努比亚山羊KISS1基因多态性与繁殖性状的关联分析

Association analysis on KISS1 gene polymorphism with reproductive traits in Nubian goats

  • 摘要: 【目的】明确转移抑制素基因(KISS1)多态性与努比亚山羊繁殖性状(产羔数、初生重和断奶重)的关联,为进一步展开山羊分子标记辅助选择育种提供参考依据。【方法】利用DNA混合池结合Sanger测序筛选出努比亚山羊KISS1基因SNP位点,再通过MTA-Seq测序对355只努比亚山羊群体进行SNP位点的基因分型,并采用SAS 9.2的一般线性模型(GLM)对KISS1基因SNP位点与努比亚山羊的产羔表型性状进行关联分析。【结果】在努比亚山羊KISS1基因内含子1发现2个SNP位点(g.1341600A>G和g.1341674C>G)。其中,g.1341600A>G位点处于中度多态(0.25PHWE>0.05);而g.1341674C>G位点处于低度多态(PIC<0.25),已偏离Hardy-Weinberg平衡状态(PHWE<0.05)。g.1341600A>G位点GG和AG基因型个体的第一胎、第二胎及三胎联合产羔数均高于AA基因型个体,且在第一胎达显著水平(P<0.05,下同);g.1341674C>G位点CC基因型个体则表现为第一胎、第三胎及三胎联合产羔数均高于GG基因型个体,同样在第一胎达显著水平。g.1341674C>G位点GG基因型个体的第一胎、第三胎及三胎联合羔羊初生重均高于CC基因型个体,且在第三胎达显著水平;g.1341600A>G位点不同基因型与努比亚山羊各胎次羔羊初生重无显著关联(P>0.05,下同)。在羔羊断奶重方面,2个SNP位点不同基因型与努比亚山羊各胎次断奶重也无显著关联。g.1341600A>G位点和g.1341674C>G位点可形成连锁不平衡单倍型模块,其中GC、AC、AG单倍型的频率分别为0.645、0.265和0.090;单倍型模块与第一胎产羔数显著关联,AACC组合型产羔数显著低于其他组合型。【结论】努比亚山羊KISS1基因内含子1存在2个SNP位点(g.1341600A>G和g.1341674C>G)与其繁殖性状相关,其中g.1341600A>G位点的A等位基因和g.1341674C>G位点的C等位基因可作为努比亚山羊选育的潜在分子标记,且在今后的育种过程中应淘汰KISS1基因AACC组合型母羊。

     

    Abstract: 【Objective】Clarifying the relationship between transfer inhibin gene(KISS1) polymorphism and reproductive traits(litter size, birth weight and weaning weight) in Nubian goats, so as to provide reference basis for further molecular marker assisted selection breeding of goats.【Method】In this study, the SNP sites of KISS1 gene in Nubian goats were screened by DNA mixing pool and Sanger sequencing. Then 355 Nubian goats were genotyped by MTA-Seq sequencing. The association between SNP sites of KISS1 gene and lambing phenotypic traits of Nubian goats was analyzed by SAS 9.2 general linear model(GLM).【Result】The results showed that two SNP of g.1341600A>G and g.1341674C> G were found in KISS1 gene intron. Population genetic analysis showed that the g. 1341600A>G locus was moderately polymorphic(0.25<P<0.50), and the test showed that the g.1341600A>G locus was in Hardy-Weinberg equilibrium(PHWE> 0.05), while the g.1341674C>G site was low polymorphism(PIC<0.25), and test showed that the g.1341600A>G locus was not in Hardy-Weinberg equilibrium(PHWE<0.05).Association analysis showed that at g.1341600A>G locus, the first, second and total parity litter size of GG and AG genotypes were higher than those of AA genotypes and reached significant level at the first parity litter size(P<0.05, the same below). At g.1341674C>G locus, the first, third and total parity litter size of CC genotypes were higher than those of GG genotypes and reached significant level at the first parity litter size.At g.1341674C>G locus, the first, third parity and total parity birth weight of GG genotypes were higher than those of CC genotypes, and reached significant level at the third parity birth weight.There was no significant difference among different genotypes of Nubian goats in diverse parities birth weight at g.1341600A>G(P>0.05, the same below). There was no significant difference among different genotypes of Nubian goats in diverse parities weaning weight at g.1341600A>G and g. 1341674C>G. The results of linkage disequilibrium and haplotype analysis showed that g. 1341600 C>G locus and g. 1341674C>G locus could form linkage disequilibrium module, and the frequencies of GC, AC and AG were 0.645, 0.265 and 0.090, respectively. The correlation analysis between haplotype combination and reproductive traitsshowed that there was a significant correlation between haplotype combination and the first parity litter size, and the litter size of AACC was significantly lower than those of other genotypes.【Conclusion】There are two SNP loci in KISS1 gene intron (g. 1341600A>G and g. 1341674C>G), which are related to their reproductive traits in Nubian goats. The A allele of g.1341600A>G and the C allele of g.1341674C>G can be used as potential molecular markers for Nubian goat breeding, and the AACC combination ewes of KISS1 gene should be eliminated in the breeding process in the future.

     

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