Analysis of vitamin E content and heterosis in sweet corn（Zea mays L.）
-
摘要: 【目的】分析不同甜玉米杂交种的杂种优势，筛选高维生素E（VE）组合及优异亲本，为高效创制高VE甜玉米品种提供材料基础和理论依据。【方法】采用正相高效液相色谱法（NP-HPLC），对11份不同来源且表型差异明显的甜玉米自交系及其11个杂交种进行VE组分含量测定，并分析其杂种优势，筛选高VE甜玉米材料。【结果】甜玉米自交系及杂交种中的VE组分间含量差异均达极显著水平（P<0.01，下同）。自交系26F白、金皇和杂交种粤甜41号、粤甜29号、粤甜35号的总生育酚（TTF）、总生育三烯酚（TT3）和总VE（TVE）含量较高。杂交种VE组分含量与双亲均值和高值亲本相关，其中α-生育酚（αT）和α-生育三烯酚（αT3）含量与高值亲本和双亲均值呈显著（P<0.05）或极显著正相关。11个杂交种VE组分含量的杂种优势主要表现为负向中亲优势，TVE含量的中亲优势变幅为-39.07%~56.97%，超亲优势变幅为-52.44~49.28%。11个杂交种中有4个表现出超亲优势，其中TY6和农宝2021白组配的杂交种粤甜41号、粤甜38号和粤甜35号具有较强的超亲优势，且TVE含量较高。【结论】不同基因型甜玉米间VE组分含量存在明显差异，VE含量的杂种优势主要表现为负向中亲优势；高VE亲本间杂交并不一定能够获得具有强杂种优势的杂交种。要提高甜玉米杂交种VE含量，选择高VE含量尤其是高αT和αT3含量的材料进行组配或许可加速品种培育进程。Abstract: 【Objective】The heterosis of hybrids derived from different sweet corn inbred lines were analyzed to excavate the combination and parents with high vitamin E（VE）content，so as to provide materials and theoretical basis for efficiently breeding high VE content sweet corn varieties.【Method】Normal phase high performance liquid chromatography （NP-HPLC）was used to determine the content of VE components in 11 sweet corn inbred lines with different origins and obvious phenotypic differences and their 11 hybrid combinations. The heterosis of VE components was analyzed to screen the high content sweet corn materials.【Result】The contents of VE components in inbred lines and hybrids indicated extremely significance level（P<0.01，the same below）. The contents of total tocophenol（TTF），total tocophenol（TT3） and total VE（TVE）in inbred lines 26F Bai，Jinhuang and hybrids Yuetan 41，Yuetan 29 and Yuetan 35 were high. The contents of all VE components in hybrids were correlated with the mean values of parents and high-value parents；of which the contents of α-tocopherol（αT）and α-tocoptrienol（αT3）were significantly（P<0.05）or extremely positively correlated with the mean values of parents and high-value parents. In terms of heterosis analysis，the heterosis of VE content in 11 hybrids mainly showed negative heterosis；the TVE content mid-parentheterosisranged from - 39.07% to 56.97%，and the over-parent heterosis ranged from -52.44 to 49.28%. Four hybrids showed super-parent heterosis，and the hybrids Yuetian 41，Yuetian 38 and Yuetian 35 which derived from inbred lines TY6 and Nongbao 2021 Bai presented stronger heterosis and higher TVE content.【Conclusion】There are differences in VE content among different genotypes of sweet corn. The heterosis of VE content is mainly negative mid-parent heterosis. The parents with high VE contents may not obtain hybrid with strong heterosis. For improving the VE content in sweet corn，the materials with high VE content， especially those with high αT or αT3 contents，can be selected as parents to accelerate the breeding process.
-
Keywords:
- sweet corn /
- inbred line /
- vitamin E /
- heterosis
-
-
程昕昕,刘正. 2012. shsu双隐性甜玉米F2籽粒营养品质杂种优势分析[J]. 广西植物,32(3):382-385.[Cheng X X, Liu Z. 2012. Heterosis analysis of F2 kernels nutrition in shsu double recessive sweet corn[J]. Guihaia,32(3):382-385.]doi: 10.3969/j.issn.1000-3142.2012.03.020. 冯发强,王国华,王青峰,杨瑞春,李小琴. 2014. 甜玉米乳熟期籽粒维生素A源和维生素E组分的变异[J]. 作物学报, 40(7):1227-1234.[Feng F Q,Wang G H,Wang Q F, Yang R C,Li X Q. 2014. Variation of provitamin A and vitamin E components at milk stage of kernel development in sweet corn[J]. Acta Agronomica Sinica,40(7):1227-1234.]doi: 10.3724/SP.J.1006.2014.01227. 郭彦,杨洪双,张文会,蒋基建. 2005. 黑糯玉米主要品质性状的遗传分析[J]. 玉米科学,13(4):44-45.[Guo Y,Yang H S,Zhang W H,Jiang J J. 2005. Genetic analysis of major quality characters of black glutinous maize[J]. Journal of Maize Sciences,13(4):44-45.]doi: 10.3969/j.issn.1005-0906.2005.04.012. 韩亚丽,林春晶,赵丽梅,张春宝. 2020. 作物杂种优势预测研究进展[J]. 东北农业科学,45(3):30-34.[Han Y L,Lin C J,Zhao L M,Zhang C B. 2020. Research progress in prediction of crop heterosis[J]. Journal of Northeast Agricultural Sciences,45(3):30-34.]doi:10.16423/j. cnki. 1003-8701.2020.03.007. 郝小琴,吴子恺. 2006. 鲜食甜糯玉米主要营养品质的遗传规律研究[J]. 安徽农业科学,34(21):5457-5460.[Hao X Q,Wu Z K. 2006. Study on the genetic law of main nutritional quality of the sweet- waxy maize as fresh food[J]. Journal of Anhui Agricultural Sciences,34(21):5457-5460.]doi: 10.3969/j.issn.0517-6611.2006.21.004. 何松. 2017. Sh_2甜玉米杂交种主要性状杂种优势分析[D]. 滁州:安徽科技学院.[He S. 2017. Heterosis analysis of main characters of Sh_2 sweet corn hybrid[D]. Chuzhou:Anhui Science and Technology University.] 兰进好. 2005. 玉米强优势组合重要性状杂种优势遗传基础研究[D]. 沈阳:沈阳农业大学.[Lan J H. 2005. Study on the genetic basis of heterosis of important characters in strong heterosis combination of maize[D]. Shenyang:Shenyang Agricultural University.] 李晶晶,张文洋,王利锋,王浩,李会勇. 2021. 我国主推玉米品种亲本的遗传结构解析[J]. 河南农业科学,50(1):27-34.[Li J J,Zhang W Y,Wang L F,Wang H,Li H Y. 2021. Genetic structure analysis of parental inbred lines of main maize hybrid varieties in China[J]. Journal of Henan Agricultural Sciences,50(1):27-34.]doi:10.15933/j.cnki. 1004-3268.2021.01.004. 李坤,李高科,肖颖妮,于永涛,李余良,谢利华,朱文广,胡建广. 2020. 甜玉米品质遗传改良研究进[J]. 广东农业科学,47(11):70-77.[Li K,Li G K,Xiao Y N,Yu Y T,Li Y L,Xie L H,Zhu W G,Hu J G. 2020. Research progresses in genetic improvement of sweet corn quality[J]. Guangdong Agricultural Sciences,47(11):70-77.]doi: 10.16768/j.issn.1004-874X.2020.11.008. 梁耀星. 2010. 甜玉米农艺、品质性状相关及配合力分析[D]. 广州:华南农业大学.[Liang Y X. 2010. Correlation and combining ability analysis of agronomic and quality characters of sweet corn[D]. Guangzhou:South China Agricultural University.] 刘增兵,姜景彬,杨欢欢,姜秀明,李景富. 2019. 植物杂种优势的研究进展[J]. 分子植物育种,17(12):331-338.[Liu Z B,Jiang J B,Yang H H,Jiang X M,Li J F. 2019. Research advance of plant heterosis[J]. Molecular Plant Breeding, 17(12):331-338.]doi: 10.13271/j.mpb.017.004127. 王磊,张春义. 2019. 营养型农业的发展背景及进展[J]. 生物产业技术,(6):59-63[Wang L,Zhang C Y. 2019. Development background and progress of nutrition-orientated agriculture[J]. Biotechnology & Business,(6):59-63.]doi: 10.3969/j.issn.1674-0319.2019.06.006. 王晓明,谢振文,曾慕衡,乐素菊. 2005. 超甜玉米果穗形态和品质性状的杂种优势及遗传分析[J]. 中国农业科学,38(9):1931-1936.[Wang X M,Xie Z W,Zeng M H,Le S J. 2005. Heterosis and inheritance analysis of ear shape and quality characters in super sweet corn[J]. Scientia Agricultura Sinica,38(9):1931-1936.]doi: 10.3321/j.issn:0578-1752.2005.09.033. 中国营养学会. 2016. 中国居民膳食指南[M]. 第4版. 北京:人民卫生出版社.[Chinese Nutrition Society. 2016. Dietary guidelines for Chinese residents[M]. The 4th Edition.Beijing:People's Medical Publishing House.] Baseggio M,Murray M,Magallanes-Lundback M,Kaczmar N,Chamness J,Buckler E S,Smith M E,DellaPenna D, Tracy W F,Gore M A. 2019. Genome-wide association and genomic prediction models of tocochromanols in fresh sweet corn kernels[J]. The Plant Genome,12:1-17.doi: 10.3835/plantgenome2018.06.0038.
Feng F Q,Deng F,Zhou P,Yan J B,Wang Q F,Yang R C,Li X Q. 2013. QTL mapping for the tocopherols at milk stage of kernel development in sweet corn[J]. Euphytica, 193(3):409-417. doi: 10.1007/s10681-013-0948-5.
Hong Y P,Tan W S D,Liao W,Wong W S F. 2016. Vitamin E therapy beyond cancer:Tocopherol versus tocotrienol[J]. Pharmacology & Therapeutics,162:152-169. doi: 10.1016/j.pharmthera.2015.12.003.
Jiang Q,Christen S,Shigenaga M K,Ames B N. 2001. gamma-tocopherol,the major form of vitamin E in the US diet, deserves more attention[J]. The American Journal of Clinical Nutrition,74(6):714-722. doi: 10.1093/ajcn/74.6.714.
Jiang Q. 2017. Natural forms of vitamin E as effective agents for cancer prevention and therapy[J]. Advances in Nutrition,8(6):850-867. doi: 10.3945/an.117.016329.
Lee G Y,Han S N. 2018. The role of vitamin E in immunity[J]. Nutrients,10(11):1614-1632. doi: 10.3390/nu10111614.
Miyazawa T,Burdeos G C,Itaya M,Nakagawa K,Miyazawa T. 2019. Vitamin E:Regulatory redox interactions[J]. IUBMB Life,71(4):430-441. doi: 10.1002/iub.2008.
Sen C K,Khanna S,Roy S. 2006. Tocotrienols:Vitamin E beyond tocopherols[J]. Life Sciences,78(18):2088-98.doi: 10.1016/j.lfs.2005.12.001.
Waniek S,Giuseppe R D,Esatbeyoglu T,Plachta-Danielzik S, Lieb W. 2017. Vitamin E(α- and γ-Tocopherol)levels in the community:Distribution,clinical and biochemical correlates,and association with dietary patterns[J]. Nutrients, 10(1):3. doi: 10.3390/nu10010003.
Xiao Y N,Yu Y T,Li G K,Xie L H,Guo X B,Li J S,Li Y S,Hu J G. 2020. Genome-wide association study of vitamin E in sweet corn kernels[J]. The Crop Journal,8(2):341-350. doi: 10.1016/j.cj.2019.08.002.
Xie L H,Yu Y T,Mao J H,Liu H Y,Hu J G,Li T,Guo X B, Liu R H. 2017. Evaluation of biosynthesis,accumulation and antioxidant activityof vitamin E in sweet corn(Zea mays L.)during kernel development[J]. International Journal of Molecular Sciences,18(12):2780-2790. doi:10. 3390/ijms18122780.
-
期刊类型引用(2)
1. 刘晓刚，杨秀清，解甜甜. 平欧21号大果榛在山西栽培的生长表现及果实经济性状分析. 果树资源学报. 2022(05): 90-94 . 
百度学术
2. 解甜甜，冯泽辉，王晨林，任宇辰，王雅茹，杨秀清. 4份平欧榛种质生长动态及果实经济性状比较. 经济林研究. 2022(04): 29-41 . 百度学术
其他类型引用(2)
计量
- 文章访问数: 43
- HTML全文浏览量: 3
- PDF下载量: 4
- 被引次数: 4
下载: