海南热带雨林土壤细菌组成与多样性分析

杜昊楠, 兰国玉, 吴志祥, 陈伟, 杨川

杜昊楠, 兰国玉, 吴志祥, 陈伟, 杨川. 2022: 海南热带雨林土壤细菌组成与多样性分析. 南方农业学报, 53(3): 840-849. DOI: 10.3969/j.issn.2095-1191.2022.03.026
引用本文: 杜昊楠, 兰国玉, 吴志祥, 陈伟, 杨川. 2022: 海南热带雨林土壤细菌组成与多样性分析. 南方农业学报, 53(3): 840-849. DOI: 10.3969/j.issn.2095-1191.2022.03.026
DU Hao-nan, LAN Guo-yu, WU Zhi-xiang, CHEN Wei, YANG Chuan. 2022: Study on soil bacterial composition and diversity of tropical rainforest in Hainan Island. Journal of Southern Agriculture, 53(3): 840-849. DOI: 10.3969/j.issn.2095-1191.2022.03.026
Citation: DU Hao-nan, LAN Guo-yu, WU Zhi-xiang, CHEN Wei, YANG Chuan. 2022: Study on soil bacterial composition and diversity of tropical rainforest in Hainan Island. Journal of Southern Agriculture, 53(3): 840-849. DOI: 10.3969/j.issn.2095-1191.2022.03.026

海南热带雨林土壤细菌组成与多样性分析

基金项目: 

海南省自然科学基金(高层次人才项目)(320RC733)

国家现代农业产业技术体系建设专项(CARS-33-ZP3)

海南省重点研发计划社会发展方向项目(ZDYF2019145)

国家自然科学基金项目(31770661)

详细信息
    作者简介:

    杜昊楠(1997-),https://orcid.org/0000-0003-1905-428X,研究方向为土壤微生物组成与多样性,E-mail:2932520020@qq.com

    通讯作者:

    兰国玉(1977-),https//orcid.org/0000-0003-4019-4252,博士,研究员,主要从事森林生态学研究工作,E-mail:langyrri@163.com

  • 中图分类号: S757

Study on soil bacterial composition and diversity of tropical rainforest in Hainan Island

Funds: 

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Hainan Provincial Key Research and Development Plan Social Development Direction Project(ZDYF2019145)

Hainan Natural Science Foundation(High-level Talent Program)(320RC733)

Industrial Technology System(CARS-33-ZP3)

  • 摘要: 【目的】明确海南热带雨林土壤细菌的组成与多样性、时空分布规律及对土壤理化性质的响应和功能结构,为海南地区热带雨林的管理和保护提供理论依据。【方法】对海南五大热带雨林吊罗山、五指山、鹦哥岭、霸王岭和尖峰岭的土壤进行采样,每个样地采集13份样本,分旱、雨两季,共130份样本;通过高通量测序技术,分析土壤样本微生物中的细菌组成与多样性随空间位置和季节的变化,应用PICRUSt2功能预测分析土壤细菌功能。【结果】OTU分类统计显示,旱季的细菌OTU数目为10958,雨季的细菌OTU数目为11533,两个季节间差异不显著(P>0.05)。在细菌门水平,热带雨林旱季和雨季土壤细菌的优势菌群为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)和疣微菌门(Verrucomicrobia);两个季节间的拟杆菌门(Bacteroidetes)、放线菌门和蓝藻菌门(Cyanobacteria)丰度差异极显著性((P<0.01,下同)。在细菌纲水平,热带雨林旱季和雨季土壤细菌的优势菌纲为放线菌纲(Actinobacteria)、酸杆菌纲(Acidobacteria)、α-变形菌纲(Alphaproteobacteria)、浮霉菌纲(Planctomycetacia)和斯巴托菌纲(Spartobacteria);两个季节间的浮霉菌纲、鞘脂杆菌纲(Sphingobacteriia)、纤线杆菌纲(Ktedonobacteria)和放线菌纲丰度差异极显著。冗余分析(RDA)表明pH和全钾对土壤中细菌的贡献度较高,解释率分别为5.03%和4.28%。ANOSIM和Adonis分析显示不同季节细菌多样性差异不明显,不同地点细菌多样性差异明显。KEGG通路显示吊罗山样本中的细菌功能丰度较其他4个样点少。【结论】海南热带雨林土壤细菌在不同地理位置(取样点)的多样性差异明显,季节变换对土壤细菌的组成影响不明显,pH和全钾是土壤中细菌旱、雨季多样性的重要环境因子。
    Abstract: 【Objective】To clarify the soil bacterial composition,diversity,spatio-temporal distribution,response to soil physical and chemical properties and functional structures in tropical rainforests of Hainan Island,so as to provide a theoretical basis for the management and protection of the tropical rainforests in Hainan.【Method】Soil samples were collected from Diaoluoshan,Wuzhishan,Yinggeling,Bawangling and Jianfengling tropical rainforest reserves in Hainan. Thirteen samples were collected from each reserve in both the dry and wet seasons,producing a total of 130 samples. The composition and diversity of bacteria in soil microorganisms were analyzed with a high-throughput sequencing technique and the function of soil bacteria was predicted and analyzed by PICRUSt2 software.【Result】Operational taxonomic unit (OTU)classification statistics show that the number of bacterial OTU in the dry season is 10958 and that in rainy season this increases to 11533. There was no significant difference between the two seasons(P>0.05). At the level of bacteria phylum,the dominant flora of soil bacteria in tropical rain forest in dry season and rainy season were Proteobacteria,Acidobacteria,Actinobacteria,Planctomycetes and Verrucomicrobia. The abundance of Bacteroidetes,Actinobacteria and Cyanobacteria in two seasons showed significant differences(P<0.01,the same below). At the level of bacterial class,the dominant classes in the dry and rainy seasons were Actinomycetia,Acidobacteriia,Alphaproteobacteria,Planctomycetia and Spartobacteria. There were significant differences among the classes Planctomyceteria,Sphingobacteriia,Ktedonobacteria and Actinomycetia. Redundancy analysis(RDA)showed that pH and total potassium(TK)affected the soil bacterial composition substantially,with explanation rates of 5.03% and 4.28% respectively. ANOSIM and Adonis analysis showed that there was no significant difference in bacterial diversity in the different seasons,but significant difference in bacterial diversity in different places. KEGG pathway showed that the bacterial functional abundance in the Diaoluoshan samples was less than that from the other four reserves.【Conclusion】The diversity of soil bacteria in Hainan tropical rainforests is significantly different in different geographical locations(sampling sites),whereas the seasonal change does not affect the composition of soil bacteria significantly. Soil pH and total potassium(TK)content have important effects on the soil bacterial diversity in the dry and rainy seasons.
  • 陈燕飞.2009.pH对微生物的影响[J].太原师范学院学报(自然科学版),8(3):121-124.[Chen Y F.2009.pH to uygur biology influence[J].Journal of Taiyuan Normal University (Natural Science Edition),8(3):121-124.]doi: 10.3969/j.issn.1672-2027.2009.03.032.
    程文文,罗珠珠,牛伊宁,蔡霞.2020.黄土高原雨养区苜蓿地土壤微生物功能多样性研究[J].河南农业科学,49(11):61-70.[Cheng W W,Luo Z Z,Niu Y N,Cai X.2020.Microbes functional diversity of alfalfa soil in the rainfed loess plateau[J].Journal of Henan Agricultural Sciences,49(11):61-70.]doi: 10.15933/j.cnki.1004-3268.2020.11.008.
    褚海燕,冯毛毛,柳旭,时玉,杨腾,高贵锋.2020.土壤微生物生物地理学:国内进展与国际前沿[J].土壤学报,57(3):515-529.[Chu H Y,Feng M M,Liu X,Shi Y,Yang T,Gao G F.2020.Soil microbial biogeography:Recent advances in China and research frontiers in the world[J].Acta Pedologica Sinica,57(3):515-529.]doi: 10.11766/trxb202001090010.
    黄萍,王楠,周紫羽,王婷,袁志良,常介田,叶永忠.2020.白云山落叶阔叶林土壤细菌群落结构及环境因子的相关性分析[J].河南农业大学学报,54(3):415-421.[Huang P,Wang N,Zhou Z Y,Wang T,Yuan Z L,Chang J T,Ye Y Z.2020.Soil bacterial community structure and correlation analysis of environmental factors in deciduous broad-leaved forest of Baiyun Mountain[J].Journal of Henan Agricultural University,54(3):415-421.]doi: 10.16445/j.cnki.1000-2340.2020.03.005.
    李君,兰国玉,李玉武.2016.海南儋州橡胶林与热带次生林土壤微生物区系研究[J].热带作物学报,37(3):433-438.[Li J,Lan G Y,Li Y W.2016.Comparison on soil micro flora in rubber forest and tropical secondary forest in Danzhou,Hainan Island[J].Chinese Journal of Tropical Crops,37(3):433-438.]doi: 10.3969/j.issn.1000-2561.2016.03.001.
    李永恒,李乾玺,吴君君,程晓莉.2020.秦岭落叶阔叶林不同空间尺度下土壤微生物特征[J].植物科学学报,38(3):335-346.[Li Y H,Li Q X,Wu J J,Cheng X L.2020.Soil microbial attributes at different spatial scales in deciduous broad-leaved forest in Qinling Mountains[J].Plant Science Journal,38(3):335-346.]doi: 10.11913/PSJ.2095-0837.2020.335.
    刘殿锋.2011.含钾岩石生物转化中的微生物多样性与作用机制研究[D].南京:南京师范大学.[Liu D F.2011.Microbial diversities during bioconversion of potassiumbearing rocks and its mechanisms[D].Nanjing:Nanjing Normal University.]
    刘明航,李盼畔,陈萍,文彬.2018.西双版纳热带雨林的土壤种子库与雨林保护[J].中国野生植物资源,37(5):56-60.[Liu M H,Li P P,Chen P,Wen B.2018.Xishuangbanna tropical rainforest soil seed bank and rainforest conservation[J].Chinese Wild Plant Resources,37(5):56-60.]doi: 10.3969/j.issn.1006-9690.2018.05.012.
    鲁如坤.2000.土壤农业化学分析方法[M].北京:中国农业科技出版社.[Lu R K.2000.Analytical methods of soil agrochemistry[M].Beijing:China Agricultural Science and Technology Press.]
    马萍,张玲琪,魏蓉城,王正显,江东福.1997.西双版纳橡胶、胡椒和咖啡根际土壤放线菌区系研究[J].云南大学学报(自然科学版),19(4):393-396.[Ma P,Zhang L Q,Wei R C,Wang Z X,Jiang D F.1997.Studies on the rhizosphere actinomycete microflora of rubber tree,pepper,coffee in Xishuang Banna[J].Journal of Yunnan University (Natural Sciences Edition),19(4):393-396.]
    彭玉娇,崔学宇,邵元元,杨艳丽,杜潇,贾书刚,刘书田,区燕丽.2021.不同树龄沙田柚果园土壤肥力、叶片养分和土壤细菌群落的特征[J].江苏农业学报,37(2):348-354.[Peng Y J,Cui X Y,Shao Y Y,Yang Y L,Du X,Jia SG,Liu S T,Ou Y L.2021.Characteristic of soil fertility,leaf mineral nutrients and bacterial community in Shatian pomelo orchards of different tree ages[J].Jiangsu Journal of Agricultural Sciences,37(2):348-354.]doi: 10.3969/j.issn.1000-4440.2021.02.010.
    全飞,兰国玉,李玉武,李君.2020.橡胶树林长期喷施硫磺对土壤细菌多样性的影响[J].西北林学院学报,35(2):131-136.[Quan F,Lan G Y,Li Y W,Li J.2020.Effects of long-term sulfur spraying on soil bacterial diversity in rubber plantations[J].Journal of Northwest Forestry University,35(2):131-136.]doi: 10.3969/j.issn.1001-7461.2020.02.19.
    谭雪莲,阚蕾,张璐,郑嘉仪.2019.城市森林土壤微生物群落结构的季节变化[J].生态学杂志,38(11):3306-3312.[Tan X L,Kan L,Zhang L,Zheng J Y.2019.Seasonal dynamics of soil microbial community structure in urban forest[J].Chinese Journal of Ecology,38(11):3306-3312.]doi: 10.13292/j.1000-4890.201911.017.
    王春香,田宝玉,吕睿瑞,林伟铃,徐燕,黄钦耿,黄建忠.2010.西双版纳地区热带雨林土壤酸杆菌(Acidobacteria)群体结构和多样性分析[J].微生物学通报,37(1):24-29.[Wang C X,Tian B Y,LüR R,Lin W L,Xu Y,Huang Q G,Huang J Z.2010.Distribution and diversity of Acidobacteria in tropical rain forest soil of Xishuangbanna[J].Microbiology China,37(1):24-29.]doi: 10.13344/j.microbiol.china.2010.01.024.
    王晓雯,洪振瀚,刘安瑞,罗立新.2016.基于荧光定量PCR和高通量测序技术的葡萄园土壤细菌群落结构多样性分析[J].酿酒科技,(11):28-33.[Wang X W,Hong Z H,Liu A R,Luo L X.2016.Analysis of bacterial community structure in vineyard soil by RT-PCR and high throughput sequencing[J].Liquor-Making Science&amp;Technology,(11):28-33.]doi: 10.13746/j.njkj.2016279.
    吴溪玭.2015.南亚热带五种人工林林下植物与土壤微生物群落变化及其环境解释[D].南宁:广西大学.[Wu X P.2015.Variation analysis and environmental interpretation of the understory and soil microbial communities of five different plantations in Southern Subtropical Area of China[D].Nanning:Guangxi University.]
    杨帆.2016.热带雨林转变为橡胶林对土壤微生物的影响研究[D].昆明:云南师范大学.[Yang F.2016.Research on microorganism changes under the transformation from tropical rain forests to rubber plantations[D].Kunming:Yunnan Normal University.]
    杨泽良,李萍芳,薛涛,向书琴,向国红.2020.玉米根际土壤微生物群落结构及多样性季节变化特征[J].西南农业学报,33(5):1001-1010.[Yang Z L,Li P F,Xue T,Xiang S Q,Xiang G H.2020.Seasonal variation characteristics of soil microbial community structure and diversity of corn in rhizosphere soil[J].Southwest China Journal of Agricultural Sciences,33(5):1001-1010.]doi: 10.16213/j.cnki.Scjas.2020.5.016.
    姚斌,韦秀文,刘惠文,周玲莉,尚鹤.2011.树木修复过程中微生物对酸化剂的响应[J].环境科学与技术,34(12):105-109.[Yao B,Wei X W,Liu H W,Zhou L L,Shang H.2011.Effects of soil pH regulators on soil microbial biomass in atrazine-contaminated soil by dendroremediation of poplar[J].Environmental Science&amp;Technologry,34(12):105-109.]doi: 10.3969/j.issn.1003-6504.2011.12.022.
    姚琦,尤青,李媛宏,张起畅,殷浩能,吴红萍,王锐萍.2017.海南东寨港红树林土壤微生物功能多样性[J].应用与环境生物学报,23(5):857-861.[Yao Q,You Q,Li Y H,Zhang Q C,Yin H N,Wu H P,Wang R P.2017.Functional diversity of soil microorganisms in the soil in Dongzhaigang mangrove wetlands in Hainan[J].Chinese Journal of Applied&amp;Environmental Biology,23(5):857-861.]doi: 10.3724/SP.J.1145.2016.11001.
    张亮.2013.微生物对土壤磷钾的活化作用[D].重庆:西南大学.[Zhang L.2013.Mobilization of phosphorus and potassium from soils by microorganisms[D].Chongqing:Southwest University.]
    朱华,周虹霞.2002.西双版纳热带雨林与海南热带雨林的比较研究[J].云南植物研究,24(1):1-13.[Zhu H,Zhou H X.2002.A comparative study on the tropical rain forests in Xishuangbanna and Hainan[J].Acta Botanica Yunnanica,24(1):1-13.]doi: 10.3969/j.issn.2095-0845.2002.01.001.

    Fierer N,Jackson R B.2006.The diversity and biogeography of soil bacterial communities[J].Proceedings of the National Academy of Sciences of the United States of America,103(3):626-621.doi: 10.1073/pnas.0507535103.

    Lan G Y,Li Y W,Lesueur D,Wu Z X,Xie G S.2018.Seasonal changes impact soil bacterial communities in a rubber plantation on Hainan Island,China[J].Science of the Total Environment,626(1):862-834.doi: 10.1016/j.scitotenv.2018.01.147.

    Lan G Y,Li Y W,Wu Z X,Xie G S.2017.Impact of tropical forest conversion on soil bacterial diversity in tropical region of China[J].European Journal of Soil Biology,83:91-97.doi: 10.1016/j.ejsobi.2017.10.007.

    Lan G Y,Wu Z X,Li Y W,Chen B Q.2020a.The drivers of soil bacterial communities in rubber plantation at local and geographic scales[J].Archives of Agronomy and Soil Science,66(3):358-369.doi: 10.1080/03650340.2019.1616286.

    Lan G Y,Wu Z X,Yang C,Sun R,Chen B Q,Zhang X.2020b.Tropical rainforest conversion into rubber plantations results in changes in soil fungal composition,but underling mechanisms of community assembly remain unchanged[J].Geoderma,375:114505.doi: 10.1016/j.geoderma.2020.114505.

    Lauber C L,Strickland M S,Bradford M A,Fierer N.2008.The influence of soil properties on the structure of bacterial and fungal communities across land-use types[J].Soil Biolgy Biochemistry,40(9):2407-2415.doi: 10.1016/j.soilbio.2008.05.021.

    Liu L M,Yang J,Yu X Q,Chen G J,Yu Z.2013.Patterns in the composition of microbial communities from a subtropical river:Effects of environmental,spatial and temporal factors[J].PLoS One,8(11):e81232.doi: 10.1371/journal.pone.0081232.

    Schjønning P,Thomsen I K,Moldrup P,Christensen B T.2003.Linking soil microbial activity to water-and airphase contents and diffusivities[J].Soil Science Society of America Journal,67:156-165.doi: 10.2136/sssaj2003.1560.

    Stres B,Danevcic T,Pal L,Fuka M M,Resman L,Leskovec S,Hacin J,Stopar D,Mahne I,Mulec M I.2008.Influence of temperature and soil water content on bacterial,archaeal and denitrifying microbial communities in drained fen grassland soil microcosms[J].FEMS Microbiology Ecology,66(1):110-122.doi: 10.1111/j.1574-6941.2008.00555.x.

    Xue P P,Carrillo Y,Pino V,Minasny B,McBratney A B.2018.Soil properties drive microbial community structure in a large scale transect in South Eastern Australia[J].Scientific Reports,8(1):11725.doi: 10.1038/s41598-018-30005-8.

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