Genetic diversity of yam germplasm revealed by chloroplast DNA sequences atpI-rsp2 and psbC-trnS
-
摘要: 【目的】基于叶绿体DNA(cpDNA)序列atpI-rsp2和psbC-trnS分析山药种质资源的遗传多样性,为山药种质资源鉴定、创新利用及新品种选育提供理论依据。【方法】以来自14个省(区)的64份山药种质为材料,对其atpI-rsp2和psbC-trnS序列进行多态性扩增并测序,经拼接、比对后,利用MEGA 7.0计算种质间的遗传距离并构建系统发育进化树,并利用DNAsp 5.0分析核苷酸多态性,采用NET Framework 4.6.1绘制单倍型间的中介邻接网络结构。【结果】atpIrsp2和psbC-trnS序列合并序列长度为1938 bp,共有117个插入/缺失位点(IS)和14个变异位点(Vs),转换率(Si) 为49.1%,总体转换/颠换偏倚率(R)为0.928,共产生30种单倍型,其中有21种为独享单倍型,9种为共享单倍型,单倍型多样性(Hd)和核苷酸多样性(π)分别为0.9206和0.00139。atpI-rsp2序列和psbC-trnS序列及合并序列的Tajima’s D、Fu and Li’s D*和F*均为负值,其差异均未达显著水平(P>0.10),说明这2个序列在进化上符合中性进化模式。64份山药种质的遗传距离为0~0.003865,平均遗传距离均为0.001400。中介邻接网络结构分析结果显示,30种单倍型可分为3类,其中,H5为较原始单倍型。【结论】64份山药种质资源的遗传距离较近,遗传背景相似,可能是由于长期地区间引种导致,与地理距离不完全相关。atpI-rsp2和psbC-trnS序列可用于山药物种鉴定和系统进化分析等研究领域。Abstract: 【Objective】The genetic diversity of 64 yam germplasm was analyzed based on the chloroplast DNA(cpDNA)sequences atpI-rsp2 and psbC-trnS to provide a theoretical basis for the selection,innovative utilization and breeding of yam germplasm resources.【Method】The atpI-rsp2 and psbC-trnS sequences of 64 yam germplasm collected from 14 provinces(regions)were amplified and sequenced for polymorphism. After mosaic and comparison,the genetic distance between germplasms was calculated by MEGA 7.0 and a phylogenetic tree was constructed. The nucleotide polymorphisms were analyzed by DNAsp 5.0 and NET Framework 4.6.1 was used to draw the intermediate adjacency network structure between haplotypes.【Result】The combined sequence length of atpI-rsp2 and psbC-trnS was 1938 bp,with con-tained 117 insert/missing sites(IS)and 14 variable sites(Vs). The conversion frequency(Si) was 49.1%,and the overall transition/transversion bias(R)value was 0.928,resulting in 30 haplotypes. Of these,21 haplotypes were exclusive and 9 haplotypes were shared,corresponding to a haplotype diversity index value(Hd)of 0.92063 and nucleotide diversity value (π)of 0.00139. The Tajima’s D values,Fu and Li’s D * and F * of the atpI-rsp2 sequence and the psbC-trnS sequence and the merged sequences were all negative,and the difference was not significant(P>0.10),suggesting the sequences in yams fitted the neutral evolutionary model. The genetic distance of 64 yam germplasm ranged from 0 to 0.003865 with an average of 0.001400. The results of the structure analysis of the intermediate adjacency network showed that the 30 haplotypes could be divided into three types,H5 was the primitive haplotype.【Conclusion】The 64 yam germplasm resources have close genetic distance and similar genetic background,which may be caused by long-term interregional introduction. It is not entirely correlated with geographical distance. atpI-rsp2 and psbC-trnS sequences can be used for species identification and the phylogenetic analysis of yam.
-
Keywords:
- yam /
- chloroplast DNA(cpDNA) /
- atpI-rsp2 /
- psbC-trnS /
- genetic diversity /
- genetic differentiation
-
-
程月琴,李萍萍,王梦云,王昱程,王红卫. 2019.基于叶绿体DNA变异的怀山药鉴定体系构建[J].河南农业大学学报,53(5):759-765.[Cheng Y Q,Li P P,Wang M Y, Wang Y C,Wang H W. 2019. Construction of the system for the identification of Huai-yam (Dioscorea Opposita) based on chloroplast DNA variation[J]. Journal of Henan Agricultural University,53(5):759-765.]doi:10.16445/j.cnki.1000-2340.2019 0919.001. 付春霖,戴小康,李小燕,刘星. 2017.黑藻cpDNA分子标记的筛选及其遗传多样性的初步研究[J].植物科学学报,35(1):87-92.[Fu C L,Dai X K,Li X Y,Liu X. 2017. Screening cpDNA markers and preliminary study on the genetic diversity of Hydrilla verticillata from China[J]. Plant Science Journal,35(1):87-92.]doi: 10.11913/PSJ.2095-0837.2017.10087. 高丽杨,夏惠,谢玥,沈妍秋,王秀,梁东. 2017.基于cpDNA序列的猕猴桃种质资源多态性分析[J].分子植物育种,15(9):3751-3758.[Gao L Y,Xia H,Xie Y,Shen Y Q, Wang X,Liang D. 2017. Molecular polymorphic analyses for the germplasms of Actinidia based on cpDNA[J]. Molecular Plant Breeding,15(9):3751-3758.] doi:10. 13271/j.mpb.015.003751. 高源,王大江,王昆,丛佩华,张彩霞,李连文,朴继成. 2020.基于叶绿体DNA分析的楸子种质遗传多样性研究[J].园艺学报,47(5):853-863.[Gao Y,Wang D J,Wang K, Cong P H,Zhang C X,Li L W,Piao J C. 2020. Genetic diversity of Malus prunifolia germplasms based on chloroplast DNA analysis[J]. Acta Horticulturae Sinica,47(5):853-863.]doi: 10.16420/j.issn.0513-353x.2019-0609 雷伏贵. 2011.山药种质资源遗传多样性的ISSR分析[D].福州:福建农林大学.[Lei F G. 2011. Analysis of genetic diversity of yam of germplasm rescources[D]. Fuzhou:Fujian Agriculture and Forestry University.] 刘振,成杨,杨培迪,赵洋,宁静,杨阳. 2020.基于nSSR和cpDNA序列的城步峒茶群体遗传多样性和结构研究[J].茶叶科学,40(2):250-258.[Liu Z,Cheng Y,Yang P D,Zhao Y,Ning J,Yang Y. 2020. Genetic diversity and structure of Chengbudong tea population revealed by nSSR and cpDNA markers[J]. Journal of Tea Science,40(2):250-258.]doi:10.13305/j.cnki.jts. 2020.02.011. 马双姣,周建国,李滢,陈新连,吴明丽,孙伟,李永华,宋经元,姚辉. 2018.薯蓣和叉蕊薯蓣叶绿体基因组及特异性DNA条形码鉴定序列筛选研究[J].中国科学:生命科学,48:571-582.[Ma S J,Zhou J G,Li Y,Chen X L, Wu M L,Sun W,Li Y H,Song J Y,Yao H. 2018. Complete chloroplast genomes of Dioscorea opposite and D.collettii and screening specific DNA barcodes[J]. Scientia Sinica Vitae,48:571-582.]doi: 10.1360/N052017-00160. 齐丹,常耀军,曹玉芬,胡红菊,田路明,董星光,张莹,霍宏亮,徐家玉,张小双,刘超. 2018.基于叶绿体DNA信息的南方梨属种质的遗传多样性和演化分析[J].园艺学报, 45(12):2308-2320.[Qi D,Chang Y J,Cao Y F,Hu H J, Tian L M,Dong X G,Zhang Y,Huo H L,Xu J Y,Zhang X S,Liu C. 2018. Genetic diversity and phylogenetics of pear (Pyrus L.) germplasm resources from South China revealed by chloroplast DNA[J]. Acta Horticulturae Sinica,45(12):2308-2320.]doi:10.16420/j.issn.0513-353x. 2018-0176. 沈奇,潘月云,臧春鑫,赵志平,关潇,张银东. 2019.基于叶绿体DNA单倍型的蒙古沙冬青遗传多样性格局探究[J].分子植物育种,17(4):1378-1384.[Shen Q,Pan Y Y, Zang C X,Zhao Z P,Guan X,Zhang Y D. 2019. Study on distribution pattern of genetic diversity of Ammopiptanthus mongolicus based on the haplotype of chloroplast DNA[J]. Molecular Plant Breeding,17(4):1378-1384.]doi: 10.13271/j.mpb.017.001378. 吴志刚. 2012.山药种质资源遗传亲缘关系与药材质量研究[D].广州:广州中医药大学.[Wu Z G. 2012. Study on genetic relationship and crude drugs quality of yam germplasm resources[D]. Guangzhou:Guangzhou University of Chinese Medicine.] 张安世,邢智峰,刘永英,张为民. 2009.苔藓植物DNA不同提取方法的比较分析[J].河南科学,27(5):559-562.[Zhang A S,Xing Z F,Liu Y Y,Zhang W M. 2009.Comparison and analysis on the different methods of DNA extraction methods from Bryophytes[J]. Henan Science, 27(5):559-562.]doi:10.13537/j. issn. 1004-3918.2009. 05.008. 张宏意,袁林林,饶秋红,李威,严寒静. 2018.不同产地何首乌叶绿体psbA-trnH基因序列分析[J].中草药,49(5):1146-1149.[Zhang H Y,Yuan L L,Rao Q H,Li W,Yan H J. 2018. Sequence analysis of psbA-trnH gene in chloroplast of Polygonum multiflorum from different produ-cing areas[J]. Chinese Traditional and Herbal Drugs,49(5):1146-1149.] doi:10.7501/j. issn. 0253-2670.2018. 05.024. 张蓉,殷亚方,徐魁梧,叶克林. 2014.基于DNA的木材树种识别研究进展[J].南京林业大学学报(自然科学版),38(1):151-156.[Zhang R,Yin Y F,Xu K W,Ye K L. 2014. Progress of wood identification based on DNA methods[J]. Journal of Nanjing Forestry University (Natural Sciences Edition),38(1):151-156.]doi: 10.3969/j.issn.1000-2006.2014.01.027. 章秋平,魏潇,刘威生,董文轩,刘宁,张玉萍,徐铭,刘硕,张玉君,马小雪. 2017.基于叶绿体DNA序列trnL-F分析李亚属植物的系统发育关系[J].果树学报,34(10):1249-1257.[Zhang Q P,Wei X,Liu W S,Dong W X,Liu N, Zhang Y P,Xu M,Liu S,Zhang Y J,Ma X X. 2017. Phylogenetic relationship in the plants of subgenus Prunophora (Rosaceae) inferred from the chloroplast DNA region, trnL-F[J]. Journal of Fruit Science,34(10):1249-1257.]doi: 10.13925/j.cnki.gsxb.20170098. 朱元娣,曹敏格,许正,王昆,张文. 2014.基于ITS和matK序列探讨新疆野苹果与中国苹果的系统演化关系[J].园艺学报,41(2):227-239.[Zhu Y D,Cao M G,Xu Z, Wang K,Zhang W. 2014. Phylogenetic relationship between Xinjiang wild apple (Malus sieversii Roem.) and Chinese apple (Malus×domestica subsp. chinesnsis) based on ITS and matK sequences[J]. Acta Horticulturae Sinica,41(2):227-239.]doi:10.16420/j.issn.0513-353x.2014. 02.027. Kumar S,Stecher G,Tamura K. 2016. MEGA7:Molecular evolutionary genetics analysis version 7.0 for Bigger Datasets[J]. Molecular Biology and Evolution,33(7):1870-1874. doi: 10.1093/molbev/msw054.
Polzin T,Daneshmand S V. 2003. On Steiner trees and minimum spanning trees in hypergraphs[J]. Operations Research Letters,31(1):12-20. doi: 10.1016/S0167-6377(02)00185-2.
Posada D,Crandall K A. 2001. Intraspecific gene genealogies:trees grafting into networks[J]. Trends in Ecology and Evolution,16:37-45. doi: 10.1016/S0169-5347(00)02026-7.
-
期刊类型引用(3)
1. 朱绍杰,王宇飞,于丹,都晓伟. 分子生物学技术在薯蓣属植物中的应用研究进展. 中草药. 2024(20): 7192-7202 . 百度学术
2. 张志飞,赵中振,王馨,尹光耀,陈颖,满金辉,石玥,黄钰莹,刘珊瑚,刘子齐,王晓晖,魏胜利. 刺五加特异性DNA条形码筛选、种质资源鉴定及遗传多样性分析. 中国中药杂志. 2023(05): 1229-1237 . 百度学术
3. 胡赛文,马良,丁怡宁,毕光耀,李贺敏,黄岩,夏至. 路边青叶绿体基因组特征与系统进化分析. 河南农业大学学报. 2022(03): 438-446 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 38
- HTML全文浏览量: 2
- PDF下载量: 4
- 被引次数: 4