广西砂梨地方品种果实品质性状分析及综合评价

Fruit quality character analysis and comprehensive evaluation of Pyrus prifolia Nakai landrace in Guangxi

  • 摘要: 【目的】对国家果树种质武昌砂梨圃保存的49份广西砂梨地方品种的果实品质性状进行分析和综合评价,为挖掘利用广西砂梨地方品种优异资源提供依据。【方法】分析12个果实品质性状的变异系数、Shannon-Weaver指数等指标,应用聚类分析对品种和性状进行分类,并应用主成分分析进行果实品质的综合评价。【结果】广西砂梨地方品种的果实品质性状的变异系数范围为9.25%~52.57%,其Shannon-Weaver指数(H')范围在1.827~2.037。单果重、果实纵径、果实横径和果形指数的均值分别为223.2 g、7.19 cm、7.18 cm和1.00,变异系数分别为33.20%、16.9%、11.26%和12.24%,H'分别是1.851、1.965、1.907和1.949。果肉硬度、可溶性固形物、可溶性糖、可滴定酸、维生素C和石细胞含量的均值分别为12.00 kg/cm2、10.49%、5.63%、0.31%、1.75 mg/100 g和0.90%,变异系数分别为14.40%、9.25%、15.18%、52.57%、18.25%和35.18%,H'分别为1.957、2.037、1.935、1.910、1.827和1.933,其中可溶性固形物含量的H'(2.037)最大,可滴定酸含量变异系数(52.57%)最大,多样性最丰富。固酸比和糖酸比的均值分别为42.73和22.65,变异系数分别为45.50%和44.76%,H'分别为1.956和1.848。R型聚类将12个梨果实品质性状聚成4类。经相关分析及R型聚类分析表明,果实品质部分性状之间的逻辑相关性明显,多数性状之间存在着显著(P<0.05)或极显著(P<0.01)的相关性。通过主成分分析提取了4个主成分,可反映原始数据80.424%的信息量;建立基于主成分的梨果实品质综合评价模型,筛选获得9份综合得分在1.000以上的地方品种。采用Q聚类将广西49个砂梨地方品种分为3大类,分别对应品质优良、品质中等和品质差3个类型。【结论】广西砂梨地方品种果实品质性状具有丰富的多样性,发掘出1份大果型特异种质苍悟大砂梨,可作为大果型特异资源进行开发利用;通过建立基于主成分的梨果实品质综合评价模型评价广西砂梨地方品种的果实品质结果可靠,可为砂梨地方品种果实品质综合评价提供一定的理论参考。

     

    Abstract: 【Objective】 To promote the utilization of sand pear(Pyrus prifolia Nakai)germplasm of Guangxi landraces,twelve characters were analyzed and evaluated from 49 sand pear landraces maintained at the National Sand Pear Germplasm Repository.【Method】 The coefficient of variations and Shannon-Weaver indexes of 12 fruit quality characters were analyzed. Q cluster and R cluster analyses were carried out to classify germplasm resources and traits and principal component analysis was used to evaluate fruit quality.【Result】 The coefficients of variation of fruit quality traits in Guangxi landraces varied between 9.25% to 52.57% and their Shannon-Weaver indexes ranged from 1.827 to 2.037. The average values of single fruit weight,fruit diameter,fruit length and fruit shape index were 223.2 g,7.19 cm,7.18 cm and 1.00,respectively. The corresponding coefficient of variations for these were 33.20%,16.9%,11.26% and 12.24% and the Shannon Weaver diversity indexes were 1.851,1.965,1.907 and 1.949,respectively. The average values of flesh hardness,soluble solids,soluble sugar,titratable acid,Vitamin C and stone cell content were 12.00 kg/cm2,10.49%,5.63%,0.31%,1.75 mg/100 g and 0.90%,respectively. The coefficient of variations for such were 14.40%,9.25%,15.18%,52.57%,18.25% and 35.18% and the Shannon Weaver diversity indexes were 1.957,2.037,1.935,1.910,1.827 and 1.933,respectively. The soluble solids had the largest Shannon Weaver index(2.037),whereas the titratable acid content had the largest coefficient of variation(52.57%)and the richest diversity. The average values of solid acid ratio and sugar acid ratio were 42.73 and 22.65 with coefficients of variation of 45.50% and 44.76% and Shannon Weaver diversity indexes of 1.956 and 1.848. The 12 fruit quality traits were divided into 4 groups by R cluster analysis. The correlation analysis and R-type cluster analysis revealed that fruit quality displayed logical correlation between some traits and significant(P<0.05)or highly significant(P<0.01)correlations were observed among most traits. Four principal components were extracted from our principal component analysis and it covered 80.424% of the original data. A comprehensive evaluation model was established by principal component analysis and 9 cultivars were selected with a score above 1.000.The 49 sand pear landraces were divided into three groups by Q-clustering,corresponding to good,medium and poor quality.【Conclusion】 Abundant genetic diversity was observed in Guangxi landraces of sand pear through analyses of fruit quality traits. The results from the evaluation of sand pear fruit quality by principal component analysis in Guangxi landraces proved to be reliable and the evaluation model developed provides a theoretical reference for future comprehensive evaluations of fruit quality in sand pear landraces.

     

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