螯合剂对青葙吸收富集土壤中镉、铅、锌和铜的影响

Effects of chelators on the uptake and accumulation of cadmium,lead,zinc and copper in soil by Celosia argentea L.

    摘要
  • 摘要: 【目的】探究螯合剂乙二胺二琥珀酸（EDDS）、氨三乙酸（NTA）和乙二醇二乙醚二胺四乙酸（EGTA）对青葙镉（Cd）、铅（Pb）、锌（Zn）和铜（Cu）吸收累积的影响，为螯合剂和青葙在环境修复中的应用提供数据支撑。【方法】采用大棚内盆栽种植青葙试验方法，以不添加螯合剂作对照（CK），研究螯合剂EDDS（1.0、2.0和3.0 mmol/kg）、NTA（1.0、2.0和3.0 mmol/kg）和EGTA（1.0、2.0和3.0 mmol/kg）作用下青葙对土壤Cd、Pb、Zn和Cu的吸收与转运。【结果】与CK相比，1.0和2.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA、1.0和2.0 mmol/kg EGTA能显著促进青葙生长（P<0.05，下同），其中1.0 mmol/kg EGTA促进效果最大，其干重较CK增加37.5%。1.0和2.0 mmol/kg EDDS、2.0和3.0 mmol/kg NTA、1.0和2.0 mmol/kg EGTA能显著促进青葙对Cd的吸收累积，且2.0 mmol/kg EGTA作用下，青葙中Cd的生物富集系数和转运系数提升最大，分别是CK的1.55和1.61倍；2.0和3.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA及1.0~3.0mmol/kg EGTA均能显著促进青葙对Pb的吸收累积，且Pb在青葙中的生物富集系数和转运系数较CK均有明显提升，以3.0 mmol/kg EGTA处理的青葙地上部富集系数和转移系数最大，分别是CK的1.63和1.47倍；1.0和2.0 mmol/kg EDDS、2.0 mmol/kg EGTA能显著促进青葙对Zn的吸收累积，以1.0 mmol/kg EDDS处理的富集系数最大，是CK的1.18倍，2.0 mmol/kg NTA处理的转移系数最大，是CK的1.22倍；1.0~3.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA和1.0~3.0 mmol/kg EGTA均能显著促进青葙对Cu的吸收累积，以2.0 mmol/kg EDDS处理的青葙地上部富集系数最大，是CK的2.07倍，3.0 mmol/kg EGTA处理的青葙地上部转移系数最大，是CK的2.52倍。【结论】在本研究螯合剂施用剂量范围内，除3.0 mmol/kg EGTA减少青葙吸收累积Cd和3.0 mmol/kg EDDS、3.0 mmol/kg NTA、1.0 mmol/kg EGTA及3.0 mmol/kg EGTA减少青葙吸收累积Zn外，3种螯合剂的其余剂量均可促进青葙对Cd、Pb、Zn和Cu的吸收富集。因此，适当剂量的螯合剂作为诱导活化剂，可强化青葙吸收累积重金属Cd、Pb、Zn和Cu。

     

    Abstract: 【Objective】 The experimental objective was to investigate the effects of chelatorsethylene diamine disuccinate(EDDS),nitrilotriacetic acid(NTA)and glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid(EGTA)on the uptake and accumulation of cadmium(Cd),lead(Pb),zinc(Zn)and copper(Cu)with Celosia argentea L.,and to provide data support for the application of chelators and C. argentea L. in environmental repairation.【Method】 The application effects of EDDS(1.0,2.0 and 3.0 mmol/kg),NTA(1.0,2.0 and 3.0 mmol/kg)and EGTA(1.0,2.0 and 3.0 mmol/kg)were evaluated on the uptake and transportation of heavy metals(Cd,Pb,Zn and Cu)from soil with C. argentea L. by pot experiment in greenhouse,and without the addition of chelating agent as contrast(CK).【Result】 The results indicated that 1.0-2.0 mmol/kg EDDS,1.0-3.0 mmol/kg NTA,1.0-2.0 mmol/kg EGTA promoted significantly the growth of C. argentea L.(P<0.05,the same below)compared with CK,and among them,1.0 mmol/kg EGTA had the best effect,the dry weight increased by 37.5% than that of CK. 1.0-2.0 mmol/kg EDDS,2.0-3.0 mmol/kg NTA,1.0-2.0 mmol/kg EGTA significantly promoted the absorption and accumulation of Cd in C. argentea L.,and the highest bioconcentration factors and translocation factor were respectively 1.55 and 1.61 times as those of CK with applying 2.0 mmol/kg EGTA. 2.0-3.0 mmol/kg EDDS,1.0-3.0 mmol/kg NTA and 1.0-3.0 mmol/kg EGTA could significantly promote the absorption and accumulation of Pb in C. argentea L.,and the bioconcentration factors and translocation factor of Pb in C. argentea L. were improved compared with CK,and the highest bioconcentration factors and translocation factor were respectively 1.63 and 1.47 times as those of CK with applying 3.0 mmol/kg EGTA. 1.0 and 2.0 mmol/kg EDDS and 2.0 mmol/kg EGTA could significantly promote the absorption and accumulation of Zn in C. argentea L.,and the highest bioconcentration factor was as 1.18 times as that of CK when applying 1.0 mmol/kg EDDS. With applying 2.0 mmol/kg NTA,and the highest translocation factor was as 1.22 times as that of CK. With applying 1.0-3.0 mmol/kg EDDS,1.0-3.0 mmol/kg NTA and 1.0-3.0 mmol/kg EGTA could significantly promote the absorption and accumulation of Cu in C. argentea L.,and the highest bioconcentration factor was as 2.07 times as that of CK with applying 2.0 mmol/kg EDDS,and the highest translocation factor was as 2.52 times as that of CK with applying 3.0 mmol/kg EGTA.【Conclusion】 In this study,three chelators EDDS,NTAE and EGTA in a suitable dose can promote the absorption and accumulation of Cd,Pb,Zn and Cu in C. argentea L.(except 3.0 mmol/kg EGTA reduces the absorption and accumulation of Cd,and except 3.0 mmol/kg EDDS,3.0 mmol/kg NTA,1.0 mmol/kg EGTA and 3.0 mmol/kg EGTA reduces the absorption and accumulation of Zn). Therefore,the suitable dose of the three chelators as inductive activators can enhance absorption and accumulation of heavy metals Cd,Pb,Zn and Cu in C. argentea L.

     

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