4种中药复方对长江鲟幼鱼抗氧化和抗应激指标的影响

Effects of four Chinese herbal medicine compound on antio-xidation and anti-stress indexes of Acipenser dabryanus juveniles

  • 摘要: 【目的】探讨不同中药复方对长江鲟幼鱼抗氧化和抗应激指标的影响,筛选能有效提高长江鲟幼鱼免疫力及抗病力的中药复方,为中药在长江鲟养殖生产中的推广应用提供参考依据。【方法】将17味中药按照中药配伍理论设计4种中药复方,分别是复方I(鱼腥草:金银花:大黄:茯苓:甘草︰黄芪=3:3:1:2:1:2)、复方II(板蓝根:大黄:五加皮:党参=3:3:4:3)、复方III(金银花:杏仁:贯众:大青叶:山豆根:桔梗=3:2:2:2:3:2)、复方IV(黄芪:黄柏:甘草:山楂:五味子:大黄:党参=3:3:1:2:2:2:2),以不添加中药为对照组,按0.5 g/kg的添加量进行长江鲟幼鱼饲喂试验,并于第7和第14 d采集血样测定各项血清生理指标。【结果】与对照组相比,复方I组和复方II组可不同程度地提高长江鲟血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化物酶(POD)、碱性磷酸酶(AKP)活性及总抗氧化能力(T-AOC)、热休克蛋白70(HSP70)含量,降低丙二醛(MDA)含量,但仅部分指标差异达显著水平(P<0.05,下同);复方III组和复方IV组可明显提高长江鲟血清SOD、CAT、GSH-Px、POD、AKP活性及T-AOC,降低MDA和皮质醇(COR)含量,且大部分指标与对照组的差异达显著水平。相关分析结果表明,SOD活性与GSH-Px活性及T-AOC、POD活性与GSH-Px活性呈极显著正相关(P<0.01,下同),POD活性与SOD活性、CAT活性及T-AOC呈显著正相关,SOD活性与COR含量、T-AOC与COR含量呈极显著负相关,GSH-Px活性与COR含量、T-AOC与MDA含量呈显著负相关。【结论】在基础饲料中添加适宜的中药复方对长江鲟幼鱼抗氧化和抗应激能力有显著促进作用,尤其以复方III(金银花、杏仁、贯众、大青叶、山豆根、桔梗)和复方IV(黄芪、黄柏、甘草、山楂、五味子、大黄、党参)的效果最佳,可有效缓解长江鲟幼鱼机体面临的应激反应,达到保护效果。

     

    Abstract: 【Objective】To test the effects of four different Chinese herbal medicine compound on the anti-oxidation and anti-stress physiological indexes of Acipenser dabryanus juveniles,so as to provide reference for the promotion and application of traditional Chinese medicine in the breeding and production of A. dabryanus juveniles.【Method】Four medicin componnds were formulated from 17 sources used in traditional Chinese herbal medicines according to the theory of Chinese medicine compatibility. The formulations were:compound I(Houttuynia cordata Thunb:Lonicera japonica Thunb:Rheum palmatum L.:Poria:Glycyrrhiza uralensis Fisch:Astragalus mongholicus Bunge=3:3:1:2:1:2);compound II (Isatis tinctoria:R. palmatum L.:Acanthopanar gracilistμlus W. W. Smith:Codonopsis pilosula=3:3:4:3);compound III(L. japonica Thunb:Prunus armeniaca L.:Cyrtomium fortunei J. Sm:Clerodendrum cyrtophyllum Turcz:Radix sophorae Tonkinensis:Platycodon grandiflorus=3:2:2:2:3:2) and compound IV (A. mongholicus Bunge:Phellodendron:G. uralensis Fisch:Crataegus pinnatifida Bunge:Schisandra chinensis:R. palmatum L.:C. pilosula=3:3:1:2:2:2:2).The basic feed without any Chinese herbal medicine was used as the control group. The compound Chinese herbal medicines were fed at a dosage of 0.5 g/kg body weight during 14 days of the breeding period. Different physiological indexes of juvenile serum were detected on the 7th and 14th days.【Result】Compared with the control group,compounds I and II enhanced the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),peroxidase (POD),alkaline phosphatase(AKP),the total antioxidant capacity(T-AOC) and content of heat shock protein 70 (HSP70). The contents of malondialdehyde(MDA) and cortisal(COR) of the juvenile serum was reduced to varying degree,but only some differences were significant(P<0.05,the same below). Compounds III and IV clearly enhanced the activities of SOD,CAT,GSH-Px,POD and AKP,the contents of T-AOC and reduced the contents of MDA and COR. Most altered indexes were significant compared with the control. The results of correlation analysis showed that, the remarkably significant posi-tive correlation was found between SOD and GSH-Px,SOD and T-AOC,POD and GSH-Px(P <0.01,the same below). A significant positive correlation was also found between POD and SOD,CAT and T-AOC. Furthermore,both SOD and T-AOC showed a remarkably significant negative correlation with COR. Further significant and negative correlations were observed between GSH-Px and COR,T-AOC and MDA.【Conclusion】Adding suitable Chinese herbal medicine compounds into the basic diet can significantly promote the antioxidant and anti-stress capabilities in A. dabryanus juveniles. In particular, compound III(L. japonica Thunb,P. armeniaca L.,C. fortunei J.Sm,C. cyrtophyllum Turcz,R. sophorae Tonkinensis) and compound IV(A. mongholicus Bunge,Phellodendron,G. uralensis Fisch,C. pinnatifida Bunge,S. chinensis,R. palmatum L.,C. pilosula) have the best effect. These formulations can effectively relieve and protect A. dabryanus juveniles against stress.

     

/

返回文章
返回