Identification and evaluation for heat tolerance at the flowering stage in different rice genotypes
-
摘要: 【目的】筛选花期耐热性强、产量水平高的水稻品种,为水稻花期耐热性鉴定与评价及应对高温热害的品种选择提供理论依据和技术参考。【方法】在人工气候室内模拟高温胁迫(高温38℃,每天处理6 h,连续处理3 d),以Y两优1号为对照品种,以相对耐热指数为评价指标,同时结合产量水平,对100个不同水稻基因型的花期耐热性进行鉴定与评价。【结果】不同水稻基因型的花期耐热性差异明显,高温胁迫下颖花结实率为3.6%~64.3%,相对结实率为9.5%~80.0%。以相对耐热指数为评级标准,耐热性级别达到1(强)、3(较强)、5(中等)、7(较弱)和9(弱)级的水稻品种数量分别为4、13、42、26和15份。采用聚类分析法将100个基因型分为6种不同耐热性和产量水平的水稻类型,其中第一类在高温热害易发区推广种植安全生产性高。相对结实率是不同水稻基因型分类的主要因子,其次为产量,根据相对结实率和产量之间的二维象限分布特征,进一步筛选出耐热性较强、产量水平较高等综合性状好的品种。【结论】通过对100个不同水稻基因型花期耐热性进行鉴定和评价,筛选出特优837、云光14号、Q优8号、国稻7号、Y两优865、汕优63和黄华占等7个耐热性较强且产量水平较高的品种,这些品种可在高温易发区推广应用。Abstract: 【Objective】In order to provide theoretical basis and technical reference for the identification and evaluation of the heat tolerance during the flowering stage and the selection of varieties to cope with high temperature and heat injury, rice varieties with high heat tolerance and high yield at flowering stage were selected in this study.【Method】In this study, the heat tolerance of 100 different rice genotypes at the flowering stage was evaluated by simulating high temperature(38℃ for 6 hours per day for 3 d) in a phytotron, using Y Liangyou 1 as the control variety and the relative heat tolerance index as the evaluation index, and also combining with the yield level.【Result】The results showed that there were great differences in spikelet fertility among different rice cultivars under heat stress at the flowering stage, demonstrating that they had different heat tolerance. Under high temperature stress, the spikelet fertility ranged from 3.6% to 64.3%, while the relative spikelet fertility ranged from 9.5% to 80.0%. The heat tolerance of different cultivars was classified according to the relative heat tolerance index, and the number of rice varieties with heat tolerance grade of 1(highly tolerant), 3(tolerant), 5(moderately tolerant), 7(susceptible) and 9(highly susceptible) were 4, 13, 42, 26 and 15, respectively. Cluster analysis was used to divide 100 rice genotypes into six types with different heat tolerance and yield levels. Among them, the first type was popularized in heat injury prone areas with high safety and productivity. Relative spikelet fertility was the main factor for the classification of different rice genotypes, followed by the yield. According to the two-dimensional quadrant distribution characteristics between relative spikelet fertility and yield, some varieties with good comprehensive traits, including strong heat tolerance and high yield level, were further screened out.【Conclusion】Through the identification and evaluation of heat tolerance of 100 different rice genotypes during the flowering time, seven varieties with high heat tolerance and high yield level are selected, such as Teyou 837, Yunguang 14, Q You 8, Guodao 7, Y Liangyou 865, Shanyou 63 and Huanghuazhan. These varieties can be planted in the high temperature prone areas.
-
Keywords:
- rice /
- flowering stage /
- heat tolerance /
- relative heat tolerance index /
- identification
-
-
曹云英, 段骅, 杨立年, 王志琴, 刘立军, 杨建昌. 2009. 抽穗和灌浆早期高温对耐热性不同籼稻品种产量的影响及其生理原因[J]. 作物学报, 35(3):512-521.[Cao Y Y, Duan H, Yang L N, Wang Z Q, Liu L J, Yang J C. 2009. Effect of high temperature during heading and early grain filling on grain yield of Indica rice cultivars differing in heat-tolerance and its physiological mechanism[J]. Acta Agronomica Sinica, 35(3):512-521.]doi: 10.3724/SP.J.1006.2009.00512. 陈刚, 吴文革, 许有尊, 周永进. 2014. 杂交中籼水稻花穗期耐热性品种筛选及鉴定指标评价[J]. 作物杂志, (5):80-85.[Chen G, Wu W G, Xu Y Z, Zhou Y J. 2014. Screening varieties of heat tolerance at flowering stage and evaluating identification indexes of hybrid indica rice[J]. Crops, (5):80-85.]doi: 10.16035/j.issn.1001-7283.2014.05.044. 段骅, 傅亮, 剧成欣, 刘立军, 杨建昌. 2013. 氮素穗肥对高温胁迫下水稻结实和稻米品质的影响[J]. 中国水稻科学, 27(6):591-602.[Duan H, Fu L, Ju C X, Liu L J, Yang J C. 2013. Effects of application of nitrogen as panicle-promoting fertilizer on seed setting and grain quality of rice under high temperature stress[J]. China Journal of Rice Science, 27(6):591-602.]doi: 10.3969/j.issn.1001-7216.2013.06.005. 郭建茂, 吴越, 杨沈斌, 江晓东, 谢晓燕, 王锦杰, 申双和. 2017. 典型高温年不同播期一季稻产量差异及其原因分析[J]. 中国农业气象, 38(2):121-130.[Guo J M, Wu Y, Yang S B, Jiang X D, Xie X Y, Wang J J, Shen S H. 2017. Yield differences and its causes for one season rice under different sowing dates in typical high temperature year[J]. Chinese Journal of Agrometeorology, 38(2):121-130.]doi: 10.3969/j.issn.1000-6362.2017.02.007. 胡声博, 张玉屏, 朱德峰, 林贤青, 向镜. 2012. 杂交水稻耐热性评价[J]. 中国水稻科学, 26(6):751-756.[Hu S B, Zhang Y P, Zhu D F, Lin X Q, Xiang J. 2012. Evaluation of heat resistance in hybrid rice[J]. China Journal of Rice Science, 26(6):751-756.]doi: 10.3969/j.issn.1001-7216.2012.06.017. 梁天锋, 王强, 陈雷, 张晓丽, 吕荣华, 陶伟, 唐茂艳. 2016. 基于时积温的水稻耐热性鉴定方法研究[J]. 南方农业学报, 47(4):537-541.[Liang T F, Wang Q, Chen L, Zhang X L, Lü R H, Tao W, Tang M Y. 2016. Identification method for heat resistance of rice based on accumulated hourly temperature[J]. Journal of Southern Agriculture, 47(4):537-541.]doi: 10.3969/j:issn.2095-1191.2016.04.537. 林翠香, 倪大虎, 宋丰顺, 胡宗兵, 甘泉. 2020. 高温胁迫下水稻生理特性变化及适应机制研究进展[J]. 安徽农学通报, 26(24):37-42.[Lin C X, Ni D H, Song F S, Hu Z B, Gan Q. 2020. Research progress on physiological cha-racteristics and adaptation mechanism of rice under high temperature stress[J]. Anhui Agricultural Science Bulle-tin, 26(24):37-42.]doi: 10.3969/j.issn.1007-7731.2020.24.012. 刘业涛, 穆麒麟, 王毅, 高园, 田小海. 2019. 从非洲水稻材料中筛选耐高温种质资源[J]. 中国农学通报, 35(12):8-12.[Liu Y T, Mu Q L, Wang Y, Gao Y, Tian X H. 2019. Selecting high temperature tolerant germplasms from Africa rice[J]. Chinese Agricultural Science Bulletin, 35(12):8-12.] 史培华. 2014. 花后高温对水稻生长发育及产量形成影响的研究[D]. 南京:南京农业大学.[Shi P H. 2014. Effects of post-anthesis heat stress on rice growth, development and yield formation[D]. Nanjing:Nanjing Agricultural University.] 陶龙兴, 谈惠娟, 王熹, 曹立勇, 宋建, 程式华. 2008. 高温胁迫对国稻6号开花结实习性的影响[J]. 作物学报, 34(4):669-674.[Tao L X, Tan H J, Wang X, Cao L Y, Song J, Cheng S H. 2008. Effects of high-temperature stress on flowering and grain-setting characteristics of Guodao 6[J]. Acta Agronomica Sinica, 34(4):669-674.]doi: 10.3321/j.issn:0496-3490.2008.04.01. 王强, 陈雷, 张晓丽, 梁天锋, 高国庆, 莫彬, 吕荣华, 陶伟, 唐茂艳. 2019. 华南优质稻花期耐热性鉴定研究[J]. 中国稻米, 25(3):80-82.[Wang Q, Chen L, Zhang X L, Liang T F, Gao G Q, Mo B, Lü R H, Tao W, Tang M Y. 2019. Evaluation of heat tolerance at the flowering stage for high quality rice in southern China[J]. China Rice, 25(3):80-82.]doi: 10.3969/j.issn.1006-8082.2019.03.017. 文绍山, 张林, 焦峻. 2018. 抽穗开花期耐热性水稻恢复系种质的筛选[J]. 贵州农业科学, 46(3):7-10.[Wen S S, Zhang L, Jiao J. 2018. Screening rice restorer line germplasm with heat tolerance at heading-flowering stage[J]. Guizhou Agricultural Sciences, 46(3):7 -10.] doi: 10.3969/j.issn.1001-3601.2018.03.00. 杨梯丰, 张少红, 王晓飞, 黄章慧, 赵均良, 张桂权, 刘斌. 2012. 水稻抽穗开花期耐热种质资源的筛选鉴定[J]. 华南农业大学学报, 33(4):585-588.[Yang T F, Zhang S H, Wang X F, Huang Z H, Zhao J L, Zhang G Q, Liu B. 2012. Screening for germplasm with heat tolerance at flowering stage in Oryza sativa[J]. Journal of South China Agricultural University, 33(4):585-588.] 杨永杰, 符冠富, 熊杰, 乐明凯, 陶龙兴. 2012. 高温对水稻的影响及水稻耐热性测评方法研究[J]. 中国稻米, 18(1):39-40.[Yang Y J, Fu G F, Xiong J, Le M K, Tao L X. 2012. A preliminary study on heat injury evaluation process for rice[J]. China Rice, 18(1):39-40.]doi: 10.3969/j.issn.1006-8082.2012.01.008. 张斌, 杨昕霞, 袁志辉. 2021. 水稻响应热胁迫核心基因的筛选与鉴定[J]. 江苏农业学报, 37(4):817-822.[Zhang B, Yang X X, Yuan Z H. 2021. Screening and identification of core genes responding to heat stress in rice[J]. Jiangsu Journal of Agricultural Sciences, 37(4):817-822.]doi: 10.3969/j.issn.1000-4440.2021.04.001. 张德文, 汪婉琳, 张伟. 2020. 江淮地区水稻耐热性鉴定技术研究[J]. 安徽农业科学, 48(12):20-22.[Zhang D W, Wang W L, Zhang W. 2020. Study on identification technology of rice heat tolerance in Jianghuai region[J]. Journal of Anhui Agricultural Sciences, 48(12):20-22.]doi: 10.3969/j.issn.0517-6611.2020.12.006. 张桂莲, 张顺堂, 肖浪涛, 武小金, 肖应辉, 陈立云. 2013. 高温胁迫对水稻剑叶保护酶活性和膜透性的影响[J]. 作物学报, 39(1):177-183.[Zhang G L, Zhang S T, Xiao L T, Wu X J, Xiao Y H, Chen L Y. 2013. Effect of high temperature stress on physiological characteristics of anther and pollen traits of rice at flowering stage[J]. Acta Agronomica Sinica, 39(1):177-183.]doi: 10.3321/j.issn:0496-3490.2006.09.007. 赵森, 于江辉, 周浩, 孟秋成, 肖国樱. 2013. 抽穗开花期耐高温的爪哇稻资源筛选[J]. 植物遗传资源学报, 14(3):384-389.[Zhao S, Yu J H, Zhou H, Meng Q C, Xiao G Y. 2013. Screening of Javanica rice for thermo-tolerance at heading stage[J]. Journal of Plant Genetic Resources, 14(3):384-389.]doi: 10.13430/j.cnki.jpgr.2013.03.006. 周建霞, 胡声博, 张玉屏, 朱德峰, 林贤青, 陈惠哲, 向镜. 2013. 早稻花期高温鉴定初探[J]. 中国稻米, 19(4):28-29.[Zhou J X, Hu S B, Zhang Y P, Zhu D F, Lin X Q, Chen H Z, Xiang J. 2013. Preliminary study on identification of high temperature at flowering stage in early rice[J]. China Rice, 19(4):28-29.]doi: 10.3969/j.issn.1006-8082.2013.04.008. Aghamolki M T K, Yusop M K, Oad F C, Zakikhani H, Jaafar H, Kharidah S, Hanafi M M. 2014. Heat stress effects on yield parameters of selected rice cultivars at reproductive growth stage[J]. Journal of Food, Agriculture and Environment, 12(2):741-746.
Bahuguna R N, Jha J, Pal M, Shah D, Lawas L M F, Khetarpal S, Jagadish S V K. 2015. Physiological and biochemical characterization of NERICA-L-44:A novel source of heat tolerance at the vegetative and reproductive stages in rice[J]. Physiologia Plantarum, 154(4):543-559. doi: 10.1111/ppl.12299.
Chen L, Wang Q, Tang M Y, Zhang X L, Pan Y H, Yang X H, Gao G Q, Lv R H, Tao W, Jiang L G, Liang T F. 2021. QTL mapping and identification of candidate genes for heat tolerance at the flowering stage in rice[J]. Frontiers in Genetics, 11:621871. doi: 10.3389/fgene2020.621871.
Coast O, Ellis R H, Murdoch A J, Quiñ ones C I, Jagadish S V K. 2015. High night temperature induces contrasting responses for spikelet fertility, spikelet tissue temperature, flowering characteristics and grain quality in rice[J]. Functional Plant Biology, 42:149-161. doi: 10.1071/FP14104.
Dar M H, Bano D A, Waza S A, Zaidi N W, Majid A, Shikari A B, Ahangar M A, Hossain M, Kumar A, Singh U S. 2021. Abiotic stress tolerance-progress and pathways of sustainable rice production[J]. Sustainability, 13:2078. doi: 10.3390/su13042078.
Hakata M, Wada H, Masumoto-Kubo C, Tanaka R, Sato H, Morita S. 2017. Development of a new heat tolerance assay system for rice spikelet sterility[J]. Plant Methods, 13:34. doi: 10.1186/s13007-017-0185-3.
IPCC. 2014. Climate change(2014):Synthesis report. In core writing team, Pachauri R K, Meyer L A(Eds.), Contribution of working groups Ⅰ, Ⅱ and Ⅲ to the fifth assessment report of the intergovernmental panel on climate change[M]. Geneva, Switzerland:Cambridge University Press.
IRRI. 2013. Standard evaluation system for rice(SES)[R]. Manila, the Philippines:International Rice Research Institute, 5:35-36.
Jagadish S V K, Cairns J, Lafitte R, Wheeler T R, Price A H, Craufurd P Q. 2010. Genetic analysis of heat tolerance at anthesis in rice[J]. Crop Science, 50:1633-1641. doi: 10.2135/cropsci2009.09.0516.
Jagadish S V K, Craufurd P Q, Wheeler T R. 2007. High tem-perature stress and spikelet fertility in rice(Oryza sativa L.)[J]. Journal of Experimental Botany, 58(7):1627-1635. doi: 10.1093/jxb/erm003.
Jagadish S V K, Craufurd P Q, Wheeler T R. 2008. Phenotyping parents of mapping populations of rice for heat toleran-ce during anthesis[J]. Crop Science, 48(3):1140-1146. doi: 10.2135/cropsci2007.10.0559.
Jagadish S V K. 2020. Heat stress during flowering in cereals-effects and adaptation strategies[J]. New Phytologist, 226(6):1567-1572. doi: 10.1111/nph.16429.
Mangrauthia S K, Agarwal S, Sailaja B, Sarla N, Voleti S R. 2016. Transcriptome analysis of Oryza sativa(rice) seed germination at high temperature shows dynamics of genome expression associated with hormones signalling and abiotic stress pathways[J]. Tropical Plant Biology, 9(4):215-228. doi: 10.1007/s12042-016-9170-7.
Maruyama A, Weerakoon W M W, Wakiyama Y, Ohba K. 2013. Effects of increasing temperatures on spikelet fertility in different rice cultivars based on temperature gradient chamber experiments[J]. Journal of Agronomy and Crop Science, 199(6):416-423. doi: 10.1111/jac.12028.
Nguyen D N, Lee K J, Kim D I, Anh N T, Lee B W. 2014. Modeling and validation of high-temperature induced spikelet sterility in rice[J]. Field Crops Research, 156:293-302. doi: 10.1016/j.fcr.2013.11.009.
Prasad P V V, Boote K J, Allen Jr L H, Sheehy J E, Thomas J M G. 2006. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress[J]. Field Crops Research, 95(2-3):398-411. doi: 10.1016/j.fcr.2005.04.008.
Scafaro A P, Haynes P A, Atwell B J. 2010. Physiological and molecular changes in Oryza meridionalis Ng., a heattolerant species of wild rice[J]. Journal of Experimental Botany, 61(1):191-202. doi: 10.1093/jxb/erp294.
Shi W J, Ishimaru T, Gannaban R B, Oane W, Jagadish K S V. 2014. Popular rice cultivars show contrasting responses to heat stress at gametogenesis and anthesis[J]. Crop Science, 55:589-596. doi: 10.2135/cropsci2014.01.0054.
Tao F L, Zhang S, Zhang Z. 2013. Changes in rice disasters across China in recent decades and the meteorological and agronomic causes[J]. Regional Environmental Change, 13:743-759. doi: 10.1007/s10113-012-0357-7.
Tenorio F A, Ye C R, Redoñ a E, Sierra S, Laza M, Argayoso M A. 2013. Screening rice genetic resources for heat tolerance[J]. SABRAO Journal of Breeding and Genetics, 45(3):371-381.
Wahid A S, Gelani S, Ashraf M, Foolad M. 2007. Heat tolerance in plants:An overview[J]. Environmental and Experimental Botany, 61(3):199-223. doi: 10.1016/J.ENV-EXPBOT.2007.05.011.
Xiao Y, Pan Y, Luo L, Zhang G, Deng H, Dai L, Liu X, Tang W, Chen L, Wang G L. 2011. Quantitative trait loci associated with seed set under high temperature stress at the flowering stage in rice(Oryza sativa L.)[J]. Euphytica, 178:331-338. doi: 10.1007/s10681-010-0300-2.
Yang Z Y, Zhang Z L, Zhang T, Fahad S, Cui K H, Nie L X, Peng S B, Huang J L. 2017. The effect of season-long temperature increases on rice cultivars grown in the central and southern regions of China[J]. Frontiers in Plant Science, 8:1908. doi: 10.3389/fpls.2017.01908.
-
期刊类型引用(6)
1. 从夕汉,阮新民,施伏芝,杜弘杨,罗彦长,罗玉祥,罗志祥. 504S系列组合产量·品质及抗逆性的综合表现. 安徽农业科学. 2024(16): 40-42 . 百度学术
2. 黄伦霄,吴佳宏,秦鱼河,韩佳佳,蒋菲,魏红宇,陈云澈,彭春霖,向警,赵正武. 穗期高温处理赣早籼58与周南稻杂交F_(2:3)家系对农艺性状的影响. 江苏农业科学. 2023(05): 103-109 . 百度学术
3. 徐晓征,施俊生,游兆彤,王建军,王仁杯,崔永涛,宋建,唐立群. 应对极端气候的水稻品种选择与育种技术对策. 浙江农业科学. 2023(04): 786-790 . 百度学术
4. 张杨,龚林忠,艾小艳,朱炜,王会良,刘勇,诸小敏,王富荣,何华平. 137份桃种质资源耐热性评价. 南方农业学报. 2023(02): 467-475 . 本站查看
5. 苏小雨,高桐梅,张鹏钰,李丰,吴寅,王东勇,田媛,卫双玲. 基于主成分分析及隶属函数法对芝麻苗期耐热性综合评价. 作物杂志. 2023(04): 52-59 . 百度学术
6. 伍琼,陈曦,曹强,陈砚涛,岳伟. 1961—2020年大别山区农业气候资源变化特征分析. 河南农业科学. 2022(02): 75-85 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 83
- HTML全文浏览量: 4
- PDF下载量: 4
- 被引次数: 7