Abstract: 【Objective】To clarify the differences between cultivable bacterial communities and populations in the intestinal of Spodoptera frugiperda larvae in different geographical populations in Yunnan,so as to provide theoretical basis for the intestinal bacterial diversity and biological function of S. frugiperda and lay a foundation for the analysis of the environmental adaptation mechanism of S. frugiperda.【Method】Cultivable bacteria in the intestinal of S. frugiperda from four geographical populations,namely,Lancang in Pu'er city,Longyang in Baoshan City,Shilin in Kunming City,and Kaiyuan in Honghe City of Yunnan were isolated and cultured,and the species of bacteria were identified using the morphological and 16S rDNA sequence.【Result】From the intestines of larval S. frugiperda from four geographical populations in Yunnan,a total of 27 bacterial strains of different shapes,colors and sizes were isolated,and 27 bacterial strains belonged to 4 phyla,9 families,12 genera and 19 species. Predominant phyla were Proteobacteria and Firmicutes and the predominant genera was Enterococcus. Among them,the Lancang populations were isolated to 9 bacteria form 8 species,3 phyla,4 families,6 genera;the Shilin populations were isolated to 6 bacteria form 3 phyla,5 families,6 genera and6 species;the Kaiyuan populations were isolated to 5 bacteria form 2 phyla,3 families,4 genera and 5 species;and the Baoshan populations were isolated to 7 bacteria form 3 phyla,6 families,6 genera and 6 species. Enterococcus and Klebsiella from four populations were the common genera. Richness,Shannon,Simpson and pielou evenness indices of four populations were 0.705-1.277,0.969-1.381,0.615-0.635 and 0.602-0.753,respectively.【Conclusion】Cultivable bacteria of S. frugiperda larvae in four different geographical populations in Yunnan are rich,and there are differences in the diversity of intestinal bacteria in different geographical populations. But the dominant bacteria are Enterococcus,and Enterococcus bacteria play a decisive role in the intestinal bacterial community of S. frugiperda.