Abstract: 【Objective】The effects of Ophiopogon japonicus intercropping with tea plantation in springon soil nutrient，enzyme activity and microbial community structure were analyzed to study the interaction mechanism between soil environment and microorganism under different culture mode conditions，and provide theoretical basis for intercropping practice of tea plantation.【Method】The tea plantation located at mountain terrace was used as the research objective to analyze the nutrient content，enzyme activity，bacterial community structure and diversity in soilunder tea monoculture，teaO. japonicusi intercropping and tea plantation soil（CK）by using 16S rDNA high-throughput sequencing technology.【Result】Compared to monoculture，O. japonicusi intercropping significantly increased soil water content，organic matter， available nitrogen（N），available phosphorus（P），available potassium（K），urease，protease，cellulase and acid phosphatase（P<0.05，the same below），while pH was not evidently changed. The diversity and richness of soil bacterial community in tea plantation were not greatly influenced by intercropping as compared with that of monoculture and CK，whereas the community structure was significantly changed. The relative abundances of Proteobacteria，Actinobacteria and Gemmatimonadetes were significantly increased in the intercropping treatment，while Chloroflexi was significantly decreased. The correlation analysis showed that Proteobacteria and Actinomycetes were significantly positively correlated with the soil available nutrients，soil organic matter and enzymes；Gemmatimonadetes were significantly positively correlated with available N and available P；Chloroflexi had negative correlation with soil organic matter. LEfSe analysis results showed that Rhizobiales，Xanthomonadales，Rhodanobacteraceae，Chujaibacter and unidentified Burkholderiaceae from Proteobacteria，and unidentified Actinobacteria from Actinobacteria were the biomark microorganisms in soil of intercropping tea plantation，and their abundance were significantly higher than that of CK and monoculture treatment.【Conclusion】 Intercropping O. japonicusi in tea plantation can alter the community structure of soil bacteria and enrich more beneficial bacteria that involve in nutrient cycling and antagonistic pathogens，which is conducive to improve soil nutrient supply capacity and tea disease resistance.