氰戊菊酯等4种化学药物对克氏原螯虾的急性毒性及组织病理观察

Acute toxicity of four chemical drugs including fenvalerate to Procambarus clarkii and the histopathological observation

  • 摘要: 【目的】评价氰戊菊酯、辛硫磷、硫醚沙星和甲霜灵对克氏原螯虾(Procambarus clarkii)的急性毒性,分析其对克氏原螯虾的组织病理影响,为生态风险评估提供基础数据。【方法】采用静态试验法测定4种药物浸泡下克氏原螯虾的急性毒性作用,分别使用4种药物48 h半致死浓度(LC50)和96 h-LC50的10%浓度浸泡克氏原螯虾48和96 h后取样,基于石蜡切片技术比较分析4种药物对克氏原螯虾肝胰腺和肌肉组织结构的影响。【结果】4种药物浸泡下表现的中毒症状比较类似,且不同浓度组的中毒症状表现出明显的剂量和时间效应。急性毒性试验结果表明,4种化学药物对克氏原螯虾的毒性排序为氰戊菊酯>辛硫磷>硫醚沙星>甲霜灵。氰戊菊酯对克氏原螯虾24、48和96 h的LC50分别为4.375、4.252和3.865μg/L,辛硫磷对克氏原螯虾24、48和96 h的LC50分别为161.301、87.784和81.637μg/L,硫醚沙星对克氏原螯虾24、48和96 h的LC50分别为76.541、72.509和52.529 mg/L,甲霜灵对克氏原螯虾24、48和96 h的LC50分别为20.610、5.499和4.141 g/L。组织病理观察发现,以相对低浓度的化学药物染毒克氏原螯虾后,仍会对克氏原螯虾的组织产生损伤和破坏。染毒后的肝胰腺发现部分细胞破裂、皱缩、坏死,B细胞及其内部转运泡体积明显增大且增多,R细胞颜色加深等现象;肌肉整体呈现肌纤维断裂溶解、细胞排列不紧密、出现明显空腔等现象。【结论】氰戊菊酯、辛硫磷、硫醚沙星和甲霜灵对克氏原螯虾的急性毒性具有较大差异。克氏原螯虾在4种化学药物的安全浓度下均能够存活,但会对肝胰腺和肌肉组织产生破坏。

     

    Abstract: 【Objective】In order to evaluate the acute toxicity of fenvalerate, phoxim, sulfide gatifloxacin and ridomil to Procambarus clarkii, analyze the histopathological effects on P. clarkii, and provide basic data for ecological risk assessment.【Method】The acute toxicity effects of four drugs on P. clarkii were determined by static test method. P. clarkii were soaked in four drugs for 48 and 96 h with 10% concentration of 48 h half lethal concentration(LC50) and 10% concentration of 96 h-LC50 respectively. The changes of hepatopancreas and muscle structure of P. clarkii were compared and analyzed based on paraffin section technology.【Result】The poisoning symptoms of the four drugs were similar, and the poisoning symptoms of different concentration groups showed obvious dose and time effects. The results of acute toxicity test showed that, toxicity ranking of four chemical drugs was fenvalerate>phoxim>sulfide gatifloxacin>ridomil. The LC50 of fenvalerate to P. clarkii was 4.375, 4.252 and 3.865 μg/L at 24, 48 and 96 h. The LC50 of phoxim to P. clarkii was 161.301, 87.784 and 81.637 μg/L. 24, 48 and 96 h-LC50 of sulfide gatifloxacin to P. clarkii was 76.541, 72.509 and 52.529 mg/L. And the LC50 of ridomil for P. clarkii at 24, 48 and 96 h respectively were 20.610, 5.499 and 4.141 g/L. Histopathological observation showed that the tissues of P. clarkii were still damaged and destroyed after being exposed to relatively low concentrations of chemical drugs. The results showed that part of hepatopancreas cells ruptured, shrunk and necrosis, the volume of B cells and its internal transport vesicles increased greatly, and the color of R cells deepened;the whole muscle showed the phenomenon of breaking and dissolving of muscle fibers, loose arrangement of cells and obvious cavity.【Conclusion】The acute toxicity of fenvalerate, phoxim, sulfide gatifloxacin and ridomil to P. clarkii is different.P. clarkii can survive at the safe concentration of four chemicals, but it damages the hepatopancreas and muscle tissue.

     

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