基酶1基因(HDAC1)在不同淡水生长特性家系凡纳滨对虾中的表达情况,明确HDAC1基因在对虾淡水养殖过程中的功能作用,为揭示凡纳滨对虾生长调控的分子机制提供参考依据。【方法】通过RACE克隆凡纳滨对虾HADC1基因cDNA序列,利用DNAMAN、ProtParam、ProtScale、PSORT等在线软件进行生物信息学分析,以实时荧光定量PCR检测凡纳滨对虾HDAC1基因组织表达特征;选取4个家系凡纳滨对虾在1‰盐度下进行淡水养殖,养殖70 d后统计对虾存活率(RS)、日增重率、体长日增率、特定生长率(SGR)及饲料转化率(FCR),并利用实时荧光定量PCR检测凡纳滨对虾肠道中的HDAC1基因表达情况。【结果】凡纳滨对虾HDAC1基因cDNA全长3230 bp,包括67 bp的5'非编码区(5'-UTR)、1711 bp的3'非编码区(3'-UTR)及1452 bp的开放阅读框(ORF),共编码483个氨基酸残基;HDAC1基因DNA全长4692 bp,含有8个外显子和7个内含子。凡纳滨对虾HDAC1基因编码蛋白分子量为54640.22 Da,理论等电点(pI)为5.59,属于稳定的亲水性蛋白,具有1个Histone deacetylase 1结构域,无跨膜结构。凡纳滨对虾HDAC1氨基酸序列与斑节对虾HDAC1氨基酸序列的相似性最高,达99.79%。HDAC1基因在凡纳滨对虾各组织中均有表达,以在肠道中的相对表达量显著高于在其他组织中的相对表达量(P<0.05);在4个家系群体中,无论是大虾还是小虾,生长慢的群体中HDAC1基因相对表达量较低,生长快的群体中HDAC1基因相对表达量较高。【结论】HDAC1基因在凡纳滨对虾各组织中均有表达,以肠道中的相对表达量最高,且HDAC1基因相对表达量与凡纳滨对虾的生长速度成正比,故推测其参与凡纳滨对虾的生长调节过程。
    Abstract: 【Objective】To analyze the expression of histone deacetylase 1 gene(HDAC1)in shrimp families with different freshwater growth characteristics,and to clarify the function of HDAC1 gene related to growth of Litopenaeus vannamei cultured in freshwater,so as to provide a reference for revealing the molecular mechanism of growth regulation of L. vannamei.【Method】The cDNA sequence of HDAC1 gene of L. vannamei was cloned by RACE,and bioinformatics analysis was carried out by using online software such as DNAMAN,ProtParam,Protscale and PSORT. The tissue expression characteristics of HDAC1 gene of L. vannamei were detected by real-time fluorescent quantitative PCR. Four families of L. vannamei were cultured in fresh water at 1‰ salinity. After 70 days of culture,the survival rate(RS),daily gain rate,daily gain rate of body length,specific growth rate(SGR)and feed conversion rate(FCR)of L. vannamei were counted,and the expression of HDAC1 gene in the intestine of L. vannamei was detected by real-time fluorescent quantitative PCR.【Result】The full length of HDAC1 gene cDNA of L. vannamei was 3230 bp,including 67 bp 5'-UTR, 1711 bp 3'-UTR and 1452 bp open reading frame(ORF),encoding 483 amino acids in total. The total length of HDAC1 gene DNA was 4692 bp,containing eight exons and seven introns. The HDAC1 gene of L. vannamei encoded a protein with a molecular weight of 54640.22 Da and a theoretical isoelectric point of 5.59,and it was a stable hydrophilic protein with a histone deacetylase 1 domain with no transmembrane structure. The similarity of HDAC1 amino acid sequence between L. vannamei and Penaeus monodon was the highest,up to 99.79%. HDAC1 gene was expressed in all tissues of L. vannamei,and the relative expression in intestine was significantly higher than that in other tissues(P<0.05). Among the four pedigree populations,whether big shrimp or small shrimp,the relative expression of HDAC1 gene was lower in the slow-growing population,and higher in the fast-growing population.【Conclusion】HDAC1 gene is expressed in all tissues of L. vannamei,with the highest relative expression in the intestine,and the relative expression of HDAC1 gene is directly proportional to the growth rate of L. vannamei,so it is speculated that it is involved in the growth regulation process of L. vannamei.
  • 陈田聪,谢达祥,陈晓汉.2018.南美白对虾淡水养殖常见病害及防治措施[J].南方农业,12(20):145-147.[Chen T C,Xie D X,Chen X H.2018.Common diseases and control measures in freshwater culture of Litopenaeus vannamei[J].South China Agriculture,12(20):145-147.]doi: 10.19415/j.cnki.1673-890x.2018.20.074.
    戴耀达,康顺元,姚翠鸾.2018.凡纳滨对虾苗种产业的存在问题与对策[J].农业开发与装备,(6):7.[Dai Y D,Kang S Y,Yao C L.2018.Problems and countermeasures of Litopenaeus vannamei seed industry[J].Agricultural Development and Equipments,(6):7.]doi: 10.3969/j.issn.1673-9205.2018.06.006.
    樊慧敏,马倩倩,商泽昊,张子涵,董昭然,唐庆权,许晓牧,王黎明,朱若林,鲍传和,彭开松,万全.2019.工厂化淡水养殖凡纳滨对虾甲壳溃疡症病因与防治研究[J].生物学杂志,36(3):65-69.[Fan H M,Ma Q Q,Shang Z H,Zhang Z H,Dong Z R,Tang Q Q,Xu X M,Wang L M,Zhu R L,Bao C H,Peng K S,Wan Q.2019.Study on the cause and control of Carapace ulcer of Litopenaeus vannamei in factory freshwater aquaculture[J].Journal of Biology,36(3):65-69.]doi: 10.3969/j.issn.2095-1736.2019.03.065.
    方振朋,孟宪红,李旭鹏,栾生,曹家旺,陈宝龙,孔杰,闫茂仓,胡利华.2020.基于微卫星分子标记的凡纳滨对虾商业苗种遗传多样性分析[J].渔业科学进展,41(5):101-109.[Fang Z P,Meng X H,Li X P,Luan S,Cao J W,Chen B L,Kong J,Yan M C,Hu L H.2020.Genetic diversity analysis of domestic commercial brands seedlings of Litopenaeus vannamei based on microsatellite molecular markers[J].Progress in Fishery Sciences,41(5):101-109.]doi: 10.19663/j.issn2095-9869.20190527004.
    高晓艳,董迎辉,姚韩韩,林志华.2016.文蛤HDAC1基因克隆、时空表达及生长相关SNP位点筛查[J].水生生物学报,40(3):601-608.[Gao X Y,Dong Y H,Yao H H,Lin Z H.2016.Cloning,spatiotemporal expression and SNPs identification of HDAC1 gene in hard clam Meretrix meretrix[J].Acta Hydrobiologica Sinica,40(3):601-608.]doi: 10.7541/2016.81.
    李慧,徐义平,鲍宝龙.2014.牙鲆胚后发育阶段HDAC1基因的空间表达[J].上海海洋大学学报,23(5):641-648.[Li H,Xu Y P,Bao B L.2014.Different expression pattern of HDAC1 before and during the process of metamorphosis in Paralichthys olivaceus[J].Journal of Shanghai Ocean University,23(5):641-648.]
    李明杨,叶泽坤,杨华,聂光伟,毛元良,毛慧敏,王国水,左波.2017.大白猪群中HDAC1基因多态性与重要经济性状的相关性研究[J].猪业科学,34(10):104-106.[Li M Y,Ye Z K,Yang H,Nie G W,Mao Y L,Mao H M,Wang G S,Zuo B.2017.Study on the correlation between HDAC1 gene polymorphism and important economic traits in large white pigs[J].Swine Industry Science,34(10):104-106.]doi: 10.3969/j.issn.1673-5358.2017.10.027.
    李强勇,李旻,曾地刚,朱威霖,彭敏,杨春玲,刘青云,赵永贞,陈秀荔,陈晓汉.2020.凡纳滨对虾微卫星分子标记的开发及不同养殖家系遗传多态性分析[J].南方农业学报,51(2):429-436.[Li Q Y,Li M,Zeng D G,Zhu W L,Peng M,Yang C L,Liu Q Y,Zhao Y Z,Chen X L,Chen X H.2020..Development of microsatellite markers of Litopenaeus vannamei and genetic polymorphism analysis of different cultured families[J].Journal of Southern Agriculture,51(2):429-436.]doi: 10.3969/j.issn.2095-1191.2020.02.024.
    刘峰.2005.HDAC1基因、PIT-1基因与猪部分经济性状相关性研究[D].长沙:湖南农业大学.[Liu F.2005.Study on effects of HDAC1 gene and PIT-1 gene on partial economic traits in pigs[D].Changsha:Hunan Agricultural University.]
    陆颖,陈琪,张书强,胡楠,徐绘.2020.HDACs在斑马鱼视网膜再生中的作用研究[J].南通大学学报(医学版),40(4):295-299.[Lu Y,Chen Q,Zhang S Q,Hu N,Xu H.2020.A functional study of HDACs during retina regeneration in zebrafish[J].Journal of Nantong University (Medical Science),40(4):295-299.]doi: 10.16424/j.cnki.cn32-1807/r.2020.04.001.
    蒙娟,杨捷,彭梦笛,贾桂霞,何恒斌.2020.东方百合'索邦'组蛋白去乙酰化酶基因LoSorHDA1的克隆与表达分析[J].西北农业学报,29(2):315-325.[Meng J,Yang J,Peng M D,Jia G X,He H B.2020.Cloning and expression analysis of histone deacetylase LoSorHDA1 in lilium oriental hybrid'Sorbonne'[J].Acta Agriculturae Boreali-occidentalis Sinica,29(2):315-325.]doi: 10.7606/j.issn.1004-1389.2020.02.018.
    任付真,姚韩韩,董迎辉,周小龙,林志华.2016.泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析[J].海洋学报,38(8):72-81.[Ren F Z,Yao H H,Dong Y H,Zhou X L,Lin Z H.2016.cDNA,introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa[J].Journal of Oceanology,38(8):72-81.]doi: 10.3969/j.issn.0253-4193.2016.08.008.
    史建磊,熊自立,李涛,张海利,宰文珊.2020.番茄组蛋白乙酰转移酶(HAT)的全基因组鉴定与分析[J].江苏农业学报,36(3):666-674.[Shi J L,Xiong Z L,Li T,Zhang H L,Zai W S.2020.Genome-wide identification and analysis of histone acetyltransferase (HAT) in tomato[J].Jiangsu Journal of Agricultural Sciences,36(3):666-674.]doi: 10.3969/j.issn.1000-4440.2020.03.019.
    唐扬,孟小菲,沈瑞福,黄永春.2018.凡纳滨对虾家系选育的研究与应用[J].水产科学,37(4):555-563.[Tang Y,Meng X F,Shen R F,Huang Y C.2018.Research and application of family selective breeding in culture of Pacific white shrimp Litopenaeus vannamei[J].Fishery Sciences,37(4):555-563.]doi: 10.16378/j.cnki.1003-1111.2018.04.020.
    熊建华,赵永贞,高永华,谢达祥,张彬,陈晓汉.2011.凡纳滨对虾良种培育研究进展[J].南方农业学报,42(5):556-561.[Xiong J H,Zhao Y Z,Gao Y H,Xie D X,Zhang B,Chen X H.2011.Advances in breeding improved varieties of Litopenaeus vannamei[J].Journal of Southern Agriculture,42(5):556-561.]doi: 10.3969/j.issn.2095-11912011.05.025.
    徐婷婷,邵建彬,范文鲁,彭开松,鲍传和.2019.凡纳滨对虾土塘淡水养殖技术模式探析[J].现代农业科技,(18):174-177.[Xu T T,Shao J B,Fan W L,Peng K S,Bao C H.2019.Discussion on technical model of freshwater aquaculture of Litopenaeus vannamei in soil pond[J].Modern Agricultural Science and Technology,(18):174-177.]doi: 10.3969/j.issn.1007-5739.2019.18.101.
    杨颖,陆江,李湘萍,佘纳,唐核心,石德顺,吕祁峰,徐照学.2018.中国南阳黄牛HDAC1基因SNP检测及与生长性状的相关性分析[J].江苏农业科学,46(16):25-30.[Yang Y,Lu J,Li X P,She N,Tang H X,Shi D S,LüQ F,Xu Z X.2018.SNP detection of HDAC1 gene of Chinese Nanyang yellow cattle and correlation analysis with growth traits[J].Jiangsu Agricultural Sciences,46(16):25-30.]doi: 10.15889/j.issn.1002-1302.2018.16.006.
    张宁.2010.蛋白质Beta折叠的分析与预测及生物信息工具开发[D].天津:南开大学.[Zhang N.2010.Analysis and prediction of beta-sheet structures in proteins and bioinformatics software tools developing[D].Tianjin:Nankai University.]doi: 10.7666/d.y1849300.
    张振超,曾安,韩晓帅,李永芹,荆清.2013.HDAC1基因决定涡虫成体干细胞的维持[J].上海交通大学学报(医学版),33(9):1202-1208.[Zhang Z C,Zeng A,Han X S,Li Y Q,Jing Q.2013.HDAC1 determines adult stem cell maintenance in planarian[J].Journal of Shanghai Jiaotong University (Medical Science),33(9):1202-1208.]doi: 10.3969/j.issn.1674-8115.2013.09.004.
    张宗锋.2012.以湛江、茂名海南为例探究我国凡纳滨对虾、罗非鱼养殖现状及面临的问题[D].上海:上海海洋大学.[Zhang Z F.2012.Taking Zhanjiang and Maoming Hainan in China for examples to explore Litopenaeus vannamei's,and tilapia's culture status and faced problems[D].Shanghai:Shanghai Ocean University.]

    Brunmeir R,Lagger S,Seiser C.2009.Histone deacetylase 1 and 2-controlled embryonic development and cell differentiation[J].International Journal of Developmental Biology,53(2-3):275-289.doi: 10.1387/ijdb.082649rb.

    Das P,Bhadra M P.2020.Histone deacetylase (Rpd3) regulates Drosophila early brain development via regulation of Tailless[J].Open Biology,10(9):200029.doi: 10.1098/rsob.200029.

    Fan L F,Wang Z L,Chen M S,Qu Y X,Li J Y,Zhou A G,Xie S L,Zeng F,Zou J X.2019.Microbiota comparison of Pacific white shrimp intestine and sediment at freshwater and marine cultured environment[J].Science of the Total Environment,657:1194-1204.doi: 10.1016/j.scitotenv.2018.12.069.

    Kelly R D W,Cowley S M.2013.The physiological roles of histone deacetylase (HDAC)1 and 2:Complex co-stars with multiple leading parts[J].Biochemical Society Transactions,41(3):741-749.doi: 10.1042/BST20130010.

    Kim C H,An M J,Kim D H,Kim J W.2017.Histone deacetylase 1(HDAC1) regulates retinal development through a PAX6-dependent pathway[J].Biochemical and Biophysical Research Communications,482(4):735-741.doi: 10.1016/j.bbrc.2016.11.103.

    Kim M Y,Yan B W,Huang S M,Yi Q.2020.Regulating the regulators:The role of histone deacetylase 1(HDAC1) in erythropoiesis[J].International Journal of Molecular Sciences,21(22):8460.doi: 10.3390/ijms21228460.

    Kiyooka M,Shimizu Y,Ohshima T.2020.Histone deacetylase inhibition promotes regenerative neurogenesis after stab wound injury in the adult zebrafish optic tectum[J].Biochemical and Biophysical Research Communications,529(2):366-371.doi: 10.1016/j.bbrc.2020.06.025.

    Ko S,Russell J O,Tian J M,Gao C,Kobayashi M,Feng R L,Yuan X D,Shao C,Ding H G,Poddar M,Singh S,Locker J,Weng H L,Monga S P,Shin D H.2019.HDAC1 regulates differentiation of bipotent liver progenitor cells during regeneration via Sox9b and Cdk8[J].Gastroenterology,156(1):187-202.doi: 10.1053/j.gastro.2018.09.039.

    Lu D F,Yao Y,Su Z Z,Zeng Z H,Xing X W,He Z Y,Zhang C X.2017.Downregulation of HDAC1 is involved in the cardiomyocyte differentiation from mesenchymal stem cells in a myocardial microenvironment[J].PLoS One,9(4):e93222.doi: 10.1371/journal.pone.0093222.

    Montgomery R L,Davis C A,Potthoff M J,Haberland M,Fielitz J,Qi X X,Hill J A,Richardson J A,Olson E N.2007.Histone deacetylases 1 and 2 redundantly regulate cardiac morphogenesis,growth,and contractility[J].Genes&Development,21(14):1790-1802.doi: 10.1101/gad.1563807.

    Qiu Y,Zhao Y M,Becker M,John S,Parekh B S,Huang S M,Hendarwanto A,Martinez E D,Chen Y,Lu H X,Adkins N L,Stavreva D A,Wiench M,Georgel P T,Schiltz R L,Hager G L.2006.HDAC1 acetylation is linked to progressive modulation of steroid receptor-induced gene transcription[J].Molecular Cell,22(5):669-679.doi: 10.1016/j.molcel.2006.04.019.

    Tou L Q,Liu Q,Shivdasani R A.2004.Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases[J].Molecular and Cellular Biology,24(8):3132-3139.doi: 10.1128/MCB.24.8.3132-3139.2004.

    Wang Z H,Lyu J L,Wang F,Miao C,Nan Z,Zhang J Y,Xi Y M,Zhou Q,Yang X H,Ge W Z.2018.The histone deacetylase HDAC1 positively regulates Notch signaling during Drosophila wing development[J].Biology Open,7(2):bio029637.doi: 10.1242/bio.029637.

  • 期刊类型引用(2)

    1. 傅铄,卓宏标,刘建勇. 凡纳滨对虾分子育种研究进展. 中国水产科学. 2024(03): 368-379 . 百度学术
    2. 贺莹,李云,刘红. 凡纳滨对虾CCT6基因克隆及SNP位点筛选. 南方农业学报. 2023(09): 2571-2583 . 本站查看

    其他类型引用(0)

计量
  • 文章访问数:  936
  • HTML全文浏览量:  2
  • PDF下载量:  6
  • 被引次数: 2
出版历程
  • 收稿日期:  2021-06-14
  • 刊出日期:  2022-05-24

目录

    /

    返回文章
    返回