Abstract: 【Objective】Evaluating the growth characters and genetic characters of seedlings regenerated from healthy cane setts from different generations of chewing cane cultivar Guiguozhe 1 could help improve the quality of healthy cane setts and high-yielding cultivation.【Method】Phenotypic characters of seedlings from three different generations of Guiguozhe 1 including tissue culture seedling(TCS), first generation seedling(FGS) and second generation seedling(SGS) were successively measured.Growth characters and variability stability of seven traits in seedlings from three generations were analyzed, including number of seedling, plant height, ground diameter, number of leaves, and leaf length, leaf width and area of the first leaf.【Result】Growth characters were obviously different among generations.Phenotypic characters of FGS were close to SGS, and they performed better than TCS(P< 0.05), but the performance of SGS was still better than FGS.Variation coefficient average value of 7 growth characters in seedlings from three generations was 5.48%.The variance range was 3.01%-11.88%, the number of seedling had the maximum variation and the ground diameter had the minimum variation.Except for the increase in variance of the first leaf width in SGS, the variances of other six growth characters tended to stabilize along with the increasing of generations.The variance coefficient average value of all seven characters ranged from 3.32% to 8.25%, that of TCS was the largest(8.25%), FGS was in middle(4.88%) and SGS was the smallest(3.32%).The results suggest that the characters segregation of TCS was the maximum, and the variance was relatively stable in later-generation(SGS).【Conclusion】The SGS of chewing cane cultivar Guiguozhe 1 is comprehensively superior to TCS and FGS for all the growth characters, and its genetic variance is stable.SGS is recommended as the best cane setts for cultivation.