Abstract: 【Objective】The study aimed to explore the effects of the probiotic combinations on the intestinal microbiota diversity and species abundance of ultra-early weaned(7 d) piglets of Duroc×Diqing Tibetan pig. Then released the stress of weaned piglets, and provided scientific basis for the development of microbial feed additives.【Method】A total of 30 piglets of Duroc×Diqing Tibetan pig, aged 7-day-old were divided into 3 groups with 10 piglets in each group. The piglets of control group(group ZM) were fed with breast milk, the piglets of experimental group I(group ZD) were fed with milk replacer and the piglets of experimental group II(group ZY) were fed with milk replacer and probiotic combinations. The experimental period was 21 d. Collected the faeces samples of the piglets in the last day of the experiment(28-day-old), then analyzed the samples fecal microbiota in structure with IIIumina high throughput sequencing technology.【Result】The IIIumina high throughput sequencing data showed that there were 327 common OTUs in the three groups, while there were 247 unique OTUs in the group ZM, 84 unique OTUs in the group ZD and 96 unique OTUs in the group ZY, respectively. At the phylum level, the relative abundance of Firmicutes in group ZM was the highest one, while that was Bacteroidetes in group ZD and group ZY. The Firmicutes/Bacteroidetes ratios were 0.77 and 0.92 of the group ZY and ZD, respectively. Compared with group ZM(1.76), they were decreased by 56.25% and 47.73%, respectively. At the genus level, the dominant genus of group ZD and ZY was Prevotella_9 in the faeces samples of the Duroc×Diqing Tibetan Pig, while that of group ZM was Lactobacillus. The relative abundance of Blautia, Enterococcus and Subdoligranulum in group ZD was significantly higher than the other two groups(P<0.05, the same below). The relative abundance of Prevotellaceae_NK3B31_group, Prevotellaceae_UCG-003, Anaerovibrio, Subdoligranulum, Ruminococcaceae_NK4A214_group, Ruminococcaceae_UCG-005, norank_f_Prevotellaceae and Family_XIII_AD3011_group in group ZY were signi-ficantly higher than the group ZD.【Conclusion】In conclusion, after feeding the milk replacer with adding the probiotic combinations, the diversity of structure of intestinal flora are increased, and the relative abundance of the microflora which is related to metabolism of carbohydrate and producing short chain fatty acids in ultra-early weaned piglets of Duroc×Diqing Tibetan pig were increased. The potential functions of the probiotic combinations which can promote nutrient metabolism and anti-inflammation can shorten the transformation process of digestive tract microflora from lactation type to feed type.