不同浓度镧对茶树根系抗氧化酶活性及根细胞超微结构的影响

Effects of different concentrations of lanthanum on antioxidant enzyme activity and cell ultrastructure in the root of teaplant (Camellia sinensis)

  • 摘要: 【目的】探究不同浓度镧(La)对铁观音和水仙茶树根系抗氧化酶活性和根细胞超微结构的影响,为选育镧低吸收茶树品种提供数据参考。【方法】以茶树品种水仙和铁观音为试材,测定分析不同浓度La3+溶液(0.08、0.40和1.20 mmol/L)处理下根系的抗氧化酶活性和根细胞超微结构的变化以及镧在茶树根组织部位的定位分布。【结果】La3+浓度为1.20 mmol/L时,铁观音根系的超氧化物歧化酶(SOD)活性比水仙高73.9%,过氧化物酶(POD)活性及过氧化氢酶(CAT)活性与水仙根系相比无显著差异(P>0.05)。扫描电镜观察发现,高浓度La3+处理下根系细胞排列疏松,无规则,蜡质增多;品种间相比,水仙较铁观音根细胞蜡质覆盖量多,受害程度较重。透射电镜观察发现,高浓度La3+处理下2个茶树品种根系细胞排列散乱,细胞壁膨大,细胞壁、细胞质膜上分布较多黑色沉积物,进一步结合能谱分析,发现黑色沉积物中含有镧元素,且主要分布于铁观音根细胞中的原生质体,而在水仙根细胞的细胞壁及原生质体中均有积累。【结论】镧处理后铁观音根系较水仙抗氧化酶活性增高,根细胞超微结构受害较轻,综合表现受镧影响较小,可作为选育镧低吸收茶树品种材料加以深入研究和开发。

     

    Abstract: 【Objective】To explore the effects of different concentrations of lanthanum on the antioxidant enzyme activity and the ultrastructure of root cells of Tieguanyin and Shuixian,soasto provide data reference for breeding tea varieties with low lanthanum absorption.【Method】Taking Shuixian and Tieguanyin tea as material to examine the antioxidant enzyme activity and root cell ultrastructure and the localization and distribution of lanthanum in response to treatment with 0.08,0.40,or 1.20 mmol/L La3+.【Result】When the lanthanum concentration is 1.20 mmol/L,the superoxide dismutase (SOD) activity of Tieguanyin roots is 73.9% higher than that of Shuixian tea,and the peroxidase (POD) activity and catalase(CAT) activity are significantly lower than that of Shuixian tea roots. Scanning electron microscopy observation results showed thatunder the treatment of high concentration,the cells were arranged loosely,irregularly,and have morewax. Compared with different varieties,Shuixian tea had more waxy coverage,more severe damage,and weaker tolerancethan Tieguanyin root cells did. Transmission electron microscopy observation results showed that the root cells of the two tea plant varieties under high concentration of lanthanum scattered irregularly,the cell walls were enlarged,and more black sediments could be found on the cell walls. According to energy spectrum analysis,lanthanum was found in the black sediment,which was mainly distributed in the protoplast of Tieguanyin root cells,and accumulated in the cell walls and protoplast of Shuixian tea root cells.【Conclusion】After lanthanum treatment,the antioxidant enzyme activity of Tieguanyin root was higher than that of Shuxian tea,and the Tieguanyin tea root cell ultrastructure was damaged less,and Tieguanyin tea was less affected by lanthanum. Thus,Tieguanyin teacan be used as a material for breeding lanthanum low absorption tea varieties for in-depth research and development.

     

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