Abstract: 【Objective】 The aim of this study was to evaluate the predation potential of Sphedanolestes Impressicollis Stal. against the nymphs of Myzus persicae(Sulzer) in tobacco fields, and provide a scientific theoretical basis for ecological control of aphids in tobacco field.【Method】 Under laboratory conditions, a hungry S. impressicollis 4^th instar nymph (for 24 h) and 1^st-4^th instar aphid nymphs were set up with different gradients in Petri dishes and the predation of S. impressicollis was observed to determine the functional response and predatory effect of S. impressicollis on aphid nymphs. The biological statistical analysis was performed using GraphPad Prism 8.0, DPS and other softwares. S. impressicollis and tobacco aphid with different densities under different temperature gradient and spatial pattern conditions By controlling a single variable method and constructing a linear regression equation, the influence of intraspecific interference on predation rate and density of the 4^th S. impressicollis instar nymph as well as temperature and spatial pattern on the predation was verified to evaluate the predation functional response model of the 4^th instar nymph of S. impressicollis to M. persicae.【Result】 The predatory functional responses of the 4^th instar nymphs of S. impressicollis to nymphs of M. persicae were consistent with Holling-II model, and the effects of its intraspecific disturbance and density on nymphs of M. persicae were consistent with the Hassell model and Watt model, respectively. In the predation of the 4^th instar nymphs of S. impressicollis on the 1^st-4^th instar nymphs of aphids, the 4^th instar nymphs of S. impressicollis were partial to the 3^rd and 4^th instar aphid nymphs, and had the largest predatory number on the 4^th instar aphid nymphs(the theoretical maximum of daily predation was 55.7), followed by the 3^rd instar aphid nymphs(the theoretical maximum of daily predation was 55.3). Under the conditions of specific space size and aphid number, there was disturbed among the 4^th instar nymphs of S. impressicollis, and the disturbance coefficient was 1.31. Moreover, with the increase of the density of S. impressicollis, the disturbance increased, and the average prey quantity decreased. At the same time, under the same aphid density but different spaces, with the increase of space, the predation amount of S. impressicollis decreased. Under 16-32℃, with the increase of temperature, the predation amount of the 4^th instar nymphs of S. impressicollis on the aphid nymphs gradually increased. The predation amount was the maximum under 32℃(32 aphids).【Conclusion】 S. impressicollis has the potential to control M. persicae in a tobacco field.