Abstract: 【Objective】To determine the susceptibility of 89 strains of Botryosphaeriaceous isolate from major mango producing areas in China to three fungicides:prochloraz, difenoconazole and pyraclostrobin, and to provide reference for mango disease control caused by Botryosphaeriaceous isolate.【Method】Mycelial growth rate method was used to determine the sensitivity of Botryosphaeriaceous isolate collected from main mango producing areas of Guangxi, Hainan, Yunnan, Sichuan, Guangdong and Fujian to prochloraz, difenoconazole and pyraclostrobin, and DPS9.01 and SPSS20 softwares were used to analyze the sensitivity and cross resistance of the strains to the three fungicides.【Result】Among the 89 strains tested in this study, there were differences in susceptibility to prochloraz, difenoconazole and pyraclostrobin. The EC₅₀ for prochloraz ranged from 0.0048 to 38.5037 mg/L, with an average value of 2.8637 mg/L, and the maximum EC₅₀ was 8021.6 times of the minimum EC₅₀. The EC₅₀ for difenoconazole ranged from 0.0147 to 8.8935 mg/L, with an average value of 1.1761 mg/L, and the maximum EC₅₀ was 605.0 times of the minimum EC₅₀. The EC₅₀ values for pyraclostrobin ranged from 0.0195 to 145.0578 mg/L, with an average value of 8.1939 mg/L, and the maximum EC₅₀ was 7438.9 times of the minimum EC₅₀. There were differences in the susceptibility of the isolates collected from different mango producing areas to prochloraz, difenoconazole and pyraclostrobin, among which the isolates from Guangdong had the highest average EC₅₀ to prochloraz(8.1127 mg/L) and pyraclostrobin(15.7240 mg/L), and the isolates from Sichuan had the highest average EC₅₀ to difenoconazole(1.6730 mg/L). There were differences in the susceptibility of Botryosphaeriaceous isolates from different genera to prochloraz, difenoconazole and pyraclostrobin. The average EC₅₀ of the isolates in Lasiodiplodia to three fungicides was higher than that of Botryosphaeria and Neofusicoccum.【Conclusion】All the 89 strains of Botryosphaeriaceous isolates collected from different mango producing areas are more sensitive to difenoconazole, followed by prochloraz, and have the lowest sensitivity to pyraclostrobin. There is no cross-resistance to the three fungicides, and it can be reasonably mixed or applied alternately. There are differences in the sensitivity of mango Botryosphaeriaceous isolates from different sources and genera to the three fungicides, so it should be rationally and scientifically applied according to specific regions and pathogenic species in production.